中华航海医学与高气压医学杂志
中華航海醫學與高氣壓醫學雜誌
중화항해의학여고기압의학잡지
CHINESE JOURNAL OF NAUTICAL MEDICINE AND HYPERBARIC MEDICINE
2014年
6期
377-380,386
,共5页
崔建华%吴佩锋%高亮%李年华%王琰%李彬%王福领
崔建華%吳珮鋒%高亮%李年華%王琰%李彬%王福領
최건화%오패봉%고량%리년화%왕염%리빈%왕복령
大鼠%高压氧%急性缺氧%超微结构%含水量
大鼠%高壓氧%急性缺氧%超微結構%含水量
대서%고압양%급성결양%초미결구%함수량
Rats%Hyperbaric oxygen%Acute hypoxia%Ultrastructure%Water content
目的 观察高压氧(HBO)预处理后急性缺氧(海拔7 000m)大鼠心、脑、肺超微结构及含水量的变化.方法 雄性健康SD大鼠50只,体质量200~220 g,按数字表法随机分为6组.HBO组(Ⅰ、Ⅱ、Ⅲ)给予HBO预处理7d.HBO Ⅰ组(n=10):HBO预处理后在海拔7 000m低压舱内12h,HBO Ⅱ组(n=10):HBO预处理后在低压舱内24 h,HBO Ⅲ组(n=10):HBO预处理后在低压舱内72 h,对照Ⅰ组(n=6):在低压舱内12 h,对照Ⅱ组(n=7):在低压舱内24 h,对照Ⅲ组(n=7):在低压舱内72 h.各组大鼠在低压舱内快速处死取出额叶脑皮质、心、肺组织标本进行光镜和电镜观察,并取左侧脑和肺组织称湿重,烘干计算脑、肺组织含水量.结果 HBO组脑和肺组织含水量较对照组明显降低,如:HBOⅠ组左脑(74.16±6.88)%、左肺(71.49±7.73)%,对照Ⅰ组左脑(81.58 ±5.27)%、左肺(73.44±5.50)%,差异有统计学意义(P<0.05或P<0.01),急性缺氧72 h内随时间延长,含水量增高.急性缺氧大鼠肺组织结构排列紊乱,肺毛细血管充血明显,肺泡壁明显增厚,部分区域内可见肺泡腔内有粉红色渗出物,出现明显的间质性肺水肿和肺泡性肺水肿;心肌细胞核变形、浊肿、空泡变性及心肌间质水肿;脑核膜模糊不清,核膜表面不平滑.随着缺氧时间的延长组织损伤加重.HBO预处理后肺组织结构排列完整,肺泡壁稍有增厚;心肌除少部分神经元轻度肿胀,其细胞轮廓略不规则,其余基本与正常的细胞结构相同;脑细胞膜及核膜完整,核质较均匀.结论 HBO预处理可预防大鼠急性缺氧引起的脑水肿和肺水肿,对缺氧组织有明显的保护作用.
目的 觀察高壓氧(HBO)預處理後急性缺氧(海拔7 000m)大鼠心、腦、肺超微結構及含水量的變化.方法 雄性健康SD大鼠50隻,體質量200~220 g,按數字錶法隨機分為6組.HBO組(Ⅰ、Ⅱ、Ⅲ)給予HBO預處理7d.HBO Ⅰ組(n=10):HBO預處理後在海拔7 000m低壓艙內12h,HBO Ⅱ組(n=10):HBO預處理後在低壓艙內24 h,HBO Ⅲ組(n=10):HBO預處理後在低壓艙內72 h,對照Ⅰ組(n=6):在低壓艙內12 h,對照Ⅱ組(n=7):在低壓艙內24 h,對照Ⅲ組(n=7):在低壓艙內72 h.各組大鼠在低壓艙內快速處死取齣額葉腦皮質、心、肺組織標本進行光鏡和電鏡觀察,併取左側腦和肺組織稱濕重,烘榦計算腦、肺組織含水量.結果 HBO組腦和肺組織含水量較對照組明顯降低,如:HBOⅠ組左腦(74.16±6.88)%、左肺(71.49±7.73)%,對照Ⅰ組左腦(81.58 ±5.27)%、左肺(73.44±5.50)%,差異有統計學意義(P<0.05或P<0.01),急性缺氧72 h內隨時間延長,含水量增高.急性缺氧大鼠肺組織結構排列紊亂,肺毛細血管充血明顯,肺泡壁明顯增厚,部分區域內可見肺泡腔內有粉紅色滲齣物,齣現明顯的間質性肺水腫和肺泡性肺水腫;心肌細胞覈變形、濁腫、空泡變性及心肌間質水腫;腦覈膜模糊不清,覈膜錶麵不平滑.隨著缺氧時間的延長組織損傷加重.HBO預處理後肺組織結構排列完整,肺泡壁稍有增厚;心肌除少部分神經元輕度腫脹,其細胞輪廓略不規則,其餘基本與正常的細胞結構相同;腦細胞膜及覈膜完整,覈質較均勻.結論 HBO預處理可預防大鼠急性缺氧引起的腦水腫和肺水腫,對缺氧組織有明顯的保護作用.
