目的 探讨银杏叶片对砷暴露大鼠免疫毒性的干预作用,为砷中毒的预防和治疗提供实验依据.方法 40只Wistar大鼠,使用随机数字表法,按照体质量随机分为4组:对照组、染砷组、银杏叶片治疗组、自然恢复组,每组10只,对照组大鼠自由摄取正常普通饲料90d;其他组大鼠自由摄取含砷饲料90 d;自然恢复组大鼠自由摄取含砷(100 mg/kg)饲料90d后,再给予正常普通饲料饲养45 d;银杏叶片治疗组大鼠自由摄取含砷饲料90 d后,再给予银杏叶片溶液(25 mg/kg·bw)灌胃,6d/周,治疗45 d,治疗期间喂饲正常普通饲料.然后收集大鼠24 h尿液,测定大鼠尿砷含量,采集大鼠静脉血,检测T淋巴细胞亚群CD3+、CD4+、CD8+阳性率,血清抗体IgG、IgM、IgA及补体C3、C4含量.结果 染砷组、自然恢复组、银杏叶片治疗组大鼠尿砷几何均数(2.991、0.421、0.334 mg/g)均高于对照组[0.141 mg/g,P均＜0.05].自然恢复组、银杏叶片治疗组大鼠尿砷均低于染砷组(P均＜ 0.05),自然恢复组大鼠尿砷含量与银杏叶片治疗组比较,差异无统计学意义(P＞0.05).染砷组CD3+、CD4+细胞阳性率[(31.31±7.73)％、(25.94±12.49)％]、CD4+/CD8+比值(0.91±0.50)均低于对照组[(60.83±17.64)％、(42.11±12.92)％、1.80±0.88,P均＜0.05],血清中补体C4含量[(81.18±13.23) mg/L]高于对照组[(64.23±6.97) mg/L,P＜0.01];自然恢复组CD3+、CD4+细胞阳性率[(32.35±19.84)％、(28.00±16.10)％]、CD4+/CD8+比值(0.98±0.29)、血清IgG、IgA、IgM、C3、C4含量[(897.23±23.55)、(1032.72±0.35)、(443.65±1.02)、(557.33±39.86)、(76.92±17.99)mg/L]与染砷组[(31.31±7.73)％、(25.94±12.49)％,0.91±0.50,(917.02±15.96)、(1032.96±1.32)、(444.43±1.82)、(560.15±39.57)、(81.18±13.23)mg/L]比较差异均无统计学意义(P均＞ 0.05);银杏叶片治疗组CD3+、CD4+细胞阳性率[(54.00±19.31)％、(39.93±8.63)％]均高于染砷组[(31.31±7.73)％、(25.94±12.49)％,P均＜0.05],血清中补体C4含量[(66.99±5.66)mg/L]低于染砷组[(64.23±6.97) mg/L,P＜0.05];染砷组、自然恢复组、银杏叶片组CD8+细胞阳性率以及血清IgG、IgA、IgM、C3含量[(31.38±11.40)％,(917.02±1596)、(1 032.96±1.32)、(444.43±1.82)、(560.15±39.57)mg/L;(27.90±13.22)％,(897.23±23.55)、(1 032.72±0.35)、(443.65±1.02)、(557.33±39.86)mg/L; (28.21±8.42)％,(905.83±24.16)、(1 032.10±0.80)、(442.65±1.32)、(554.41±41.21)mg/L]与对照组[(27.22±13.00)％,(903.02±14.69)、(1 032.04±0.64)、(443.41±0.93)、(536.61±32.92)mg/L]比较差异均无统计学意义(P均＞ 0.05).结论 砷暴露可引起大鼠体内细胞免疫功能紊乱;停止砷暴露后,自然恢复效果欠佳;银杏叶片可提高砷中毒大鼠的免疫功能.
