CAJ | 학술논문

光声流式细胞技术(PAFC)是在整合了高重复率激光技术、信号快速获取技术、超声聚焦转换等技术的基础上发展起来的新型体内细胞检测技术.其原理为采用低功率、高重复率的激光在特定波长下进行照射,检测目标物接受特定波长的激光激发后产生快速激光能量的弛豫,发射出声波信号被接收.PAFC具有高效、无创、实时的特点,使实时动态监测循环或淋巴系统中肿瘤细胞的愿望逐渐成为现实,是一种极具前景的临床肿瘤研究工具.文章介绍PAFC的原理、近年来的发展应用及目前尚待解决的问题.
광성류식세포기술(PAFC)시재정합료고중복솔격광기술、신호쾌속획취기술、초성취초전환등기술적기출상발전기래적신형체내세포검측기술.기원리위채용저공솔、고중복솔적격광재특정파장하진행조사,검측목표물접수특정파장적격광격발후산생쾌속격광능량적이예,발사출성파신호피접수.PAFC구유고효、무창、실시적특점,사실시동태감측순배혹림파계통중종류세포적원망축점성위현실,시일충겁구전경적림상종류연구공구.문장개소PAFC적원리、근년래적발전응용급목전상대해결적문제.
Photoacoustic flow cytometry (PAFC) is a novel flow cytometry which integrates high-pulse repetition-rate lasers,fast signal acquisition algorithms and focused ultrasound transducers to assess deep vessels.The technical principle is that the cells in blood or lymph flow are irradiated with several laser beams with different wavelengths,then laser-induced PT effects are detected by corresponding schematics.PAFC is characterized by its high efficiency,no invasion and real-time detection,which makes it possible to detect tumor cells in circulation or in lymphatic system dynamically and in real time.So for,it is considered as one of the most promising techniques in cancer research.This article will address the principle,application and several problems of PAFC.