国际麻醉学与复苏杂志
國際痳醉學與複囌雜誌
국제마취학여복소잡지
INTERNATIONAL JOURNAL OF ANESTHESIOLOGY AND RESUSCITATION
2015年
1期
31-34
,共4页
孔双明%申文%胡学铭%刘彦楠%张婷%曹守斌
孔雙明%申文%鬍學銘%劉彥楠%張婷%曹守斌
공쌍명%신문%호학명%류언남%장정%조수빈
骨癌痛%磷酸化核转录因子-κB%脊髓背角%星形胶质细胞
骨癌痛%燐痠化覈轉錄因子-κB%脊髓揹角%星形膠質細胞
골암통%린산화핵전록인자-κB%척수배각%성형효질세포
Bone cancer pain%phospho-nuclear factor-κB%Spinal dorsal horn%Astrocyte
目的 观察骨癌痛大鼠脊髓水平磷酸化核转录因子κB(phospho-nuclear factor-κB,p-NF-κB)的表达位置及表达变化. 方法 雌性未交配Sprague-Dawley(SD)大鼠48只,体重160 g～200 g,采用随机数字表法将其随机分为两组:假手术组(S组)、骨癌痛(bone cancer pain,BCP)组(BP组),每组24只.选用yon Frey纤维丝检测各组大鼠造模前及造模后3、7、14、21 d机械缩足阈值(mechanical withdrawal threshold,MWT),评价疼痛行为学变化.采用免疫荧光法检测S组和BP组术前ld及术后7、14、21 d大鼠脊髓L4-6节段背角Ⅰ、Ⅱ板层p-NF-κB阳性细胞数的变化.免疫荧光双标法检测脊髓L4-6节段背角Ⅰ、Ⅱ板层p-NF-κB与神经元标志物神经元特异核蛋白(neuronal nuclei,NeuN)、小胶质细胞标志物离子钙接头蛋白分子-1(ionized calcium binding adaptor molecule-1,Iba-1)、星形胶质细胞标志物胶质纤维酸性蛋白(glia fibrillary acidic protein,GFAP)共表达情况. 结果 各组肿瘤细胞接种前MWT比较差异无统计学意义(P＞0.05);与S组比较,BP组肿瘤细胞接种后第7天MWT[(3.57±1.29)g]、第14天MWT[(1.66±0.39)g]、第21天MWT[(1.40±0.16)g]降低(P＜0.05);S组各时间点MWT差异无统计学意义(P＞0.05).与S组比较,BP组大鼠术后7d脊髓L4-6节段背角Ⅰ、Ⅱ板层p-NF-κB表达水平升高,随时间延长逐渐升高(P＜0.05).p-NF-κB几乎都与GFAP共表达,而很少与NeuN、Iba-1共表达. 结论 BCP大鼠脊髓背角水平星形胶质细胞内p-NF-κB参与介导BCP大鼠机械痛敏.
目的 觀察骨癌痛大鼠脊髓水平燐痠化覈轉錄因子κB(phospho-nuclear factor-κB,p-NF-κB)的錶達位置及錶達變化. 方法 雌性未交配Sprague-Dawley(SD)大鼠48隻,體重160 g～200 g,採用隨機數字錶法將其隨機分為兩組:假手術組(S組)、骨癌痛(bone cancer pain,BCP)組(BP組),每組24隻.選用yon Frey纖維絲檢測各組大鼠造模前及造模後3、7、14、21 d機械縮足閾值(mechanical withdrawal threshold,MWT),評價疼痛行為學變化.採用免疫熒光法檢測S組和BP組術前ld及術後7、14、21 d大鼠脊髓L4-6節段揹角Ⅰ、Ⅱ闆層p-NF-κB暘性細胞數的變化.免疫熒光雙標法檢測脊髓L4-6節段揹角Ⅰ、Ⅱ闆層p-NF-κB與神經元標誌物神經元特異覈蛋白(neuronal nuclei,NeuN)、小膠質細胞標誌物離子鈣接頭蛋白分子-1(ionized calcium binding adaptor molecule-1,Iba-1)、星形膠質細胞標誌物膠質纖維痠性蛋白(glia fibrillary acidic protein,GFAP)共錶達情況. 結果 各組腫瘤細胞接種前MWT比較差異無統計學意義(P＞0.05);與S組比較,BP組腫瘤細胞接種後第7天MWT[(3.57±1.29)g]、第14天MWT[(1.66±0.39)g]、第21天MWT[(1.40±0.16)g]降低(P＜0.05);S組各時間點MWT差異無統計學意義(P＞0.05).與S組比較,BP組大鼠術後7d脊髓L4-6節段揹角Ⅰ、Ⅱ闆層p-NF-κB錶達水平升高,隨時間延長逐漸升高(P＜0.05).p-NF-κB幾乎都與GFAP共錶達,而很少與NeuN、Iba-1共錶達. 結論 BCP大鼠脊髓揹角水平星形膠質細胞內p-NF-κB參與介導BCP大鼠機械痛敏.