目的 探討銀杏葉片對砷暴露大鼠免疫毒性的榦預作用,為砷中毒的預防和治療提供實驗依據.方法 40隻Wistar大鼠,使用隨機數字錶法,按照體質量隨機分為4組:對照組、染砷組、銀杏葉片治療組、自然恢複組,每組10隻,對照組大鼠自由攝取正常普通飼料90d;其他組大鼠自由攝取含砷飼料90 d;自然恢複組大鼠自由攝取含砷(100 mg/kg)飼料90d後,再給予正常普通飼料飼養45 d;銀杏葉片治療組大鼠自由攝取含砷飼料90 d後,再給予銀杏葉片溶液(25 mg/kg·bw)灌胃,6d/週,治療45 d,治療期間餵飼正常普通飼料.然後收集大鼠24 h尿液,測定大鼠尿砷含量,採集大鼠靜脈血,檢測T淋巴細胞亞群CD3+、CD4+、CD8+暘性率,血清抗體IgG、IgM、IgA及補體C3、C4含量.結果 染砷組、自然恢複組、銀杏葉片治療組大鼠尿砷幾何均數(2.991、0.421、0.334 mg/g)均高于對照組[0.141 mg/g,P均＜0.05].自然恢複組、銀杏葉片治療組大鼠尿砷均低于染砷組(P均＜ 0.05),自然恢複組大鼠尿砷含量與銀杏葉片治療組比較,差異無統計學意義(P＞0.05).染砷組CD3+、CD4+細胞暘性率[(31.31±7.73)％、(25.94±12.49)％]、CD4+/CD8+比值(0.91±0.50)均低于對照組[(60.83±17.64)％、(42.11±12.92)％、1.80±0.88,P均＜0.05],血清中補體C4含量[(81.18±13.23) mg/L]高于對照組[(64.23±6.97) mg/L,P＜0.01];自然恢複組CD3+、CD4+細胞暘性率[(32.35±19.84)％、(28.00±16.10)％]、CD4+/CD8+比值(0.98±0.29)、血清IgG、IgA、IgM、C3、C4含量[(897.23±23.55)、(1032.72±0.35)、(443.65±1.02)、(557.33±39.86)、(76.92±17.99)mg/L]與染砷組[(31.31±7.73)％、(25.94±12.49)％,0.91±0.50,(917.02±15.96)、(1032.96±1.32)、(444.43±1.82)、(560.15±39.57)、(81.18±13.23)mg/L]比較差異均無統計學意義(P均＞ 0.05);銀杏葉片治療組CD3+、CD4+細胞暘性率[(54.00±19.31)％、(39.93±8.63)％]均高于染砷組[(31.31±7.73)％、(25.94±12.49)％,P均＜0.05],血清中補體C4含量[(66.99±5.66)mg/L]低于染砷組[(64.23±6.97) mg/L,P＜0.05];染砷組、自然恢複組、銀杏葉片組CD8+細胞暘性率以及血清IgG、IgA、IgM、C3含量[(31.38±11.40)％,(917.02±1596)、(1 032.96±1.32)、(444.43±1.82)、(560.15±39.57)mg/L;(27.90±13.22)％,(897.23±23.55)、(1 032.72±0.35)、(443.65±1.02)、(557.33±39.86)mg/L; (28.21±8.42)％,(905.83±24.16)、(1 032.10±0.80)、(442.65±1.32)、(554.41±41.21)mg/L]與對照組[(27.22±13.00)％,(903.02±14.69)、(1 032.04±0.64)、(443.41±0.93)、(536.61±32.92)mg/L]比較差異均無統計學意義(P均＞ 0.05).結論 砷暴露可引起大鼠體內細胞免疫功能紊亂;停止砷暴露後,自然恢複效果欠佳;銀杏葉片可提高砷中毒大鼠的免疫功能.
