CAJ | 학술논문

目的通过对大鼠行为学及应激相关指标的观察,探讨高压氧与应激的关系.方法 20只Wistar大鼠按照数字表法随机分为高压氧组和空白对照组,高压氧组给予0.20 MPa高压氧处理,连续治疗3周;对照组单纯喂养,分别在第1、7、14、21天对其进行高架十字迷宫试验,观察大鼠开臂逗留时间,利用酶联免疫吸附测定法测定其血清中促肾上腺皮质激素释放激素、促肾上腺皮质激素、糖皮质激素、一氧化氮合酶、一氧化氮及肿瘤坏死因子-α(TNF-α)含量.结果高压氧处理可明显增加大鼠外周血液中糖皮质激素(glucocorticoids,GCS)的水平,差异具有统计学意义(P＜0.05),但对大鼠高架十字迷宫中的开臂逗留时间、一氧化氮系统及TNF-α的表达无明显影响(P＞0.05).结论高压氧处理符合慢性应激的一般特征,但又不对机体造成明显的不良影响,提示高压氧的作用机制之一是作为一种良性应激来增加机体对应激的耐受能力.
목적 통과대대서행위학급응격상관지표적관찰,탐토고압양여응격적관계.방법 20지Wistar대서안조수자표법수궤분위고압양조화공백대조조,고압양조급여0.20 MPa고압양처리,련속치료3주;대조조단순위양,분별재제1、7、14、21천대기진행고가십자미궁시험,관찰대서개비두류시간,이용매련면역흡부측정법측정기혈청중촉신상선피질격소석방격소、촉신상선피질격소、당피질격소、일양화담합매、일양화담급종류배사인자-α(TNF-α)함량.결과 고압양처리가명현증가대서외주혈액중당피질격소(glucocorticoids,GCS)적수평,차이구유통계학의의(P＜0.05),단대대서고가십자미궁중적개비두류시간、일양화담계통급TNF-α적표체무명현영향(P＞0.05).결론 고압양처리부합만성응격적일반특정,단우불대궤체조성명현적불량영향,제시고압양적작용궤제지일시작위일충량성응격래증가궤체대응격적내수능력.
Objective To investigate the relationship between hyperbaric oxygen (HBO) and stress through observation on rats behavior and stress-related indicators.Methods Twenty Wistar rats were randomly divided into the HBO group and the control group.The HBO group was exposed to HBO at a pressure of 0.20 MPa once a day,for a succession of 21 days,and the control group was left there without HBO treatment.After one week of adaptive training,elevated plus maze test was conducted at days 1,7,14 and 21 to observe openarm (OA) time.The contents of corticotropin-releasing hormone (CRH),adrenocorticotropic hormone (ATCH),glucocorticoids (GCS),nitric oxide synthase (NOS),nitric oxide (NO) and tumor necrosis factorα (TNF-α) in serum of the animals in each group were detected by ELISA (enzyme-linked immunosorbent assay).Results HBO treatment could significantly increase the level of GCS in peripheral blood (P ＞ 0.05),but with no obvious effect on open-arm time (anxiety degree) and the expression levels of nitric oxide and TNF-α.Conclusions HBO treatment was in conformity with the general characteristics of chronic stress,but would not bring about damage to the body,suggesting that one of the mechanisms of HBO might lie in the increased tolerance of the involved tissues to stressors.