国际生物医学工程杂志
國際生物醫學工程雜誌
국제생물의학공정잡지
INTERNATIONAL JOURNAL OF BIOMEDICAL ENGINEERING
2014年
6期
328-331,后插1
,共5页
苦豆子生物碱%肺癌%抗肿瘤%作用机制
苦豆子生物堿%肺癌%抗腫瘤%作用機製
고두자생물감%폐암%항종류%작용궤제
Alkaloid monoers%Lung cancer cells%Anti-tumor activity%Action mechanism
目的 对4种苦豆子生物碱的体外抗肺癌活性及机制进行研究,并评价其对正常细胞的毒性,以期筛选出具有高效低毒抗肿瘤作用的药物.方法 用噻唑蓝(MTT)法测定4种苦豆子生物碱对人肺癌细胞和仓鼠肺细胞增殖活性的影响;用激光共聚焦显微镜观察Hochest33342染色后肺癌细胞凋亡的形态学变化;用流式细胞仪检测异硫氰酸荧光素标记的膜联蛋白Ⅴ/碘化丙啶(Annexin Ⅴ-FITC/PI)双染后肺癌细胞的凋亡率.结果 4种苦豆子生物碱对肺癌细胞的生长具有不同程度的抑制作用,呈剂量依赖关系,以氧化槐定碱的抗肺癌活性最强,且对正常肺细胞的毒性较小;Hochest33342染色和流式细胞术显示氧化槐定碱可以明显诱导人非小细胞肺癌细胞A549发生凋亡和坏死.结论 4种苦豆子生物碱均具有一定的抗肺癌作用,以氧化槐定碱的活性最强,具有高效低毒的特点,适合作为抗肿瘤候选药物进一步开发.
目的 對4種苦豆子生物堿的體外抗肺癌活性及機製進行研究,併評價其對正常細胞的毒性,以期篩選齣具有高效低毒抗腫瘤作用的藥物.方法 用噻唑藍(MTT)法測定4種苦豆子生物堿對人肺癌細胞和倉鼠肺細胞增殖活性的影響;用激光共聚焦顯微鏡觀察Hochest33342染色後肺癌細胞凋亡的形態學變化;用流式細胞儀檢測異硫氰痠熒光素標記的膜聯蛋白Ⅴ/碘化丙啶(Annexin Ⅴ-FITC/PI)雙染後肺癌細胞的凋亡率.結果 4種苦豆子生物堿對肺癌細胞的生長具有不同程度的抑製作用,呈劑量依賴關繫,以氧化槐定堿的抗肺癌活性最彊,且對正常肺細胞的毒性較小;Hochest33342染色和流式細胞術顯示氧化槐定堿可以明顯誘導人非小細胞肺癌細胞A549髮生凋亡和壞死.結論 4種苦豆子生物堿均具有一定的抗肺癌作用,以氧化槐定堿的活性最彊,具有高效低毒的特點,適閤作為抗腫瘤候選藥物進一步開髮.
목적 대4충고두자생물감적체외항폐암활성급궤제진행연구,병평개기대정상세포적독성,이기사선출구유고효저독항종류작용적약물.방법 용새서람(MTT)법측정4충고두자생물감대인폐암세포화창서폐세포증식활성적영향;용격광공취초현미경관찰Hochest33342염색후폐암세포조망적형태학변화;용류식세포의검측이류청산형광소표기적막련단백Ⅴ/전화병정(Annexin Ⅴ-FITC/PI)쌍염후폐암세포적조망솔.결과 4충고두자생물감대폐암세포적생장구유불동정도적억제작용,정제량의뢰관계,이양화괴정감적항폐암활성최강,차대정상폐세포적독성교소;Hochest33342염색화류식세포술현시양화괴정감가이명현유도인비소세포폐암세포A549발생조망화배사.결론 4충고두자생물감균구유일정적항폐암작용,이양화괴정감적활성최강,구유고효저독적특점,괄합작위항종류후선약물진일보개발.
Objective To investigate in vitro anti-tumor activity and the related mechanism of four alkaloid monomers from Sophora alopecuroides,and to evaluate their toxicity on normal cells,thereby to screen the drugs with high efficiency and low toxicity anti-tumor effects.Methods Methyl thiazolyl tetrazolium (MTT) assay was employed to determine the effects of four alkaloid monomers on the proliferation activity of human lung cancer cells and hamster lung cells.Morphology of apoptotic lung cancer cell nucleus was observed by laser scanning confocal microscopy with Hochest33342 staining.Cell apoptosis and necrosis were measured by Annexin Ⅴ-FITC/PI double staining.Results The four alkaloid monomers showed different degree of proliferation inhibition on lung cancer cells with dose dependent manner.Oxysophoridine had the strongest inhibition effect on lung cancer cells and the minimun toxicity on normal lung cells.Hochest33342 staining and flow cytometry analysis demonstrated that oxysophoridine could induce the apoptosis and necrosis of A549 cells.Conclusions All four alkaloid monomers have certain anti-tumor effect on lung cancer cells.Oxysophoridine is the most active one with high efficiency and low toxicity characteristics,which is suitable for use as anti-tumor drug candidate for further development.
