CAJ | 학술논문

目的探讨术后疼痛大鼠切口组织Toll样受体4(TLR4)及其下游细胞因子IL-1β、IL-6和TNF-αmRNA表达的变化. 方法健康成年雄性SD大鼠74只,体重200～250 g,建立大鼠术后疼痛模型.于术前1d、术后0.5,1,2,6,12h及1,2,3,5,7d测定术侧与非术侧机械缩足反射阈值(PMWT);于术前1d、术后2,8h、1,2,3,5,7d采用荧光定量PCR法检测皮肤切口组织TLR4、IL-1β、IL-6和TNF-α mRNA的表达. 结果与术前比较,大鼠术侧术后0.5 h～5 dPMWT降低,术后2h术侧切口组织TLR4mRNA表达下调,之后逐渐升高,IL-1β、IL-6和TNF-αmRNA于术后2,8h、1,2,3,5d表达上调(P＜0.05),非术侧PMWT差异无统计学意义(P＞0.05);术侧PMWT术后6h最低,之后逐渐升高,术侧切口组织TLR4mRNA术后2d表达水平最高,IL-1β、IL-6和TNF-α mRNA分别于术后2h、1d、3d表达水平最高(P＜0.05).TLR4、IL-1β、IL-6和TNF-αmRNA表达水平与术侧PMWT呈负相关(r=-0.501,-0.743,-0.893,-0.657,P＜0.05),IL-1β、IL-6和TNF-αmRNA与TLR4 mRNA表达水平呈正相关(r =0.764,0.283,0.667,P＜0.05). 结论术后疼痛大鼠切口组织TLR4及其下游炎性因子IL-1β、IL-6、TNF-α mRNA表达上调,该变化可能参与了术后疼痛的产生和维持.
목적 탐토술후동통대서절구조직Toll양수체4(TLR4)급기하유세포인자IL-1β、IL-6화TNF-αmRNA표체적변화. 방법 건강성년웅성SD대서74지,체중200～250 g,건립대서술후동통모형.우술전1d、술후0.5,1,2,6,12h급1,2,3,5,7d측정술측여비술측궤계축족반사역치(PMWT);우술전1d、술후2,8h、1,2,3,5,7d채용형광정량PCR법검측피부절구조직TLR4、IL-1β、IL-6화TNF-α mRNA적표체. 결과 여술전비교,대서술측술후0.5 h～5 dPMWT강저,술후2h술측절구조직TLR4mRNA표체하조,지후축점승고,IL-1β、IL-6화TNF-αmRNA우술후2,8h、1,2,3,5d표체상조(P＜0.05),비술측PMWT차이무통계학의의(P＞0.05);술측PMWT술후6h최저,지후축점승고,술측절구조직TLR4mRNA술후2d표체수평최고,IL-1β、IL-6화TNF-α mRNA분별우술후2h、1d、3d표체수평최고(P＜0.05).TLR4、IL-1β、IL-6화TNF-αmRNA표체수평여술측PMWT정부상관(r=-0.501,-0.743,-0.893,-0.657,P＜0.05),IL-1β、IL-6화TNF-αmRNA여TLR4 mRNA표체수평정정상관(r =0.764,0.283,0.667,P＜0.05). 결론 술후동통대서절구조직TLR4급기하유염성인자IL-1β、IL-6、TNF-α mRNA표체상조,해변화가능삼여료술후동통적산생화유지.
Objective To evaluate the changes in mRNA expressions of Toll-like receptor 4 (TLR4) and its down-stream cytokines IL-1β,IL-6 and TNF-a in incisional tissues from a rat with postoperative pain.Methods Incisional pain was induced in 74 male adult SD rats weighing 200-250 g.Paw mechanical withdrawal threshold (PMWT) around the wound on the operated and nonoperated sides was measured at 1 day before operation and at 0.5,1,2,6 and 12 hours as well as 1,2,3,5 and 7 days after operation.Skin incisional tissues were removed for determination of mRNA expressions of TLR4,IL-1β,IL-6 and TNF-a using real-time quantitative PCR at 1 day before operation and at 2 and 8 hours as well as 1,2,3,5 and 7 days after operation.Results Compared with the baseline value before operation,PMWT on the operated side was significantly decreased at 0.5 hours-5 days after operation,mRNA expression of TLR4 around the wounds on the operated side was down-regulated at 2 hours after operation followed by a gradual increase,mRNA expressions of IL-1β,IL-6 and TNF-α on the operative side were up-regulated at 2 and 8 hours as well as 1,2,3 and 5 days after operation (P ＜ 0.05),but no significant changes were found in PMWT and mRNA expressions of TLR4,IL-1β,IL-6 and TNF-α on the non-operated side(P ＞ 0.05).PMWT on the operated side was lowest at 6 hours after operation followed by the gradual increase,mRNA expression of TLR4 on the operated side peaked at 2 days after operation,and mRNA expressions of IL-1β,IL-6 and TNF-a respectively peaked at 2 hours,1 day and 3 days after operation (P ＜ 0.05).mRNA expressions of TLR4,IL-1β,IL-6 and TNF-a were negatively correlated with PMWT on the operative side (r =-0.501,-0.743,-0.893,-0.657,P ＜ 0.05),and mRNA expressions of IL-1 β,IL-6 and TNF-a were positively correlated with the level of TLR4 mRNA(r=0.764,0.283,0.667,P＜0.05).Conclusion mRNA expressions of TLR4 and its down-stream cytokines IL-1 β,IL-6 and TNF-a in skin incisional tissues are up-regulated,which may be involved in the development and maintenance of postoperative pain.