国际流行病学传染病学杂志
國際流行病學傳染病學雜誌
국제류행병학전염병학잡지
INTERNATIONAL JOURNAL OF EPIDEMIOLOGY AND INFECTIOUS DISEASE
2014年
6期
378-381
,共4页
结核,肺%Th17细胞%调节性T细胞%免疫功能
結覈,肺%Th17細胞%調節性T細胞%免疫功能
결핵,폐%Th17세포%조절성T세포%면역공능
Tuberculosis,pulmonary%Th17 cell%Regulation T cell%Immunologic function
目的 探讨外周血辅助性T细胞17(Th17)和调节性T细胞(Treg)在调节初治肺结核患者免疫功能中的作用.方法 选取2012年1月至2013年6月在绍兴市第六人民医院就诊的初治肺结核患者169例,根据有无空洞分成空洞组(73例)和无空洞组(96例);根据空洞所占肺野将空洞组的73例患者分成1～2个肺野组、3～4个肺野组和5～6个肺野组.另外选取同期健康体格检查者51名作为健康对照组.流式细胞仪动态检测治疗前、治疗2个月时患者外周血CD4+T细胞上Th17和Treg细胞水平,并比较各组间Th17细胞和Treg细胞水平的差异.结果 肺结核组治疗前及治疗2个月后外周血Th17细胞表达水平分别为(1.63±0.38)％和(2.57±0.82)％,均低于健康对照组的(3.85±1.13)％,差异有统计学意义(t=4.18、2.69,P＜0.01);外周血Treg细胞表达水平分别为(7.53±1.77)％和(5.39±1.22)％,显著高于健康对照组的(3.99±0.84)％,差异有统计学意义(t=6.27、3.34,P＜0.01).空洞组Th17的细胞的表达水平为(1.49±0.25)％,低于无空洞组的(1.87±0.46)％;Treg细胞水平为(7.94±1.85)％,高于无空洞型组的(6.88±1.14)％,组间差异均有统计学意义(t=2.35、2.59,P＜0.05).1～2个肺野组、3～4个肺野组和5～6个肺野组间Th17细胞水平随着空洞数量增加而降低,但Treg细胞逐渐升高,组间差异有统计学意义(F=3.10、3.82,P＜ 0.05).结论 外周血Th17和Treg细胞水平与肺结核的严重程度密切相关,对结核免疫反应起重要调节作用.
目的 探討外週血輔助性T細胞17(Th17)和調節性T細胞(Treg)在調節初治肺結覈患者免疫功能中的作用.方法 選取2012年1月至2013年6月在紹興市第六人民醫院就診的初治肺結覈患者169例,根據有無空洞分成空洞組(73例)和無空洞組(96例);根據空洞所佔肺野將空洞組的73例患者分成1～2箇肺野組、3～4箇肺野組和5～6箇肺野組.另外選取同期健康體格檢查者51名作為健康對照組.流式細胞儀動態檢測治療前、治療2箇月時患者外週血CD4+T細胞上Th17和Treg細胞水平,併比較各組間Th17細胞和Treg細胞水平的差異.結果 肺結覈組治療前及治療2箇月後外週血Th17細胞錶達水平分彆為(1.63±0.38)％和(2.57±0.82)％,均低于健康對照組的(3.85±1.13)％,差異有統計學意義(t=4.18、2.69,P＜0.01);外週血Treg細胞錶達水平分彆為(7.53±1.77)％和(5.39±1.22)％,顯著高于健康對照組的(3.99±0.84)％,差異有統計學意義(t=6.27、3.34,P＜0.01).空洞組Th17的細胞的錶達水平為(1.49±0.25)％,低于無空洞組的(1.87±0.46)％;Treg細胞水平為(7.94±1.85)％,高于無空洞型組的(6.88±1.14)％,組間差異均有統計學意義(t=2.35、2.59,P＜0.05).1～2箇肺野組、3～4箇肺野組和5～6箇肺野組間Th17細胞水平隨著空洞數量增加而降低,但Treg細胞逐漸升高,組間差異有統計學意義(F=3.10、3.82,P＜ 0.05).結論 外週血Th17和Treg細胞水平與肺結覈的嚴重程度密切相關,對結覈免疫反應起重要調節作用.
