CAJ | 학술논문

目的探讨高压氧治疗对血红素加氧酶-1(HO-1)在糖尿病足大鼠皮肤组织中表达的影响以及HO-1水平与溃疡愈合的相关性.方法健康成年雄性Wistar大鼠45只,体质量250～350 g,按数字表法随机分为糖尿病足部溃疡组(溃疡组)、糖尿病足部溃疡高压氧治疗组(高压氧组)、正常对照组(对照组),每组15只.3组均以高脂饲料(88.8％基础饲料、1％胆固醇、10％猪油、0.2％胆盐)饲养1个月,制作成2型糖尿病模型,溃疡组和高压氧组大鼠再制作成2型糖尿病足溃疡模型,高压氧组用高压氧治疗(治疗压力0.2 MPa,1次/d,连续治疗15 d).然后取3组大鼠足部皮肤,采用逆转录聚合酶链反应(PT-PCR)检测皮肤组织中HO-1 mRNA的表达;采用免疫组织化学方法检测HO-1蛋白的表达.结果溃疡组、高压氧组、对照组HO-1 mRNA表达量分别为1.063±0.006、1.082±0.007、0.950±0.013,溃疡组、高压氧组与对照组比较,溃疡组与高压氧组比较,差异均有统计学意义(P＜0.01).免疫组织化学结果显示:对照组HO-1表达主要集中在表皮组织;溃疡组和高压氧组表皮和真皮组织中的HO-1表达较正常对照组明显增加,且高压氧组HO-1表达都多于溃疡组.结论糖尿病足部溃疡与其皮肤组织中的HO-1表达呈相关性,糖尿病足部溃疡皮肤组织的抗氧化能力降低.
목적 탐토고압양치료대혈홍소가양매-1(HO-1)재당뇨병족대서피부조직중표체적영향이급HO-1수평여궤양유합적상관성.방법 건강성년웅성Wistar대서45지,체질량250～350 g,안수자표법수궤분위당뇨병족부궤양조(궤양조)、당뇨병족부궤양고압양치료조(고압양조)、정상대조조(대조조),매조15지.3조균이고지사료(88.8％기출사료、1％담고순、10％저유、0.2％담염)사양1개월,제작성2형당뇨병모형,궤양조화고압양조대서재제작성2형당뇨병족궤양모형,고압양조용고압양치료(치료압력0.2 MPa,1차/d,련속치료15 d).연후취3조대서족부피부,채용역전록취합매련반응(PT-PCR)검측피부조직중HO-1 mRNA적표체;채용면역조직화학방법검측HO-1단백적표체.결과 궤양조、고압양조、대조조HO-1 mRNA표체량분별위1.063±0.006、1.082±0.007、0.950±0.013,궤양조、고압양조여대조조비교,궤양조여고압양조비교,차이균유통계학의의(P＜0.01).면역조직화학결과현시:대조조HO-1표체주요집중재표피조직;궤양조화고압양조표피화진피조직중적HO-1표체교정상대조조명현증가,차고압양조HO-1표체도다우궤양조.결론 당뇨병족부궤양여기피부조직중적HO-1표체정상관성,당뇨병족부궤양피부조직적항양화능력강저.
Objective To explore the expression of HO-1 in the dermal tissue of rats with type Ⅱ diabetic foot ulcer and the association between HO-1 level and ulcer healing.Methods Forty-five Wistar male healthy rats with the weight ranging from 250 to 350 g were randomly divided into 3 groups:the diabetic foot ulcer group(the DF group),the diabetic foot ulcer hyperbaric oxygen therapy group (or the DFHBO group or simply the HBO group) and the normal control group (or the NC group),each consisting of 15 animals.The mRNA transcription of HO-1 was detected by RT-PCR.The animals of the 3 groups were all raised with high lipid feed (88.8％ basic feed,1％ cholesterol,10％ lard and 0.2％ bile salt) for a period of one month.Then,the animal model of type Ⅱ diabetes was developed,and the model of type Ⅱ diabetes with diabetic food ulcer was further developed in the animals of the DF group and the DFHBO group.The animals in the DFHBO group were treated with HBO,at a pressure ofO.2 MPa,one session a day,for a duration of 15 days.Then,skin samples were collected from the foot of the animals in the 3 groups.The expression of HO-1 mRNA was detected by PT-PCR and the expression of HO-1 was determined by immunohistochemistry.Results The mRNA expression levels in the DF group,the HBO group and the NC group were respectively 1.063 ± 0.006,1.082±0.007 and 0.950 ± 0.013.Statistical significance could be noted,when comparisons were made between the 3 groups (P ＜0.01),and statistical difference could also be seen,when comparisons were made between the DF group and the HBO group (P ＜ 0.01).Immunohistochemistry detection indicated that HO-1 expression was more significant in epidermal tissue for the NC group.The expression levels of HO-1 both in the epidermal and dermal tissues for the animals of the DF group and the HBO group were more significant,when comparisons were made with that of the NC group,and the HO-1 expression level for the animals in the HBO group was higher than that of the DF group.Conclusions Diabetic foot ulcer was positively correlated with the expression of HO-1 in the dermal tissue.It was possible that antioxidation decreased in the dermal tissue of rats with diabetic foot ulcers.