목적 관찰고압양(HBO)예처리후급성결양(해발7 000m)대서심、뇌、폐초미결구급함수량적변화.방법 웅성건강SD대서50지,체질량200~220 g,안수자표법수궤분위6조.HBO조(Ⅰ、Ⅱ、Ⅲ)급여HBO예처리7d.HBO Ⅰ조(n=10):HBO예처리후재해발7 000m저압창내12h,HBO Ⅱ조(n=10):HBO예처리후재저압창내24 h,HBO Ⅲ조(n=10):HBO예처리후재저압창내72 h,대조Ⅰ조(n=6):재저압창내12 h,대조Ⅱ조(n=7):재저압창내24 h,대조Ⅲ조(n=7):재저압창내72 h.각조대서재저압창내쾌속처사취출액협뇌피질、심、폐조직표본진행광경화전경관찰,병취좌측뇌화폐조직칭습중,홍간계산뇌、폐조직함수량.결과 HBO조뇌화폐조직함수량교대조조명현강저,여:HBOⅠ조좌뇌(74.16±6.88)%、좌폐(71.49±7.73)%,대조Ⅰ조좌뇌(81.58 ±5.27)%、좌폐(73.44±5.50)%,차이유통계학의의(P<0.05혹P<0.01),급성결양72 h내수시간연장,함수량증고.급성결양대서폐조직결구배렬문란,폐모세혈관충혈명현,폐포벽명현증후,부분구역내가견폐포강내유분홍색삼출물,출현명현적간질성폐수종화폐포성폐수종;심기세포핵변형、탁종、공포변성급심기간질수종;뇌핵막모호불청,핵막표면불평활.수착결양시간적연장조직손상가중.HBO예처리후폐조직결구배렬완정,폐포벽초유증후;심기제소부분신경원경도종창,기세포륜곽략불규칙,기여기본여정상적세포결구상동;뇌세포막급핵막완정,핵질교균균.결론 HBO예처리가예방대서급성결양인기적뇌수종화폐수종,대결양조직유명현적보호작용.
Objective To observe the effects of hyperbaric oxygen (HBO) pretreatment on the ultrastructure of heart,brain and lung induced by acute hypoxia (altitude of 7 000 m) in rats and changes of water content in the tissues involved.Methods Fifty SD rats were randomly divided into 6 groups:3 HBO groups (the HBO group 1,the HBO group 2,and the HBO group 3) and 3 control groups (the control group 1,the control group 2,and the control group 3).The HBO groups were all pretreated with HBO for 7 days.The HBO group 1 (n =10),HBO group 2 (n =10),and HBO group 3 (n =10) were exposed to an altitude of 7 000 m in the hypobaric chamber for durations of 12 h,24 h,and 72 h respectively,following HBO pretreatment.The control group 1 (n =6),control group 2(n =7) and the control group 3 (n =7) stayed in the hypobaric chamber for durations of 12 h,24 h,and 72 h respectively.The rats in all the groups were quickly sacrificed in the hypobaric chamber and frontal cortex,heart and lung samples were collected for optical microscopy and electron microscopy.Wet weight of the left brain and lung were measured and dried to calculate water contents of the tissues.Results Water contents in the tissues of the rats in the HBO groups were all decreased markedly than those in the control groups,with statistical significance (P < 0.05 or 0.01).Tissue water contents increased with the prolonging of exposure time during acute hypoxia within 72 h.In rats with acute hypoxia,lung tissue structure was in disorder,pulmonary capillary vessels were congested significantly,alveolar wall was thickened,pink alveolar exudation was clearly visible in some areas,and significant interstitial pulmonary edema and alveolar pulmonary edema were also present.Myocardial nucleuses were deformed with cloudy swelling,vacuolar degeneration and myocardial interstitial edema.Brain nuclear membrane was indistinct with an unsmooth surface.Tissue injury aggravated with prolonged hypoxia.However,for the animals in the HBO pretreatment groups,things were different:lung tissue structure was in proper order,alveolar wall was slightly thickened,myocardial cells were slightly swollen with the exception of a few neurons,cell circumscription was slightly irregular,brain cell membranes and nucleolemma were intact with uniform nucleoplasm.Conclusions HBO pretreatment could prevent cerebral edema and lung edema induced by acute hypoxia,and could produce good protective effect on the tissues affected by hypoxia.