목적 관찰골암통대서척수수평린산화핵전록인자κB(phospho-nuclear factor-κB,p-NF-κB)적표체위치급표체변화. 방법 자성미교배Sprague-Dawley(SD)대서48지,체중160 g～200 g,채용수궤수자표법장기수궤분위량조:가수술조(S조)、골암통(bone cancer pain,BCP)조(BP조),매조24지.선용yon Frey섬유사검측각조대서조모전급조모후3、7、14、21 d궤계축족역치(mechanical withdrawal threshold,MWT),평개동통행위학변화.채용면역형광법검측S조화BP조술전ld급술후7、14、21 d대서척수L4-6절단배각Ⅰ、Ⅱ판층p-NF-κB양성세포수적변화.면역형광쌍표법검측척수L4-6절단배각Ⅰ、Ⅱ판층p-NF-κB여신경원표지물신경원특이핵단백(neuronal nuclei,NeuN)、소효질세포표지물리자개접두단백분자-1(ionized calcium binding adaptor molecule-1,Iba-1)、성형효질세포표지물효질섬유산성단백(glia fibrillary acidic protein,GFAP)공표체정황. 결과 각조종류세포접충전MWT비교차이무통계학의의(P＞0.05);여S조비교,BP조종류세포접충후제7천MWT[(3.57±1.29)g]、제14천MWT[(1.66±0.39)g]、제21천MWT[(1.40±0.16)g]강저(P＜0.05);S조각시간점MWT차이무통계학의의(P＞0.05).여S조비교,BP조대서술후7d척수L4-6절단배각Ⅰ、Ⅱ판층p-NF-κB표체수평승고,수시간연장축점승고(P＜0.05).p-NF-κB궤호도여GFAP공표체,이흔소여NeuN、Iba-1공표체. 결론 BCP대서척수배각수평성형효질세포내p-NF-κB삼여개도BCP대서궤계통민.
Objective To investigate the expression of spinal phospho-nuclear factor-κB (p-NF-κB) in a rat model of tibial bone cancer pain.Methods Forty-eight female Sprague-Dawley (SD) rats weighting 160 g-200 g were randomly divided into two groups:sham operation group (group S) and bone cancer pain(BCP) group(group BP).Before operation and at 3,7,14,21 d after operation,changes of pain behavior of rats in each group were measured using yon Frey filaments.The rats were sacrificed on one day before and 7,14,21 d after operation and L4-6 segments of the spinal cords were removed for measurement of the expression of p-NF-κB protein by fluorescent immunohistochemistry.Double immunostsining for p-NF κB with cell-specific markers:neuronal nuclei (NeuN),glia fibrillary acidic protein (GFAP) and ionized calcium binding adaptor molecule-1 (Iba-1) was performed to identify the cell types that expressed p-NF-κB.Results Behavioral assays reveals that the mechanical withdrawal threshold (MWT) was significantly decreased at 7 d [(3.57±1.29) g],14 d [(1.66±0.39) g],21 d [(1.40±0.16) g] after operation (P＜0.05) and decreased gradually with the progress of the disease in the group BP.Compared with that in group S,the expression of p-NF-κB was significantly increased at 7 d after operation (P＜0.05) and increased gradually afterwards in the group BP.p-NF-κB was primarily coexpressed with GFAP,but little with NeuN and Iba-1 of group BP rats.Conclusions The expression of p-NF-κB in spinal astrocytes was involved in the development of tibial bone cancer pain.