목적 탐토은행협편대신폭로대서면역독성적간예작용,위신중독적예방화치료제공실험의거.방법 40지Wistar대서,사용수궤수자표법,안조체질량수궤분위4조:대조조、염신조、은행협편치료조、자연회복조,매조10지,대조조대서자유섭취정상보통사료90d;기타조대서자유섭취함신사료90 d;자연회복조대서자유섭취함신(100 mg/kg)사료90d후,재급여정상보통사료사양45 d;은행협편치료조대서자유섭취함신사료90 d후,재급여은행협편용액(25 mg/kg·bw)관위,6d/주,치료45 d,치료기간위사정상보통사료.연후수집대서24 h뇨액,측정대서뇨신함량,채집대서정맥혈,검측T림파세포아군CD3+、CD4+、CD8+양성솔,혈청항체IgG、IgM、IgA급보체C3、C4함량.결과 염신조、자연회복조、은행협편치료조대서뇨신궤하균수(2.991、0.421、0.334 mg/g)균고우대조조[0.141 mg/g,P균＜0.05].자연회복조、은행협편치료조대서뇨신균저우염신조(P균＜ 0.05),자연회복조대서뇨신함량여은행협편치료조비교,차이무통계학의의(P＞0.05).염신조CD3+、CD4+세포양성솔[(31.31±7.73)％、(25.94±12.49)％]、CD4+/CD8+비치(0.91±0.50)균저우대조조[(60.83±17.64)％、(42.11±12.92)％、1.80±0.88,P균＜0.05],혈청중보체C4함량[(81.18±13.23) mg/L]고우대조조[(64.23±6.97) mg/L,P＜0.01];자연회복조CD3+、CD4+세포양성솔[(32.35±19.84)％、(28.00±16.10)％]、CD4+/CD8+비치(0.98±0.29)、혈청IgG、IgA、IgM、C3、C4함량[(897.23±23.55)、(1032.72±0.35)、(443.65±1.02)、(557.33±39.86)、(76.92±17.99)mg/L]여염신조[(31.31±7.73)％、(25.94±12.49)％,0.91±0.50,(917.02±15.96)、(1032.96±1.32)、(444.43±1.82)、(560.15±39.57)、(81.18±13.23)mg/L]비교차이균무통계학의의(P균＞ 0.05);은행협편치료조CD3+、CD4+세포양성솔[(54.00±19.31)％、(39.93±8.63)％]균고우염신조[(31.31±7.73)％、(25.94±12.49)％,P균＜0.05],혈청중보체C4함량[(66.99±5.66)mg/L]저우염신조[(64.23±6.97) mg/L,P＜0.05];염신조、자연회복조、은행협편조CD8+세포양성솔이급혈청IgG、IgA、IgM、C3함량[(31.38±11.40)％,(917.02±1596)、(1 032.96±1.32)、(444.43±1.82)、(560.15±39.57)mg/L;(27.90±13.22)％,(897.23±23.55)、(1 032.72±0.35)、(443.65±1.02)、(557.33±39.86)mg/L; (28.21±8.42)％,(905.83±24.16)、(1 032.10±0.80)、(442.65±1.32)、(554.41±41.21)mg/L]여대조조[(27.22±13.00)％,(903.02±14.69)、(1 032.04±0.64)、(443.41±0.93)、(536.61±32.92)mg/L]비교차이균무통계학의의(P균＞ 0.05).결론 신폭로가인기대서체내세포면역공능문란;정지신폭로후,자연회복효과흠가;은행협편가제고신중독대서적면역공능.
