目的 探讨去白细胞悬浮红细胞制剂储存过程中活性氧族(ROS)含量及丙二醛(MDA)含量的变化.方法 从2013年5～6月于河北省血液中心制备的去白细胞悬浮红细胞制剂中,采用简单随机抽样方法抽取6份去白细胞悬浮红细胞制剂标本作为研究对象.于储存后第0,7,14,28,42天,采用荧光探针2',7'-二氧荧光黄双乙酸盐(DCFH-DA)孵育的方法观察去白细胞悬浮红细胞荧光强度,并对去白细胞悬浮红细胞制剂内ROS含量与MDA含量进行检测.对储存后去白细胞悬浮红细胞制剂内ROS含量、MDA含量分别与储存时间进行相关性分析;并对储存后去白细胞悬浮红细胞制剂内ROS含量与MDA含量进行相关性分析.结果 ①随着储存时间的延长,经荧光探针DCFH-DA孵育的去白细胞悬浮红细胞的荧光强度显著增强;②储存后第0,7,14,28,42天去白细胞悬浮红细胞制剂内ROS含量呈显著增高趋势,第42天去白细胞悬浮红细胞内ROS含量显著高于第0,7,14,28天ROS含量,并且差异均有统计学意义(P＜0.01,0.05,0.05,0.05);且去白细胞悬浮红细胞制剂内ROS含量与储存时间呈正相关关系(r=0.898,P＜0.01);③储存后第0,7,14,28,42天去白细胞悬浮红细胞内MDA含量呈显著增高趋势,第42天去白细胞悬浮红细胞内MDA含量显著高于第0,7,14,28天MDA含量,并且差异均有统计学意义(P＜0.01,0.01,0.01,0.05);且去白细胞悬浮红细胞内MDA含量与储存时间呈正相关关系(r=0.930,P＜0.01);④储存后第0,7,14,28,42天,去白细胞悬浮红细胞制剂内ROS含量均分别与对应时间点MDA含量呈正相关关系(r=0.877,0.872,0.823,0.786,0.907;P＜0.05).去白细胞悬浮红细胞制剂储存后第0,7,14,28,42天,各时间点ROS含量均值与MDA含量均值亦呈正相关性关系(r=0.981,P＜0.01).结论 去白细胞悬浮红细胞制剂储存过程中ROS含量与MDA含量均显著增高,红细胞内氧化应激反应增强,其为导致去白细胞悬浮红细胞制剂储存损伤的重要原因.
目的 探討去白細胞懸浮紅細胞製劑儲存過程中活性氧族(ROS)含量及丙二醛(MDA)含量的變化.方法 從2013年5～6月于河北省血液中心製備的去白細胞懸浮紅細胞製劑中,採用簡單隨機抽樣方法抽取6份去白細胞懸浮紅細胞製劑標本作為研究對象.于儲存後第0,7,14,28,42天,採用熒光探針2',7'-二氧熒光黃雙乙痠鹽(DCFH-DA)孵育的方法觀察去白細胞懸浮紅細胞熒光彊度,併對去白細胞懸浮紅細胞製劑內ROS含量與MDA含量進行檢測.對儲存後去白細胞懸浮紅細胞製劑內ROS含量、MDA含量分彆與儲存時間進行相關性分析;併對儲存後去白細胞懸浮紅細胞製劑內ROS含量與MDA含量進行相關性分析.結果 ①隨著儲存時間的延長,經熒光探針DCFH-DA孵育的去白細胞懸浮紅細胞的熒光彊度顯著增彊;②儲存後第0,7,14,28,42天去白細胞懸浮紅細胞製劑內ROS含量呈顯著增高趨勢,第42天去白細胞懸浮紅細胞內ROS含量顯著高于第0,7,14,28天ROS含量,併且差異均有統計學意義(P＜0.01,0.05,0.05,0.05);且去白細胞懸浮紅細胞製劑內ROS含量與儲存時間呈正相關關繫(r=0.898,P＜0.01);③儲存後第0,7,14,28,42天去白細胞懸浮紅細胞內MDA含量呈顯著增高趨勢,第42天去白細胞懸浮紅細胞內MDA含量顯著高于第0,7,14,28天MDA含量,併且差異均有統計學意義(P＜0.01,0.01,0.01,0.05);且去白細胞懸浮紅細胞內MDA含量與儲存時間呈正相關關繫(r=0.930,P＜0.01);④儲存後第0,7,14,28,42天,去白細胞懸浮紅細胞製劑內ROS含量均分彆與對應時間點MDA含量呈正相關關繫(r=0.877,0.872,0.823,0.786,0.907;P＜0.05).去白細胞懸浮紅細胞製劑儲存後第0,7,14,28,42天,各時間點ROS含量均值與MDA含量均值亦呈正相關性關繫(r=0.981,P＜0.01).結論 去白細胞懸浮紅細胞製劑儲存過程中ROS含量與MDA含量均顯著增高,紅細胞內氧化應激反應增彊,其為導緻去白細胞懸浮紅細胞製劑儲存損傷的重要原因.
