CAJ | 학술논문

目的比较不同期别煤工尘肺患者肺泡巨噬细胞自噬活动的差别.方法对116例行全肺灌洗的观察对象和煤工尘肺患者进行现场调查,并收集其肺灌洗液,纯化后得到肺巨噬细胞,用透射电镜观察其自噬形态,采用免疫印迹方法检测自噬标志性蛋白LC3及自噬调控蛋白beclin1、mTOR和p-mTOR的表达量,分析接尘观察对象、Ⅰ、Ⅱ、Ⅲ期煤工尘肺患者肺泡巨噬细胞自噬活动的强弱变化.结果透射电镜观察,发现自噬活动在不同期别煤工尘肺患者肺泡巨噬细胞中Ⅱ期煤工尘肺肺泡巨噬细胞中有自噬溶酶体明显多于观察对象和Ⅰ期煤工尘肺,Ⅱ期煤工尘肺的肺泡巨噬细胞中自噬体和自噬溶酶体明显少于Ⅱ期煤工尘肺.Ⅱ期煤工尘肺患者肺巨噬细胞LC3Ⅱ/Ⅰ和Beclin1蛋白表达量明显高于Ⅰ期,Ⅲ期煤工尘肺患者肺巨噬细胞LC3Ⅱ/Ⅰ和Beclin1蛋白表达量明显低于Ⅱ期,Ⅲ期煤工尘肺患者肺巨噬细胞LC3Ⅱ/Ⅰ蛋白表达量明显高于Ⅰ期,Ⅱ期煤工尘肺患者Beclin1蛋白表达量高于接尘观察对象,差异均有统计学意义(P＜0.05).Ⅱ期煤工尘肺患者肺泡巨噬细胞mTOR和p-mTOR的表达量明显低于接尘观察对象和Ⅰ期,Ⅲ期尘肺患者肺泡巨噬细胞mTOR和p-mTOR表达量明显高于Ⅱ期,差异均有统计学意义(P＜0.05).结论不同期别煤工尘肺患者肺泡巨噬细胞自噬活动不同.
목적 비교불동기별매공진폐환자폐포거서세포자서활동적차별.방법 대116례행전폐관세적관찰대상화매공진폐환자진행현장조사,병수집기폐관세액,순화후득도폐거서세포,용투사전경관찰기자서형태,채용면역인적방법검측자서표지성단백LC3급자서조공단백beclin1、mTOR화p-mTOR적표체량,분석접진관찰대상、Ⅰ、Ⅱ、Ⅲ기매공진폐환자폐포거서세포자서활동적강약변화.결과 투사전경관찰,발현자서활동재불동기별매공진폐환자폐포거서세포중Ⅱ기매공진폐폐포거서세포중유자서용매체명현다우관찰대상화Ⅰ기매공진폐,Ⅱ기매공진폐적폐포거서세포중자서체화자서용매체명현소우Ⅱ기매공진폐.Ⅱ기매공진폐환자폐거서세포LC3Ⅱ/Ⅰ화Beclin1단백표체량명현고우Ⅰ기,Ⅲ기매공진폐환자폐거서세포LC3Ⅱ/Ⅰ화Beclin1단백표체량명현저우Ⅱ기,Ⅲ기매공진폐환자폐거서세포LC3Ⅱ/Ⅰ단백표체량명현고우Ⅰ기,Ⅱ기매공진폐환자Beclin1단백표체량고우접진관찰대상,차이균유통계학의의(P＜0.05).Ⅱ기매공진폐환자폐포거서세포mTOR화p-mTOR적표체량명현저우접진관찰대상화Ⅰ기,Ⅲ기진폐환자폐포거서세포mTOR화p-mTOR표체량명현고우Ⅱ기,차이균유통계학의의(P＜0.05).결론 불동기별매공진폐환자폐포거서세포자서활동불동.
Objective To evaluate the differences in the autophagy activity of alveolar macrophages between patients with different stages of coal workers' pneumoconiosis (CWP).Methods A total of 116 coal workers were investigated in the field.Their lung lavage fluid was collected and purified to obtain alveolar macrophages.The morphological characteristics of autophagy were observed by transmission electron microscopy.The expression of autophagy marker (LC3) and autophagy regulators (Beclin 1,mTOR,and pmTOR) was measured by Western blot.The autophagy activity of alveolar macrophages was compared between dust-exposed subjects and patients with stage Ⅰ,Ⅱ,and Ⅲ CWP.Results The autophagy activity of alveolar macrophages differed between patients with different stages of CWP,according to transmission electron microscopy.Patients with stage Ⅱ CWP had significantly higher protein expression of LC3 Ⅱ/Ⅰ and Beclin1 in pulmonary macrophages than those with stage Ⅰ CWP (P＜0.05); patients with stage Ⅲ CWP had significantly lower protein expression of LC3 Ⅱ/Ⅰ and Beclin 1 in pulmonary macrophages than those with stage Ⅱ CWP (P＜0.05),but had significantly higher protein expression of LC3 Ⅱ/Ⅰ and Beclin 1 than those with stage Ⅰ CWP (P＜0.05);patients with stage Ⅱ CWP had a significantly higher protein expression of Beclin1 than the dust-exposed subjects (P＜0.05).Patients with stage Ⅱ CWP had significantly lower expression of mTOR and p-mTOR in pulmonary macrophages than the dust-exposed subjects and those with stage Ⅰ CWP (P＜0.05),while patients with stage Ⅲ CWP had significantly higher expression of mTOR and p-mTOR than those with stage Ⅱ CWP (P＜0.05).Conclusion The autophagy activity of alveolar macrophages varies between patients with different stages of CWP.