山楂承气汤对高脂血症性胰腺炎大鼠肠道屏障功能障碍的治疗作用
산사승기탕대고지혈증성이선염대서장도병장공능장애적치료작용
Role of junctional adhesion molecule C in the pathogenesis of hyperlipidemic acute pancreatitis and the effect of Shanzhachengqi Decoction on its expression
  • 万方数据
    中华胰腺病杂志 중화이선병잡지
    CHINESE JOURNAL OF PANCREATOLOGY
    2014年 6期 396-401 ,共6页

    陈亚峰%田继云%谢金昆%王康%李红昌%孔娉婷%樊信娟%涂佳敏%潘丽芸

    진아봉%전계운%사금곤%왕강%리홍창%공빙정%번신연%도가민%반려예

    胰腺炎,急性坏死性%高脂血症%毛细血管通透性%连接黏附分子C%山楂承气汤 胰腺炎,急性壞死性%高脂血癥%毛細血管通透性%連接黏附分子C%山楂承氣湯
    이선염,급성배사성%고지혈증%모세혈관통투성%련접점부분자C%산사승기탕
    Pancreatitis,aucte necrotizing%Hyperlipidemias%Gapillary permeability%Junctional adhesion molecule C%Shanzhadachengqi decoction
    目的 观察山楂承气汤对高脂血症性急性坏死性胰腺炎(HANP)大鼠肠道毛细血管内皮屏障功能障碍的治疗效果,探讨其可能的机制.方法 雄性Sprague-Dawley大鼠120只,按数字表法随机分为高脂血症组(HL)、HANP组、辛伐他汀组和山楂承气汤组.采用高脂饲料喂养4周加静脉注入Triton WR1339的方法制备高胆固醇、高三酰甘油血症模型,后3组再采用腹腔内注射雨蛙素和脂多糖的方法制备急性坏死性胰腺炎(ANP)形成HANP模型.辛伐他汀组于HANP制模前2h及制模后2、4、12h分别用辛伐他汀药液灌胃1次,每次2.6 mg/kg体质量.山楂承气汤组同时间点灌胃1次,每次2 ml汤剂.制模后6、12、24 h分别处死大鼠,处死前1h经阴茎静脉注入2％伊文思蓝染料.检测各组大鼠血清淀粉酶、三酰甘油(TG)、游离脂肪酸(FFA)、连接黏附分子C(JAM-C)含量;观察肠壁组织形态学及超微结构改变;检测肠壁组织毛细血管通透性及JAM-C mRNA表达量.结果 与HL组比较,HANP组大鼠血清JAM-C、TG、FFA含量差异无统计学意义,但血清淀粉酶活性显著升高[(4963±531)U/L比(885±28) U/L,P＜0.01],肠壁的病理组织学及超微结构损伤显著加重,肠壁组织毛细血管通透性及JAN-C mRNA表达量(4.46±0.16比2.08±0.35)均显著升高,差异均有统计学意义(P＜0.01).与HANP组比较,山楂承气汤组淀粉酶活性[(991±61) U/L比(4963±531) U/L]、TG含量[(20.68±2.68) mmol/L比(95.73±2.19) mmol/L]、FFA含量[(1238±37) μmol/L比(3879 ±64) μmol/L]、JAM-C含量[(166.44±10.26) ng/L比(396.99±12.15) ng/L]均显著下降(P值均＜0.01),肠壁的病理组织学及超微结构损伤显著减轻,肠壁组织毛细血管通透性及JAM-C mRNA表达量(1.38± 0.10比4.46±0.16)均显著下降,差异均有统计学意义(P值均＜0.01).辛伐他汀组各指标的下降程度均低于山楂承气汤组.结论 山楂承气汤可以改善HANP大鼠的病情,减轻肠壁组织毛细血管渗漏,其机制可能与下调肠壁组织JAM-C的表达有关.
    목적 관찰산사승기탕대고지혈증성급성배사성이선염(HANP)대서장도모세혈관내피병장공능장애적치료효과,탐토기가능적궤제.방법 웅성Sprague-Dawley대서120지,안수자표법수궤분위고지혈증조(HL)、HANP조、신벌타정조화산사승기탕조.채용고지사료위양4주가정맥주입Triton WR1339적방법제비고담고순、고삼선감유혈증모형,후3조재채용복강내주사우와소화지다당적방법제비급성배사성이선염(ANP)형성HANP모형.신벌타정조우HANP제모전2h급제모후2、4、12h분별용신벌타정약액관위1차,매차2.6 mg/kg체질량.산사승기탕조동시간점관위1차,매차2 ml탕제.제모후6、12、24 h분별처사대서,처사전1h경음경정맥주입2％이문사람염료.검측각조대서혈청정분매、삼선감유(TG)、유리지방산(FFA)、련접점부분자C(JAM-C)함량;관찰장벽조직형태학급초미결구개변;검측장벽조직모세혈관통투성급JAM-C mRNA표체량.결과 여HL조비교,HANP조대서혈청JAM-C、TG、FFA함량차이무통계학의의,단혈청정분매활성현저승고[(4963±531)U/L비(885±28) U/L,P＜0.01],장벽적병리조직학급초미결구손상현저가중,장벽조직모세혈관통투성급JAN-C mRNA표체량(4.46±0.16비2.08±0.35)균현저승고,차이균유통계학의의(P＜0.01).여HANP조비교,산사승기탕조정분매활성[(991±61) U/L비(4963±531) U/L]、TG함량[(20.68±2.68) mmol/L비(95.73±2.19) mmol/L]、FFA함량[(1238±37) μmol/L비(3879 ±64) μmol/L]、JAM-C함량[(166.44±10.26) ng/L비(396.99±12.15) ng/L]균현저하강(P치균＜0.01),장벽적병리조직학급초미결구손상현저감경,장벽조직모세혈관통투성급JAM-C mRNA표체량(1.38± 0.10비4.46±0.16)균현저하강,차이균유통계학의의(P치균＜0.01).신벌타정조각지표적하강정도균저우산사승기탕조.결론 산사승기탕가이개선HANP대서적병정,감경장벽조직모세혈관삼루,기궤제가능여하조장벽조직JAM-C적표체유관.