목적 탐토외주혈보조성T세포17(Th17)화조절성T세포(Treg)재조절초치폐결핵환자면역공능중적작용.방법 선취2012년1월지2013년6월재소흥시제륙인민의원취진적초치폐결핵환자169례,근거유무공동분성공동조(73례)화무공동조(96례);근거공동소점폐야장공동조적73례환자분성1～2개폐야조、3～4개폐야조화5～6개폐야조.령외선취동기건강체격검사자51명작위건강대조조.류식세포의동태검측치료전、치료2개월시환자외주혈CD4+T세포상Th17화Treg세포수평,병비교각조간Th17세포화Treg세포수평적차이.결과 폐결핵조치료전급치료2개월후외주혈Th17세포표체수평분별위(1.63±0.38)％화(2.57±0.82)％,균저우건강대조조적(3.85±1.13)％,차이유통계학의의(t=4.18、2.69,P＜0.01);외주혈Treg세포표체수평분별위(7.53±1.77)％화(5.39±1.22)％,현저고우건강대조조적(3.99±0.84)％,차이유통계학의의(t=6.27、3.34,P＜0.01).공동조Th17적세포적표체수평위(1.49±0.25)％,저우무공동조적(1.87±0.46)％;Treg세포수평위(7.94±1.85)％,고우무공동형조적(6.88±1.14)％,조간차이균유통계학의의(t=2.35、2.59,P＜0.05).1～2개폐야조、3～4개폐야조화5～6개폐야조간Th17세포수평수착공동수량증가이강저,단Treg세포축점승고,조간차이유통계학의의(F=3.10、3.82,P＜ 0.05).결론 외주혈Th17화Treg세포수평여폐결핵적엄중정도밀절상관,대결핵면역반응기중요조절작용.
Objective To investigate the peripheral T help 17 cells (Th17) and regulatory T cell (Treg) role in regulating the immunologic function of tuberculosis patients undergoing initial treatment.Methods One hundred and sixty-nine tuberculosis patients with initial treatment in Shaoxing Sixth People's Hospital from January 2012 to July 2013 were enrolled in the study.They were divided into two groups:patients with cavitations (n=73) and without cavitation (n=96).Patients with cavitations were divided into 1-2 lung fields affected subgroup,3-4 lung fields affected subgroup and 5-6 lung fields affected subgroup.Fifty-one healthy individuals were selected as control group.The percentages of Th17 and Treg on CD4+ T cells in peripheral blood were tested by flow cytometry at the time of pre-therapy and 2 months later.Results The levels of peripheral Th17 in tuberculosis patients were (1.63±0.38)％ and (2.57±0.82)％ at the start of treatment and 2 months later,respectively,which were significantly lower than control group of (3.85±1.13)％ (t =4.18,2.69,P both＜0.01).The levels of Treg in tuberculosis patients were (7.53±1.77)％ and (5.39±1.12)％,which were significantly higher than control group of (3.99±0.84)％(t=6.27,3.34,P both＜0.01).Th17 level in patients with cavitions was lower than that in patients without cavitation[(1.49±0.25)％ vs(1.87±0.46)％],while Treg level was higher in patients with cavitations than in patients without cavitation [(7.94±1.85)％ vs(6.88±1.14)％],both with significant differences(t=2.35,2.59,P both＜0.05).The expression rate of Th17 decreased as cavitation area increased,while a reverse pattern was seen in the level of Treg,and significant differences were found among 3 different lung field affected groups(F=3.10,3.82,P＜0.05).Conclusions The expression rates of Th17 and Treg are correlated with tuberculosis disease progression.They can play important roles in immune function of patients with tuberculosis.