목적 탐토거백세포현부홍세포제제저존과정중활성양족(ROS)함량급병이철(MDA)함량적변화.방법 종2013년5～6월우하북성혈액중심제비적거백세포현부홍세포제제중,채용간단수궤추양방법추취6빈거백세포현부홍세포제제표본작위연구대상.우저존후제0,7,14,28,42천,채용형광탐침2',7'-이양형광황쌍을산염(DCFH-DA)부육적방법관찰거백세포현부홍세포형광강도,병대거백세포현부홍세포제제내ROS함량여MDA함량진행검측.대저존후거백세포현부홍세포제제내ROS함량、MDA함량분별여저존시간진행상관성분석;병대저존후거백세포현부홍세포제제내ROS함량여MDA함량진행상관성분석.결과 ①수착저존시간적연장,경형광탐침DCFH-DA부육적거백세포현부홍세포적형광강도현저증강;②저존후제0,7,14,28,42천거백세포현부홍세포제제내ROS함량정현저증고추세,제42천거백세포현부홍세포내ROS함량현저고우제0,7,14,28천ROS함량,병차차이균유통계학의의(P＜0.01,0.05,0.05,0.05);차거백세포현부홍세포제제내ROS함량여저존시간정정상관관계(r=0.898,P＜0.01);③저존후제0,7,14,28,42천거백세포현부홍세포내MDA함량정현저증고추세,제42천거백세포현부홍세포내MDA함량현저고우제0,7,14,28천MDA함량,병차차이균유통계학의의(P＜0.01,0.01,0.01,0.05);차거백세포현부홍세포내MDA함량여저존시간정정상관관계(r=0.930,P＜0.01);④저존후제0,7,14,28,42천,거백세포현부홍세포제제내ROS함량균분별여대응시간점MDA함량정정상관관계(r=0.877,0.872,0.823,0.786,0.907;P＜0.05).거백세포현부홍세포제제저존후제0,7,14,28,42천,각시간점ROS함량균치여MDA함량균치역정정상관성관계(r=0.981,P＜0.01).결론 거백세포현부홍세포제제저존과정중ROS함량여MDA함량균현저증고,홍세포내양화응격반응증강,기위도치거백세포현부홍세포제제저존손상적중요원인.
Objective To investigate the changes of reactive oxygen species (ROS) concentration and malonaldehyde (MDA) concentration in suspended red blood cells without leukocyte during storage.Methods From May 2013 to June 2013,6 bags of suspended red blood cells without leukocyte which were prepared at Hebei Blood Center were collected in this study by random sampling method.On day 0,day 7,day 14,day 28 and day 42 of storage,the expression of ROS in suspended red blood cells without leukocyte were observed by fluorescent probe 2',7'-dichlorofluorescin diacetate (DCFH-DA) incubation method,the ROS concentration and MDA concentration in suspended red blood cells without leukocyte were also detected.Correlation analysis between the ROS concentration,MDA concentration in suspended red blood cells without leukocyte and storage time was carried out,respectively.Correlation analysis between the MDA concentration and ROS concentration in suspended red blood cells without leukocyte after storage was carried out.Results ① With the extension of storage time,fluorescence intensity of suspended red blood cells without leukocyte incubated with fluorescent probe DCFH-DA was significantly enhanced.② On day 0,day 7,day 14,day 28 and day 42 after storage,ROS concentration in suspended red blood cells without leukocyte showed a significant increasing trend.Concentration of ROS in suspended red blood cells without leukocyte on day 42 was significantly higher than that of day 0,day 7,day 14 and day 28 (P＜0.01,0.05,0.05,0.05).There was a positive correlation between the ROS concentration in suspended red blood cells without leukocyte and storage time(r=0.898,P＜0.01).③ On day 0,day 7,day 14,day 28and day 42 after storage,MDA concentration in suspended red blood cells without leukocyte showed a significant increasing trend,MDA concentration in suspended red blood cells without leukocyte on day 42 was significantly higher than that of day 0,day 7,day 14 and day 28 (P＜0.01,0.01,0.01,0.05).There was a positive correlation between the MDA concentration in suspended red blood cells without leukocyte and storage time (r=0.930,P＜0.01).④ On day 0,day 7,day 14,day 28 and day 42 after storage,there was a positive correlation between the ROS concentration in suspended red blood cells without leukocyte and MDA concentration of corresponding time respectively (r=0.877,0.872,0.823,0.786,0.907; P＜0.05).There was also a positive correlation between the mean of ROS concentration of each day and the mean of MDA concentration on day 0,day 7,day 14,day 28 and day 42 after storage (r=0.981,P＜0.01).Conclusions Concentration of ROS and MDA in suspended red blood cells without leukocyte were significantly increased during storage,and oxidative stress reaction was enhanced in red blood cells.This was the important cause leading to the storage lesions of suspended red blood cells without leukocyte.
