CAJ | 학술논문

肝脏替代治疗是目前终末期肝病最有效的治疗措施,而去细胞化肝脏生物支架拓宽了肝脏替代治疗的研究领域.目前普遍的支架制备过程是在一定的物理条件下,经灌注设备将化学试剂(去垢剂、消化酶等)注入肝脏自身的脉管结构中,达到去除细胞成分,保留支架内ECM和超微脉管结构的目的.再将种子细胞注入到去细胞化肝脏生物支架,获得再细胞化肝脏,模拟机体内肝脏生物环境进行体外或体内培养,观察种子细胞附着情况,检测肝脏代谢功能,评估肝脏替代效果.目前,种子细胞的选取、再细胞化流程、再细胞化肝脏移植等问题仍处于探索阶段.笔者围绕去细胞化肝脏生物支架的制备与评价、检测和应用作一综述,为进一步应用于实验研究和临床实践提供参考.
간장체대치료시목전종말기간병최유효적치료조시,이거세포화간장생물지가탁관료간장체대치료적연구영역.목전보편적지가제비과정시재일정적물리조건하,경관주설비장화학시제(거구제、소화매등)주입간장자신적맥관결구중,체도거제세포성분,보류지가내ECM화초미맥관결구적목적.재장충자세포주입도거세포화간장생물지가,획득재세포화간장,모의궤체내간장생물배경진행체외혹체내배양,관찰충자세포부착정황,검측간장대사공능,평고간장체대효과.목전,충자세포적선취、재세포화류정、재세포화간장이식등문제잉처우탐색계단.필자위요거세포화간장생물지가적제비여평개、검측화응용작일종술,위진일보응용우실험연구화림상실천제공삼고.
Replacement therapy is the most effective method for the treatment of end-stage liver disease,and decellularized liver bioscaffold broadens the research field of the replacement therapy.The present liver bioscaffold preparation is to perfuse chemical reagents (detergents,enzymes,et al) into the vascular structure of the liver under certain physical conditions,so as to remove cellular components and retain extracellular matrix and microvascular structure.Cells were reseeded into the decellularized liver scaffold to obtain the recellularized liver,which can be cultured and evaluated in vitro or in vivo by observing the adhesion of seeded cells,detecting the synthesis and secretion of the recellularized liver.Currently,the selection of seed cells,recellularization protocol and recellularized liver transplantation are still under exploration.In this review,the preparation,evaluation,detection and application of the decellularized liver bioscaffold are introduced for the further experimental study and clinical research.