CAJ | 학술논문

目的制备3种用于蛋白类药物载体的可降解膜材料,并比较其降解性能和释药性能.方法制备了3种不同的可降解缓控释放膜,即明胶膜、壳寡糖膜和交联壳寡糖膜.利用考马斯亮蓝测定蛋白释放量,确定膜的降解率和溶胀率.同时,采用噻唑蓝(MTT)法考察膜的生物相容性.结果交联壳寡糖膜的释放时间(168h)大于壳寡糖膜,且不同介质中的释放时间不同,降解率和溶胀率分别为60％(360h)和110.45％,而壳寡糖膜的降解率和溶胀率分别为80％(360h)和113.03％.MTT结果表明,交联壳寡糖膜具有较好的生物相容性.结论通过对比分析,最终确定交联壳寡糖膜为最佳蛋白类药物载体.
목적 제비3충용우단백류약물재체적가강해막재료,병비교기강해성능화석약성능.방법 제비료3충불동적가강해완공석방막,즉명효막、각과당막화교련각과당막.이용고마사량람측정단백석방량,학정막적강해솔화용창솔.동시,채용새서람(MTT)법고찰막적생물상용성.결과 교련각과당막적석방시간(168h)대우각과당막,차불동개질중적석방시간불동,강해솔화용창솔분별위60％(360h)화110.45％,이각과당막적강해솔화용창솔분별위80％(360h)화113.03％.MTT결과표명,교련각과당막구유교호적생물상용성.결론 통과대비분석,최종학정교련각과당막위최가단백류약물재체.
Objective To prepare three kinds of biodegradable film materials used for protein drug carrier,and compare their degradation and drug release behavior.Methods Three different biodegradable and controlled release films,gelatin,chitosan oligosaccharides and crosslinked chitosan oligosaccharides films were prepared.Protein release behavior was determined by the Bradford.At the same time,degradation rate and swelling rate were tested,and the biocompatibility of film was investigated by MTT assay.Results The release time of crosslinked chitosan oligosaccharides film was 168 h,which was longer than that of chitosan oligosaccharides film,and different in different solution.The degradation rate and the swelling rate of crosslinked chitosan oligosaccharides was 60％ (360 h) and 110.45％,respectively,while the chitosan oligosaccharides membrane was 80％ (360 h) and 113.03％.The MTI assay revealed that the crosslinked chitosan oligosaccharides film had better biocompatibility.Conclusions By comparing different properties,the crosslinked chitosan oligosaccharides film is the best choice for protein drug carrier.