中华超声影像学杂志
中華超聲影像學雜誌
중화초성영상학잡지
CHINESE JOURNAL OF ULTRASONOGRAPHY
2014年
12期
1075-1078
,共4页
谢佳佳%杨慧%李瑜辉%宾建平
謝佳佳%楊慧%李瑜輝%賓建平
사가가%양혜%리유휘%빈건평
超声检查%分子显像%缺血%新生血管化,生理性%血管内皮生长因子受体2
超聲檢查%分子顯像%缺血%新生血管化,生理性%血管內皮生長因子受體2
초성검사%분자현상%결혈%신생혈관화,생이성%혈관내피생장인자수체2
Ultrasonography%Molecular imaging%Ischemia%Neovascularization,physiologic%Vascular endothelial growth factor receptor-2
目的 探讨应用携血管内皮生长因子受体-2(vascular endothelial growth factor receptor-2,VEGFR-2)分子探针的超声分子成像评价小鼠下肢缺血性血管新生的可行性.方法 12只实验小鼠结扎一侧下肢股动脉制备下肢缺血模型,另一侧下肢作为对照组.术后第7d所有小鼠均随机先后间隔30 min经尾静脉注入携抗小鼠VEGFR-2单抗的靶向微泡(MBVEGFR-2)和携同型抗体的对照微泡(MBIso),并于8min后行对比超声检查,测量双侧下肢的显影强度(video intensity,VI),最后处死小鼠取下肢骨骼肌行免疫组化检查.结果 对比超声图像显示MBVEGFR-2组缺血下肢可见明显的超声显影,VI值高达(25.6±4.3)U,而在MBIso组缺血下肢仅可见轻度的超声显影,VI值为(6.7±1.6)U,两者之间的差异有统计学意义(P＜0.05).但无论MBVEGFR-2还是MBISO,缺血下肢VI值均显著高于非缺血下肢VI值(P＜0.05).两组微泡在非缺血下肢的VI值差异无统计学意义(P＞0.05).免疫组化检查显示缺血下肢血管内皮表达大量的VEGFR-2,而非缺血下肢未见VEGFR 2表达.结论 应用VEGFR-2分子探针的超声分子成像可有效评价下肢缺血性血管新生,将为评价治疗性或相关的血管新生提供新的技术手段.
目的 探討應用攜血管內皮生長因子受體-2(vascular endothelial growth factor receptor-2,VEGFR-2)分子探針的超聲分子成像評價小鼠下肢缺血性血管新生的可行性.方法 12隻實驗小鼠結扎一側下肢股動脈製備下肢缺血模型,另一側下肢作為對照組.術後第7d所有小鼠均隨機先後間隔30 min經尾靜脈註入攜抗小鼠VEGFR-2單抗的靶嚮微泡(MBVEGFR-2)和攜同型抗體的對照微泡(MBIso),併于8min後行對比超聲檢查,測量雙側下肢的顯影彊度(video intensity,VI),最後處死小鼠取下肢骨骼肌行免疫組化檢查.結果 對比超聲圖像顯示MBVEGFR-2組缺血下肢可見明顯的超聲顯影,VI值高達(25.6±4.3)U,而在MBIso組缺血下肢僅可見輕度的超聲顯影,VI值為(6.7±1.6)U,兩者之間的差異有統計學意義(P＜0.05).但無論MBVEGFR-2還是MBISO,缺血下肢VI值均顯著高于非缺血下肢VI值(P＜0.05).兩組微泡在非缺血下肢的VI值差異無統計學意義(P＞0.05).免疫組化檢查顯示缺血下肢血管內皮錶達大量的VEGFR-2,而非缺血下肢未見VEGFR 2錶達.結論 應用VEGFR-2分子探針的超聲分子成像可有效評價下肢缺血性血管新生,將為評價治療性或相關的血管新生提供新的技術手段.
목적 탐토응용휴혈관내피생장인자수체-2(vascular endothelial growth factor receptor-2,VEGFR-2)분자탐침적초성분자성상평개소서하지결혈성혈관신생적가행성.방법 12지실험소서결찰일측하지고동맥제비하지결혈모형,령일측하지작위대조조.술후제7d소유소서균수궤선후간격30 min경미정맥주입휴항소서VEGFR-2단항적파향미포(MBVEGFR-2)화휴동형항체적대조미포(MBIso),병우8min후행대비초성검사,측량쌍측하지적현영강도(video intensity,VI),최후처사소서취하지골격기행면역조화검사.결과 대비초성도상현시MBVEGFR-2조결혈하지가견명현적초성현영,VI치고체(25.6±4.3)U,이재MBIso조결혈하지부가견경도적초성현영,VI치위(6.7±1.6)U,량자지간적차이유통계학의의(P＜0.05).단무론MBVEGFR-2환시MBISO,결혈하지VI치균현저고우비결혈하지VI치(P＜0.05).량조미포재비결혈하지적VI치차이무통계학의의(P＞0.05).면역조화검사현시결혈하지혈관내피표체대량적VEGFR-2,이비결혈하지미견VEGFR 2표체.결론 응용VEGFR-2분자탐침적초성분자성상가유효평개하지결혈성혈관신생,장위평개치료성혹상관적혈관신생제공신적기술수단.
Objective To explore the feasibility of evaluation of mice hind limb ischemia-mediated angiogenesis with ultrasound molecular imaging using molecular probes targeted to angiogenesis endothelial marker VEGFR-2.Methods A mice model of unilateral hind-limb ischemia was induced by femoral artery excision in 12 experimental mice.Ultrasound molecular imaging of the ischemia and contralateral non-ischemia hind-limbs was performed in all mice on day 7 after surgery at 8 minutes after intravenous injection of either VEGFR-2 targeting microbubbles or isotype control microbubbles in random with 30 min interval,and the video intensity (VI) was measured.Following ultrasound imaging,the hind-limb was harvested for immunohistochemical analysis.Results As expected,VI in the ischemia hind-limb was significantly higher (P ＜0.05) for MBvEGFR-2 [(25.6 ± 4.3)U] as compared with MBIso[(6.7 ± 1.6)U].However,the ultrasound signal in the non-ischemia hind-limb was low for both MBvEGFR-2 [4.4 ± 1.5)U] and MBIso [(4.6 ± 1.6)U].A marked endothelial VEGFR-2 expression in ischemia hind-limb was confirmed by immunohistochemistry.Conclusions Ultrasound molecular imaging using molecular probes targeted to angiogenesis endothelial VEGFR-2 can effectively evaluate ischemia-mediated angiogenesis.
