CAJ | 학술논문

目的探讨人初乳和成熟乳中免疫相关microRNA(miR)的表达水平及其与母婴健康的关系.方法选择产科分娩的母婴为研究对象,按喂养方式分为人乳喂养组(86例)和配方乳喂养组(48例),并随访至婴儿3月龄,记录母婴的营养状况及疾病状况.实时荧光定量聚合酶链反应(Real-time PCR)检测人乳中miR-146b、miR-155及miR-30b表达水平,并分析其与母婴健康的关系.结果 1.于2012年11月至2013年2月收集人初乳86份及配对的成熟乳34份.人初乳中miR-146b(5.950±0.823)及miR-155(3.899±0.920)表达水平高于成熟乳(4.840±0.805、2.128±0.969),差异有统计学意义(=7.176、10.215,P均＜0.01),而成熟乳中miR-30b表达(4.929±0.566)高于初乳(4.057±0.604),差异有统计学意义(t=-8.626,P＜0.01).2.初乳中miR-30b的水平与母亲孕前体质量指数呈负相关(r=-0.298,P＜0.01).3.自然分娩组母亲初乳中miR-30b、miR-146b的水平高于剖宫产组(t=3.108、2.356,P均＜0.05).4.分娩女婴的母亲初乳中miR-146b、miR-155水平高于男婴(t=-2.204、-2.985,P均＜0.05).5.成熟乳中miR-146b表达水平与随访时婴儿体质量的Z评分(r=-0.425,P＜0.05)、身长的Z评分(r=-0.569,P＜0.01)呈负相关.6.随访期间,人乳喂养婴儿湿疹发生率(8.82％,3/34例)明显低于配方牛乳喂养组(29.17％,14/48例)(x2=5.012,P=0.025).结论免疫相关的miR-146b、miR-155及miR-30b在人乳中含量丰富,并与母亲分娩方式、孕前体质量指数和婴儿性别相关.人乳中持续存在的免疫相关microRNA可能参与调节婴儿体格生长和免疫功能发育.
목적 탐토인초유화성숙유중면역상관microRNA(miR)적표체수평급기여모영건강적관계.방법 선택산과분면적모영위연구대상,안위양방식분위인유위양조(86례)화배방유위양조(48례),병수방지영인3월령,기록모영적영양상황급질병상황.실시형광정량취합매련반응(Real-time PCR)검측인유중miR-146b、miR-155급miR-30b표체수평,병분석기여모영건강적관계.결과 1.우2012년11월지2013년2월수집인초유86빈급배대적성숙유34빈.인초유중miR-146b(5.950±0.823)급miR-155(3.899±0.920)표체수평고우성숙유(4.840±0.805、2.128±0.969),차이유통계학의의(=7.176、10.215,P균＜0.01),이성숙유중miR-30b표체(4.929±0.566)고우초유(4.057±0.604),차이유통계학의의(t=-8.626,P＜0.01).2.초유중miR-30b적수평여모친잉전체질량지수정부상관(r=-0.298,P＜0.01).3.자연분면조모친초유중miR-30b、miR-146b적수평고우부궁산조(t=3.108、2.356,P균＜0.05).4.분면녀영적모친초유중miR-146b、miR-155수평고우남영(t=-2.204、-2.985,P균＜0.05).5.성숙유중miR-146b표체수평여수방시영인체질량적Z평분(r=-0.425,P＜0.05)、신장적Z평분(r=-0.569,P＜0.01)정부상관.6.수방기간,인유위양영인습진발생솔(8.82％,3/34례)명현저우배방우유위양조(29.17％,14/48례)(x2=5.012,P=0.025).결론 면역상관적miR-146b、miR-155급miR-30b재인유중함량봉부,병여모친분면방식、잉전체질량지수화영인성별상관.인유중지속존재적면역상관microRNA가능삼여조절영인체격생장화면역공능발육.
Objective To explore the expression levels of immune-related microRNA-146b (miR-146b),microRNA-155 (miR-155) and microRNA-30b(miR-30b) in human breast milk and its relationship with maternal and infant's health.Methods One hundred and thirty-four mothers and their infants from obstetrical department were recruited in the study after delivery.The subjects were divided into 2 groups,breast feeding group(n =86) and formula-feeding group(n =48),and were followed up 3 months after delivery.Breast milk samples were collected at 2-5 days after delivery(colostrum) and 3 months after delivery(mature milk).The expression levels of microRNAs in milk samples were detected by real-time PCR.The relationship between levels of microRNAs and maternal and infant-related factors was analyzed.Results 1.MiR-146b,miR-155 and miR-30b expressions were abundant both in human colostrums (5.950 ± 0.823,3.899 ± 0.920,4.057 ± 0.604) and mature milk (4.840 ± 0.805,2.128 ± 0.969,4.929 ± 0.566).The levels of miR-146b and miR-155 were higher in colostrum than that of mature milk (t =7.716,10.215,all P ＜ 0.01),while the level of miR-30b was higher in mature milk than that of colostrums(t =-8.626,P ＜ 0.0l).2.Additionally,the level of miR-30b was negatively correlated with maternal pre-pregnancy body mass index (r =-0.298,P ＜ 0.01).3.The levels of miR-146b and miR-30b were higher in mothers giving birth by vaginal delivery than those who underwent cesarean section(t =2.356,3.108,all P ＜0.05).4.The levels of miR-146b and miR-155 were higher in colostrum-fed girls than boys (t =-2.204,-2.985,all P ＜ 0.05).5.The level of miR-146b in mature milk was negatively correlated with 3-month-old infant' s Z score of body weight (r =-0.425,P ＜ 0.05) and body length (r =-0.569,P ＜ 0.01).6.During follow-up,the incidence of baby eczema in breast feeding group (8.82％,3/34 cases) was lower than that in formula milk feeding group(29.17％,14/48 cases) (x2 =5.012,P =0.025).Conclusions The levels of immunocompetent microRNAs in human milk are influenced by the lactation period,maternal prepregnancy body mass index,mode of delivery and infant sex.The immune-related microRNAs in human milk could be involved in the regulation of infant's immunity and growth.