国际内分泌代谢杂志
國際內分泌代謝雜誌
국제내분비대사잡지
INTERNATIONAL JOURNAL OF ENDOCRINOLOGY AND METABOLISM
2015年
1期
6-10
,共5页
袁捷%姜云生%杜彦丽%王肃
袁捷%薑雲生%杜彥麗%王肅
원첩%강운생%두언려%왕숙
糖尿病%早孕%子宫肌层%增殖细胞核抗原
糖尿病%早孕%子宮肌層%增殖細胞覈抗原
당뇨병%조잉%자궁기층%증식세포핵항원
Diabetes mellitus%Early pregnancy%Myometrium%Proliferating cell nuclear antigen
目的 探讨1型糖尿病对小鼠早孕子宫肌层结构和细胞增殖的影响.方法 实验中共有7周龄Balb/C雌性小鼠73只,雄性小鼠20只.利用随机数字表法从雌性小鼠中随机取45只于尾静脉注射四氧嘧啶生理盐水溶液诱导1型糖尿病,其余28只作为对照组.共成功诱导1型糖尿病小鼠模型30只,根据随机数字表法选择28只作为糖尿病组.糖尿病组根据病程分为D1组(90～100 d)、D2组(101～ 110 d).于孕168 h,取小鼠子宫肌层通过光镜、电镜下观察组织学病理变化;用免疫组织化学方法检测子宫肌层细胞增殖细胞核抗原(PCNA)的表达.建模前、后检测血糖、胰岛素;观察体重、饮水、饮食等.结果 成功构建了能自然交配并妊娠的糖尿病小鼠.与对照组相比,D1、D2组子宫内、外肌层细胞的增殖指数都明显降低(t=8.15,11.60,P均＜0.001).同时,D1组全肌层厚度及内/外肌层间距均较对照组增大(t=2.12,4.40,P均＜0.05),而D2组全肌层厚度及内/外肌层间距均较对照组明显减小(t=19.93,15.15,P均＜0.001).D1组(t=2.25,3.46,P均＜0.05)、D2组(t=3.46,10.28,P均＜0.001)内、外肌层厚度较对照组减小.D1与D2组的内肌层和外肌层细胞的排列明显打乱.结论 糖尿病对小鼠早孕时期子宫肌层产生不良影响.
目的 探討1型糖尿病對小鼠早孕子宮肌層結構和細胞增殖的影響.方法 實驗中共有7週齡Balb/C雌性小鼠73隻,雄性小鼠20隻.利用隨機數字錶法從雌性小鼠中隨機取45隻于尾靜脈註射四氧嘧啶生理鹽水溶液誘導1型糖尿病,其餘28隻作為對照組.共成功誘導1型糖尿病小鼠模型30隻,根據隨機數字錶法選擇28隻作為糖尿病組.糖尿病組根據病程分為D1組(90～100 d)、D2組(101～ 110 d).于孕168 h,取小鼠子宮肌層通過光鏡、電鏡下觀察組織學病理變化;用免疫組織化學方法檢測子宮肌層細胞增殖細胞覈抗原(PCNA)的錶達.建模前、後檢測血糖、胰島素;觀察體重、飲水、飲食等.結果 成功構建瞭能自然交配併妊娠的糖尿病小鼠.與對照組相比,D1、D2組子宮內、外肌層細胞的增殖指數都明顯降低(t=8.15,11.60,P均＜0.001).同時,D1組全肌層厚度及內/外肌層間距均較對照組增大(t=2.12,4.40,P均＜0.05),而D2組全肌層厚度及內/外肌層間距均較對照組明顯減小(t=19.93,15.15,P均＜0.001).D1組(t=2.25,3.46,P均＜0.05)、D2組(t=3.46,10.28,P均＜0.001)內、外肌層厚度較對照組減小.D1與D2組的內肌層和外肌層細胞的排列明顯打亂.結論 糖尿病對小鼠早孕時期子宮肌層產生不良影響.
목적 탐토1형당뇨병대소서조잉자궁기층결구화세포증식적영향.방법 실험중공유7주령Balb/C자성소서73지,웅성소서20지.이용수궤수자표법종자성소서중수궤취45지우미정맥주사사양밀정생리염수용액유도1형당뇨병,기여28지작위대조조.공성공유도1형당뇨병소서모형30지,근거수궤수자표법선택28지작위당뇨병조.당뇨병조근거병정분위D1조(90～100 d)、D2조(101～ 110 d).우잉168 h,취소서자궁기층통과광경、전경하관찰조직학병리변화;용면역조직화학방법검측자궁기층세포증식세포핵항원(PCNA)적표체.건모전、후검측혈당、이도소;관찰체중、음수、음식등.결과 성공구건료능자연교배병임신적당뇨병소서.여대조조상비,D1、D2조자궁내、외기층세포적증식지수도명현강저(t=8.15,11.60,P균＜0.001).동시,D1조전기층후도급내/외기층간거균교대조조증대(t=2.12,4.40,P균＜0.05),이D2조전기층후도급내/외기층간거균교대조조명현감소(t=19.93,15.15,P균＜0.001).D1조(t=2.25,3.46,P균＜0.05)、D2조(t=3.46,10.28,P균＜0.001)내、외기층후도교대조조감소.D1여D2조적내기층화외기층세포적배렬명현타란.결론 당뇨병대소서조잉시기자궁기층산생불량영향.
Objective To explore the effects of type 1 diabetes on uterine muscularis structure and cell proliferation in mice during early pregnancy.Methods There were 73 female and 20 male Balb/C mice at 7-week age in the test.Forty five female mice were selected according to random number table and injected with alloxan via tail vein to induce type 1 diabetes,and the remaining 28 mice were used as control group.Thirty type 1 diabetic mice were obtained,and 28 mice were selected according to random number table as diabetic group.Then all diabetic mice were divided into two group according to diabetic duration:D1 group (90-100 d) and D2 group (101-110 d) After pregnancy for 168 h,the changes of uterine muscle were examined by light microscope and electron microscope.Immunohistochemistry was used to detect the expression of proliferating cell nuclear antigen(PCNA) in myometrial cells.Blood glucose and insulin,water consumption,diet consumption,and weight were measured before and after the modeling.Results Natural mating and pregnant diabetic mice were successfully induced.Compared with control group,the cell proliferation of inner and outer muscular layer were significant decreased in D1 and D2 group(t =8.15-11.60,all P ＜ 0.001),the total thickness of the muscular layer,the spacing of inner and outer muscular layer were increased(t =2.12,4.40,all P ＜ 0.05) in D1 group,while the total thickness of the musclar layer and the spacing of inner and outer muscular layer was significantly decreased in D2 group(t =19.93,15.15,all P ＜ 0.001).Compared with control group,the thickness of inner muscular layer and outer muscular layer were also decreased in D1 group (t =2.25,3.46,all P ＜ 0.05) and D2 group (t =3.46,10.28,all P ＜ 0.001).And the ultrastructure of inner and outer muscular layer was also changed in D1 group and D2 group.Conclusion Diabetes could cause adverse effects to uterine muscular layer in early pregnant mice.
