CAJ | 학술논문

目的了解昆明地区新生儿葡萄糖-6-磷酸脱氢酶(G6PD)水平,以辅助筛查新生儿G6PD缺乏症.方法采集昆明地区29 527例新生儿足跟血滤纸片干血斑标本,采用荧光分析法检测G6PD水平.采用SPSS 13.0统计软件进行数据分析.结果 29 527例滤纸片干血斑标本的G6PD水平为0.10 ～ 15.95 U/g Hb,平均为(6.18±1.04) U/g Hb.男、女新生儿G6PD水平分别为(6.16±1.10) U/g Hb和(6.21±1.00) U/gHb,男女G6PD水平比较差异有统计学意义(t=-4.095,P＜0.05);按不同采血年龄分为3组,分别为＜3d采血组、3～7d采血组和8～28 d采血组,其G6PD水平分别为(6.13±1.05) U/gHb、(6.20±1.03) U/gHb和(6.13±1.07) U/g Hb.＜3d采血组和8～28 d采血组间G6PD水平比较差异无统计学意义(t=0.029,P＞0.05),这2组G6PD水平与3～7d组比较差异均有统计学意义(P均＜0.05),且G6PD水平均值低于3～7d组;按不同检测水平分为3组,分别为＜ 2.20 U/g Hb组、2.20 ～2.60 U/g Hb组和＞2.60 U/g Hb组.＜2.20U/g Hb组中男、女新生儿构成比与另外2组比较差异均有统计学意义(P均＜0.05).结论昆明地区G6PD缺乏症符合X伴性遗传方式,男性检出率高于女性;新生儿期任意时间采血对G6PD阳性检出率无影响;昆明地区G6PD缺乏症流行病学有其独特的特点,检出率有下降趋势.
목적 료해곤명지구신생인포도당-6-린산탈경매(G6PD)수평,이보조사사신생인G6PD결핍증.방법 채집곤명지구29 527례신생인족근혈려지편간혈반표본,채용형광분석법검측G6PD수평.채용SPSS 13.0통계연건진행수거분석.결과 29 527례려지편간혈반표본적G6PD수평위0.10 ～ 15.95 U/g Hb,평균위(6.18±1.04) U/g Hb.남、녀신생인G6PD수평분별위(6.16±1.10) U/g Hb화(6.21±1.00) U/gHb,남녀G6PD수평비교차이유통계학의의(t=-4.095,P＜0.05);안불동채혈년령분위3조,분별위＜3d채혈조、3～7d채혈조화8～28 d채혈조,기G6PD수평분별위(6.13±1.05) U/gHb、(6.20±1.03) U/gHb화(6.13±1.07) U/g Hb.＜3d채혈조화8～28 d채혈조간G6PD수평비교차이무통계학의의(t=0.029,P＞0.05),저2조G6PD수평여3～7d조비교차이균유통계학의의(P균＜0.05),차G6PD수평균치저우3～7d조;안불동검측수평분위3조,분별위＜ 2.20 U/g Hb조、2.20 ～2.60 U/g Hb조화＞2.60 U/g Hb조.＜2.20U/g Hb조중남、녀신생인구성비여령외2조비교차이균유통계학의의(P균＜0.05).결론 곤명지구G6PD결핍증부합X반성유전방식,남성검출솔고우녀성;신생인기임의시간채혈대G6PD양성검출솔무영향;곤명지구G6PD결핍증류행병학유기독특적특점,검출솔유하강추세.
Objective To investigate the concentration of glucose-6-phosphate dehydrogenase (G6PD) concentration in newborns in Kunming areas for better assisting screen of G6PD deficiency among the live newborns.Methods A total of 29 527 bloodspot filter paper specimens were collected from newborns in Kunming.The fluorescence analysis was used to detect G6PD concentration.All statistical analyses were conducted by using SPSS 13.0 software.Results The G6PD concentration of 29 527 bloodspot filter paper specimens was 0.10-15.95 U/g Hb,the average value was(6.18 ± 1.04) U/g Hb.The average G6PD concentration of male and female newborns were(6.16 ± 1.10) U/g Hb and(6.21 ± 1.00) U/g Hb,respectively.The average G6PD enzyme concentration of female newborns was significantly higher than that of the male newborns(t =-4.095,P ＜ 0.05).The newborns were divided into three groups according to ages,the group of less than 3 days group,3-7 days group and 8-28 days group.The average G6PD enzyme concentration of 3 groups were(6.13 ± 1.05) U/g Hb,(6.20 ± 1.03) U/g Hb and (6.13 ± 1.07) U/g Hb,respectively.There was no statistical difference between the less than 3 days group and 8-28 days group in the average G6PD enzyme concentration (t =0.029,P ＞ 0.05),but the average G6PD enzyme concentration of these 2 groups were significantly lower than that of 3-7 days group(all P ＜ 0.05).According to concentrations,they were divied into ＜ 2.20 U/g Hb group,2.20-2.60 U/g Hb group and ＞ 2.60 U/g Hb group.The gender ratio in ＜ 2.20 U/g Hb group was statistically different between 2.20-2.60 U/g Hb group and ＞ 2.60 U/g Hb group(all P ＜ 0.05).Conclusions X-linked pattern of inheritance of G6PD deficiency is also observed in Kunming.The detection rate in male newborns is higher than that of female newborns.The specimen acquisition time in the neonatal period has no influence on the detection rate of the G6PD deficiency.G6PD deficiency epidemiology has its own unique features in Kunming.It shows a declining trend after years of diagnosis and intervention.