CAJ | 학술논문

目的探讨法舒地尔对阻塞性黄疸大鼠肝缺血再灌注损伤的影响.方法成熟SD大鼠160只随机分成实验组(A组)及对照组(B组)两大组,两组再分别分为4个亚组,分别为假手术组(A1、B1组)、阻塞性黄疸组(A2、B2组)、肝缺血再灌注组(A3、B3组)及阻塞性黄疸+肝缺血再灌注组(A4、B4组).A2、B2组采用双重结扎切断胆总管的方法建立模型,A3、B3组采用阻断肝门部血管30 min后再灌注的方法建立模型.A4、B4组于建立阻塞性黄疸模型1周后再次建立缺血再灌注模型.A组于缺血前30 min腹腔内注射法舒地尔10 mg/kg,B组对应注射等量生理盐水.两组分别于再灌注0h、1h、2h、6h取材,检测血清肝功能改变及血清内皮素1水平.采用SPSS软件对重复测量结果进行统计分析,光镜下观察肝脏病理学改变.结果实验组各时点血清转氨酶水平、血清内皮素1水平均显著低于对照组(P＜0.05),实验组各时点光镜下肝脏病理组织学损伤也较对照组明显减轻.结论法舒地尔对阻塞性黄疸大鼠的肝缺血再灌注损伤具有保护作用.
목적 탐토법서지이대조새성황달대서간결혈재관주손상적영향.방법 성숙SD대서160지수궤분성실험조(A조)급대조조(B조)량대조,량조재분별분위4개아조,분별위가수술조(A1、B1조)、조새성황달조(A2、B2조)、간결혈재관주조(A3、B3조)급조새성황달+간결혈재관주조(A4、B4조).A2、B2조채용쌍중결찰절단담총관적방법건립모형,A3、B3조채용조단간문부혈관30 min후재관주적방법건립모형.A4、B4조우건립조새성황달모형1주후재차건립결혈재관주모형.A조우결혈전30 min복강내주사법서지이10 mg/kg,B조대응주사등량생리염수.량조분별우재관주0h、1h、2h、6h취재,검측혈청간공능개변급혈청내피소1수평.채용SPSS연건대중복측량결과진행통계분석,광경하관찰간장병이학개변.결과 실험조각시점혈청전안매수평、혈청내피소1수평균현저저우대조조(P＜0.05),실험조각시점광경하간장병리조직학손상야교대조조명현감경.결론 법서지이대조새성황달대서적간결혈재관주손상구유보호작용.
Objetive To investigate the effect of Fasudil on the hepatic ischemia repeffusion injury on rats of obstructive jaundice.Methods 160 mature SD rats were randomly assigned into 2 large groups,namely experimental group (Group A) and control group (Group B).While each large group was subdivided into 4 subgroups,including sham operation subgroups(Group A1 and B1),obstructive jaundice model subgroups(Group A2 and B2),ischemia reperfusion model subgroups (Group A3 and B3)and obstructive jaundice plus ischemia reperfusion model subgroups (Group A4 and B4).In Group A2 and B2,obstructive jaundice models were made on rats by double ligating and cutting the common bile ducts.In Group A3 and B3,the ischemia reperfusion injury models were built by blocking the hepatic portal vessel for 30 minutes before reperfusion.In Group A4 and B4,the ischemia reperfusion injury models were built in 1 week after building the obstructive jaundice models.For Group A,10 mg/kg fasudil was intraperitoneally injected at 30 minutes before ischemia.For Group B,equal amount of physiological saline was injected.Samples were gained on zero hour,1 hour,2 hour and 6 hour post reperfusion.The serum indexes for liver function and serum Endothelin 1 level were tested and analyzed through SPSS.Pathological changes of liver were viewed correspondingly.Results Compared to control groups (Group Bs),experimental groups (Groups As) have better liver function,lower Endothelin 1 level (P ＜ 0.05),as well as less severe microscopic histological damage.Conclusion Fasudil can effectively protect rats of obstructive jaundice from hepatic ischemia reperfusion injury.