CAJ | 학술논문

目的探讨肿瘤坏死因子-α(TN F-α)在红细胞衰亡中的作用.方法将分离的小鼠红细胞经TNF-α(1 ng/ml)处理6、12、24、48、72 h或在不同质量浓度(0.1、1、10 ng/ml)处理24h后,利用流式细胞仪检测红细胞前向散射(FSC)值的变化、红细胞膜磷脂酰丝氨酸(PS)和神经酰胺的标记率.结果经TNF-α处理后的小鼠红细胞较对照组于24h后出现FSC减小((81.5±1.02)％比(87.6±0.55)％,P＜0.05),膜的PS外露水平和神经酰胺含量增加((5.5±1.07)％比(2.7±0.17)％,(2.1±0.23)％比(0.7±0.26)％),差异均有统计学意义(P＜0.01),且随着时间的推移变化趋势更加明显.结论 TNF-α可诱导红细胞体积缩小,促进红细胞膜PS外露以及神经酰胺的增加,是红细胞衰亡的触发因素.
목적 탐토종류배사인자-α(TN F-α)재홍세포쇠망중적작용.방법 장분리적소서홍세포경TNF-α(1 ng/ml)처리6、12、24、48、72 h혹재불동질량농도(0.1、1、10 ng/ml)처리24h후,이용류식세포의검측홍세포전향산사(FSC)치적변화、홍세포막린지선사안산(PS)화신경선알적표기솔.결과 경TNF-α처리후적소서홍세포교대조조우24h후출현FSC감소((81.5±1.02)％비(87.6±0.55)％,P＜0.05),막적PS외로수평화신경선알함량증가((5.5±1.07)％비(2.7±0.17)％,(2.1±0.23)％비(0.7±0.26)％),차이균유통계학의의(P＜0.01),차수착시간적추이변화추세경가명현.결론 TNF-α가유도홍세포체적축소,촉진홍세포막PS외로이급신경선알적증가,시홍세포쇠망적촉발인소.
Objective To investigate the role of tumor necrosis factor-α (TNF-α) on eryptosis.Methods Erythrocytes isolated from mice were put under the treatment of TNF-α at the dose of 1ng/ml for 6,12,24,48 and 72 h,or at different concentrations of 0.1,1 and 10 ng/ml for 24 h.The forward scatter (FSC),phosphatidylserine (PS)exposure and ceramide formation were determined by flow cytometry.Results Compared to control group,the decrease of FSC ((81.5 ± 1.02)％ vs (87.6 ± 0.55)％,P＜0.05),the increasment of membrane PS exposure level and ceramide content ((5.5±1.07)％ vs (2.7±0.17)％,(2.1±0.23)％ vs (0.7±0.26)％,P＜0.01) were observed in erythrocyte under the treatment of TNF-α for 24 h with more obvious tendency over time.Conclusions TNF-α can trigger cell shrinkage,and promote PS exposure and ceramide formation on the membrane of erythrocyte.