CAJ | 학술논문

目的探讨高压氧(hyperbaric oxygen,HBO)治疗对脑外伤大鼠认知功能部分指标的影响.方法 36只雄性SD大鼠按数字表法随机分为HBO组、常压空气治疗组及假手术组,每组各12只.HBO组和常压空气治疗组均采用Feeney自由落体法制作脑外伤模型,HBO组每天进行HBO治疗;常压空气治疗组置于常压空气中;假手术组仅暴露出硬脑膜不予打击.运用Morris水迷宫测试认知功能;1H-MRS观察HBO治疗大鼠脑外伤后海马区N-乙酰基天门冬氨酸(NAA)/肌酸(Cr)及N-乙酰基天门冬氨酸/胆碱(Ch)值的变化;并运用实时定量PCR观察HBO治疗后损伤侧海马脑源性神经营养因子(BDNF)、神经生长因子(NGF)的变化情况.结果 HBO组在HBO治疗后7d平均逃避潜伏期下降[(35.1±2.8)s],同时穿越平台次数增多(4.00±0.42),与常压空气治疗组[(59.4±5.0)s;2.44±0.45]相比差异均有统计学意义(P＜0.05).术后7 d HBO组经HBO治疗后NAA/Cr值(1.18±0.11)与常压空气治疗组(0.85±0.03)相比差异有统计学意义(P＜0.05);术后8h和7 d HBO组NAA/Ch值(1.14±0.10、1.33±0.17)与常压空气治疗组比较,(0.79±0.07、0.86±0.08)相比差异有统计学意义(P＜0.05).与常压空气治疗组[HBO组3、7 d NGF(1.27±0.03、1.31±0.08)和BDNF mRNA(2.82±0.20、1.07±0.19)]表达差异均有统计学意义(P＜0.05).结论 HBO治疗可以改善脑外伤大鼠认知功能,其机制可能与海马区NAA/Cr及NAA/Ch值上调、NGF和BDNF表达上升有关.
목적 탐토고압양(hyperbaric oxygen,HBO)치료대뇌외상대서인지공능부분지표적영향.방법 36지웅성SD대서안수자표법수궤분위HBO조、상압공기치료조급가수술조,매조각12지.HBO조화상압공기치료조균채용Feeney자유락체법제작뇌외상모형,HBO조매천진행HBO치료;상압공기치료조치우상압공기중;가수술조부폭로출경뇌막불여타격.운용Morris수미궁측시인지공능;1H-MRS관찰HBO치료대서뇌외상후해마구N-을선기천문동안산(NAA)/기산(Cr)급N-을선기천문동안산/담감(Ch)치적변화;병운용실시정량PCR관찰HBO치료후손상측해마뇌원성신경영양인자(BDNF)、신경생장인자(NGF)적변화정황.결과 HBO조재HBO치료후7d평균도피잠복기하강[(35.1±2.8)s],동시천월평태차수증다(4.00±0.42),여상압공기치료조[(59.4±5.0)s;2.44±0.45]상비차이균유통계학의의(P＜0.05).술후7 d HBO조경HBO치료후NAA/Cr치(1.18±0.11)여상압공기치료조(0.85±0.03)상비차이유통계학의의(P＜0.05);술후8h화7 d HBO조NAA/Ch치(1.14±0.10、1.33±0.17)여상압공기치료조비교,(0.79±0.07、0.86±0.08)상비차이유통계학의의(P＜0.05).여상압공기치료조[HBO조3、7 d NGF(1.27±0.03、1.31±0.08)화BDNF mRNA(2.82±0.20、1.07±0.19)]표체차이균유통계학의의(P＜0.05).결론 HBO치료가이개선뇌외상대서인지공능,기궤제가능여해마구NAA/Cr급NAA/Ch치상조、NGF화BDNF표체상승유관.
Objective To investigate the effect of hyperbaric oxygen (HBO) on cognitive function in rats following traumatic brain injury.Methods Thirty-six male Sprague-Dawley rats were randomly divided into the HBO group,normobaric air therapy group and the sham surgical group,each consisting of 12 animals.Traumatic brain injury model was established by using Feeney's free falling in the animals of the HBO group and the normobaric air therapy group.The HBO group received HBO therapy every day and the normobaric air therapy group was left there in normal atmospheric air.The animals in the sham surgical group just exposed dura mater,but without free fall impact.Cognitive function was assessed by using Morris water maze.Dynamic changes in N-acetylaspartate/creatine (NAA/Cr) levels in hippocampus and NAA/choline (NAA/Ch) levels were closely observed with H-MRS following treatment of traumatic brain injury with HBO,and real-time PCR was used to evaluate changes in the levels of nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF) mRNA in the ipsilateral hippocampus.Results Seven days after HBO therapy,escape latency decreased [(35.1 ± 2.8) s] and number of times crossing the platform increased for the animals in the HBO group.Statistical significance could be noted,when they were compared with those of the normobaric air therapy group[(59.4 ±5.0) s;2.44 ±0.45] (P ＜0.05).NAA/Cr levels in the HBO group were significantly increased (1.18 ± 0.11) 7 days after HBO therapy,and statistical significance could also be seen,as compared with those of the normobaric air therapy group(0.85 ± 0.03) (P ＜ 0.05).Compared with those of the normobaric air therapy group (8 h:0.79 ± 0.07 ; 7 d:0.86 ± 0.08),NAA/Ch levels in the ipsilateral hippocampus of the HBO group (8 h:1.14 ± 0.10; 7 d:1.33 ± 0.17) displayed statistical significance at h 8 and d 7 (P＜0.05).The levels of NGF(3 d:1.27 ±0.03; 7 d:1.31 ±0.08)and BDNF mRNA for the HBO group (3 d:2.82 ±0.20; 7 d:1.07 ±0.08)and BDNF mRNA(3 d:2.82±0.20; 7 d:1.07 ±0.19)were increased at d 3 and d 7,as compared with those of the normobaric air therapy group(NGF 3 d:0.66 ±0.10,7 d:0.73 ±0.07); (BDNF 3 d:1.51 ±0.11; 7 d:0.65±0.09),with statistical significances (P ＜ 0.05).Conclusions HBO therapy could promote cognitive function in rats with traumatic brain injury,the possible mechanism might be associated with the increases in NAA/Cr and NAA/Ch levels and the levels of NGF and BDNF mRNA in hippocampus.