CAJ | 학술논문

目的定量分析Cdc42对A549细胞迁移能力的独立调节作用,并验证一种自动测量和计算体外细胞迁移距离的新方法的有效性.方法在人肺腺癌A549细胞中过表达Cdc42,然后进行划痕实验并在不同时间点采集图像.应用多种测量方法对图像进行定量分析,统计分析各方法针对细胞迁移距离测量的精确度和可重复性.结果 Cdc42过表达组A549细胞的迁移速度大于对照组(P＜0.05).与人工测量相比,新方法具有高效、准确及重复性好等优势.与现有计算机软件(Image Pro Plus 6.0)测量方法相比,新方法在测量区域识别、去除噪声等方面具有显著的优越性.结论 Cdc42过表达后显著提高了A549细胞的体外迁移能力.新方法可实现对体外细胞迁移的自动定量分析.
목적 정량분석Cdc42대A549세포천이능력적독립조절작용,병험증일충자동측량화계산체외세포천이거리적신방법적유효성.방법 재인폐선암A549세포중과표체Cdc42,연후진행화흔실험병재불동시간점채집도상.응용다충측량방법대도상진행정량분석,통계분석각방법침대세포천이거리측량적정학도화가중복성.결과 Cdc42과표체조A549세포적천이속도대우대조조(P＜0.05).여인공측량상비,신방법구유고효、준학급중복성호등우세.여현유계산궤연건(Image Pro Plus 6.0)측량방법상비,신방법재측량구역식별、거제조성등방면구유현저적우월성.결론 Cdc42과표체후현저제고료A549세포적체외천이능력.신방법가실현대체외세포천이적자동정량분석.
Objective To explore whether Cdc42 is an independent influence factor in regulating the migration of A549 cells by using quantitative measurement method,and to verify the effectiveness of an automatic measurement and calculation method for in vitro cell migration assay.Methods Cdc42 was overexpressed in human lung adenocarcinoma A549 cell line,and then in vitro scratch assay was applied to evaluate the migration of the cells.Different methods were used to measure the images acquired at different time points for quantitative analysis.Accuracy and repeatability of different measurement methods were analyzed.Results The results showed that overexpression of Cdc42 alone significantly advanced the migration of A549 cells (P＜0.05).The new method is efficient,accurate and reproducible as compared to manual measurement,and has significant advantages in target recognition and noise removal as compared to the existing measurement method in Image Pro Plus 6.0.Conclusions Over expression of Cdc42 significantly increased A549 cell migration ability in vitro.The new method can realize the automatic quantitative analysis of cell migration in vitro.