Objective To explore the effects of Gingko biloba on immune function of rats exposed to arsenic,and to provide experimental evidence for prevention and treatment of arsenic poisoning.Methods By using a random number table,40 Wistar rats were randomly divided into four groups according to body weight,10 rats in each group,including the control,arsenic exposure,Ginkgo biloba treatment and natural recovery groups.The control group rats with normal diet ad libitum 90 d; other groups were freely given feed containing arsenic,90 d; the natural recovery group rats were freely given arsenic (100 mg/kg) feed 90 d,and then given a normal diet for 45 d; Ginkgo biloba treatment rats ingested arsenic feed 90 d,and then give Ginkgo biloba solution(25 mg/kg·bw) orally,6 d/week,treatment 45 d,fed normal normal diet during treatment.24 h urine of rats were collected to measure the content of arsenic in urine.The blood of rats in heart were collected,to determine the positive rates of CD3+,CD4+,CD8+ cells and antibodies (IgG,IgM,IgA),complements (C3,C4).Results Urinary arsenic levels of rats in arsenic exposure,natural recovery,Ginkgo biloba treatment groups (2.991,0.421,0.334 mg/g) were higher than that of control group (0.141 mg/g,P ＜ 0.05).The content of urine arsenic of rats in natural recovery and Ginkgo biloba treatment groups were lower than that of arsenic exposure group (P ＜ 0.05).The content of urine arsenic of rats in natural recovery group was not different from that of Ginkgo biloba treatment group (P ＞ 0.05).The positive rates of CD3+,CD4+ [(31.31 ± 7.73)％,(25.94 ± 12.49)％] and CD4+/CD8+ ratio (0.91 ± 0.50) of arsenic exposure group in peripheral blood were lower than those of the control group [(60.83 ± 17.64)％,(42.11 ± 12.92)％,1.80 ± 0.88,all P ＜ 0.05],but complement C4 level [(81.18 ± 13.23) mg/L] in serum was significantly higher than that of the control group [(64.23 ± 6.97) mg/L,P＜ 0.01); the positive rates of CD3+,CD4+ [(32.35 ± 19.84)％,(28.00 ± 16.10)％],the ratio of CD4+/CD8+ (0.98 ± 0.29) of natural recovery group in peripheral blood and IgG,IgA,IgM,C3,C4 levels [(897.23 ± 23.55),(1 032.72 ± 0.35),(443.65 ± 1.02),(557.33 ± 39.86),(76.92 ± 17.99) mg/L] of natural recovery group in serum were not significantly different from those of arsenic exposure group [(31.31 ± 7.73)％,(25.94 ± 12.49)％,0.91 ± 0.50,(917.02 ± 15.96),(1032.96 ± 1.32),(444.43 ± 1.82),(560.15 ± 39.57),(81.18 ± 13.23)mg/L,all P ＞ 0.05); the positive rates of CD3+,CD4+ cells [(54.00 ± 19.31)％,(39.93 ± 8.63)％] of Ginkgo biloba treatment group were significantly higher than those of the arsenic exposure group [(31.31 ± 7.73)％,(25.94 ± 12.49)％,all P ＜ 0.05]; complement C4 level [(66.99 ± 5.66)mg/L] in serum was lower than that of the arsenism group [(64.23 ± 6.97) mg/L,P ＜ 0.01]; the positive rates of CD8+ in peripheral blood and IgG,IgA,IgM,C3 levels in serum of arsenic exposure group,natural recovery group,ginkgo biloba treatment group [(31.38 ± 11.40)％,(917.02 ± 15.96),(1 032.96 ± 1.32),(444.43 ± 1.82),(560.15 ± 39.57) mg/L; (27.90 ± 13.22)％,(897.23 ± 23.55),(1 032.72 ± 0.35),(443.65 ± 1.02),(557.33 ± 39.86)mg/L; (28.21 ± 8.42)％,(905.83 ± 24.16),(1 032.10 ± 0.80),(442.65 ± 1.32),(554.41 ± 41.21)mg/L] were not significantly different from those of the control group [(27.22 ± 13.00)％,(903.02 ± 14.69),(1 032.04 ± 0.64),(443.41 ± 0.93),(536.61 ± 32.92) mg/L,all P ＞ 0.05].Conclusions Arsenic can cause immune dysfunction in vivo in rat; even after ceased exposure to arsenic,the natural recovery is not good; Ginkgo biloba can enhance the immune function in arsenic poisoning rats.