    Objective To investigate the therapeutic efficacy of Shanzhadachengqi Decoction on the capillary endothelial cell barrier dysfunction of the intestinal tract in hyperlipidemic acute necrotizing pancreatitis (HANP) rats,and to explore the possible molecular mechanism.Methods Male Sprague-Dawley rats (n =120) were randomly divided into hyperlipidemic (HL) group,HANP group,simvastatin group and Shanzhadachengqi Decoction(SD) group.The hyperlipidemic model was established by 4 weeks fat-rich food and Triton WR1339 intravenous injection.The rats in the latter 3 groups underwent intraperitoneal injection of cerulein and lipopolysaccharid to induce the acute necrotizing pancreatitis (ANP) (HANP model).The rats in simvastatin group received simvastatin gastric lavage (2.6 mg/kg) 2 h before HANP model induction,2,4,12 h after HANP model induction,while the rats in SD group received Shanzhadachengqi Decoction gastric lavage (2 ml) at the same time points,then the rats were sacrificed at 6,12,and 24 h after model induction.One hour before the rats were sacrificed,they were injected with 2％ Evens Blue.Levels of serum amylases,triacylglycerol (TG),free fatty acid (FFA) and junctional adhesion molecule C (JAM-C) were examined; the pathological changes and ultramicrostructure in intestine tissues were observed; expression of JAM-C mRNA and capillary permeability in intestine tissues were determined.Results The serum levels of JAM-C,TG,FFA in HANP group was not significantly different from those in the HL group,but the serum level of amylase was significantly increased [(4 963 ± 531) vs (885 ± 28) U/L,P =0.003].The pathology change and the ultramicrostructure showed histopathological changes in intestine tissues in HANP group were more severe,and the intestine tissular capillary permeability [(201.83 ± 8.50) vs (54.23 ± 5.79) μg/ml,P =0.000],the expression of JAM-C mRNA [(4.46 ±0.16) vs (2.08 ±0.35),P=0.002)] was significantly higher than those in the HL group,and the difference between the two groups was statistically significant (P ＜ 0.05).Compared with HANP group,the serum level of amylases [(991 ±61) vs (4 963 ±531)U/L,P=0.003],TG [(20.68 ± 2.68) vs (95.73 ± 2.190 mmol/L,P =0.000],FFA [(1 238 ± 37) vs (3 879 ± 64) μmol/ L,P=0.000],JAM-C [(166.44± 10.26) vs (396.99± 12.15) ng/L,P=0.000] in SD group were significantly decreased.Pathology change and the ultramicrostructure showed histopathological changes in intestine tissues were attenuated.The intestine tissular capillary permeability [(39.90 ± 3.62) vs (201.83 ± 8.50) μg/ml,P =0.000],the expression of JAM-C mRNA [(1.38 ± 0.10) vs (4.46 ± 0.16),P =0.003] were significantly decreased,and the difference between the two groups was statistically significant (P ＜ 0.05).The decreased degree of corresponding values in simvastatin group wsa lower than those in SD group.Conclusions Shanzhadachengqi Decoction can improve the course of HANP rat; and relieve the intestine tissuior capillary leakage,which may be related with the down-regulation of JAM-C expression in intestine tissues.
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