CAJ | 학술논문

目的寻找尿砷测定中合适的尿样前处理方法以及仪器最佳分析条件.方法收集健康成人晨尿20份,采用湿法消解、恒温消解和微波消解3种前处理方法对尿样进行处理,一定浓度硝酸溶液作为载流,硫脲+抗坏血酸溶液作为预还原剂,并以氢氧化钾和硼氢化钾为还原剂,用原子荧光分光光度计测定尿中砷含量.结果湿法消解、恒温消解和微波消解3种方法进行处理后的尿样,分别采用5.00％硝酸作为载流、5.00％的硫脲+5.00％抗坏血酸溶液作为预还原剂、0.50％氢氧化钾和1.00％硼氢化钾作为还原剂的分析条件下,进行连续3d平行测定,砷回收率分别为77.51％～ 78.18％、90.64 ～ 96.15％、90.96％～ 98.23％,相对标准偏差分别为2.29％、2.17％、1.88％.结论在5.00％硝酸作为载流、5.00％的硫脲+5.00％抗坏血酸溶液作为预还原剂、0.50％氢氧化钾和1.00％硼氢化钾作为还原剂的分析条件下,微波消解这种前处理方法所获得的回收率较好,是最为理想的前处理方法.
목적 심조뇨신측정중합괄적뇨양전처리방법이급의기최가분석조건.방법 수집건강성인신뇨20빈,채용습법소해、항온소해화미파소해3충전처리방법대뇨양진행처리,일정농도초산용액작위재류,류뇨+항배혈산용액작위예환원제,병이경양화갑화붕경화갑위환원제,용원자형광분광광도계측정뇨중신함량.결과 습법소해、항온소해화미파소해3충방법진행처리후적뇨양,분별채용5.00％초산작위재류、5.00％적류뇨+5.00％항배혈산용액작위예환원제、0.50％경양화갑화1.00％붕경화갑작위환원제적분석조건하,진행련속3d평행측정,신회수솔분별위77.51％～ 78.18％、90.64 ～ 96.15％、90.96％～ 98.23％,상대표준편차분별위2.29％、2.17％、1.88％.결론 재5.00％초산작위재류、5.00％적류뇨+5.00％항배혈산용액작위예환원제、0.50％경양화갑화1.00％붕경화갑작위환원제적분석조건하,미파소해저충전처리방법소획득적회수솔교호,시최위이상적전처리방법.
Objective To determine a uitable sample pretreatment method in determination of arsenic in urine and abest analysis condition of the instrument.Methods Twenty copies of morning urine samples of health adults were collected; three pretreatment methods of wet digestion,constant temperature digestion and microwave digestion were used to deal with eachurine sample.A certain concentration of nitric acid solution as the carrier,thiourea + L-ascorbic acid solution as a pre-reducing agent,and potassium hydroxide and potassium borohydride as reducing agent,arsenic content in urine was determined by atomic fluorescence spectrometry.Results Using 5.00％ nitric acid solution as the carrier,5.00％ thiourea + 5.00％ L-ascorbicacid solution as apre-reducing agent,0.50％ potassium hydroxide and 1.00％ potassium borohydride as reducing agent,urine samples treated with wet digestion,constant temperature digestion and microwave digestion were parallel determined of urinary arsenic for three consecutive days.Recoveries were 77.15％-78.18％,90.64-96.15％ and 90.96％-98.23％ with relative standard deviations were 2.29％,2.17％ and 1.88％,respectively.Conclusions Under the analysis conditions of 5.00％ nitric acid solution as the carrier,5.00％ thiourea + 5.00％ L-ascorbic acid solution as apre-reducing agent,0.50％ potassium hydroxide and 1.00％ potassium borohydride as reducing agent,the recovery of microwave digestion is better than that of the wet digestion and the constant temperature digestion.Microwave digestion is aideal pretreatment method.