肿瘤研究与临床
腫瘤研究與臨床
종류연구여림상
CANCER RESEARCH AND CLINIC
2014年
12期
801-804
,共4页
吴小兵%徐学虎%伍尚标%江庆萍%刘海波%陈戎%孙嫣
吳小兵%徐學虎%伍尚標%江慶萍%劉海波%陳戎%孫嫣
오소병%서학호%오상표%강경평%류해파%진융%손언
结直肠肿瘤%miR-125a-5p%分化程度%诊断
結直腸腫瘤%miR-125a-5p%分化程度%診斷
결직장종류%miR-125a-5p%분화정도%진단
Colorectal neoplasms%miR-125a-5p%Differentiation stage%Diagnosis
目的 分析研究miR-125a-5p在不同分化程度结直肠癌(CRC)中的表达水平,探讨其对不同分化CRC的诊断意义.方法 采用实时荧光定量PCR分析miR-125a-5p在26例不同分化程度CRC患者肿瘤和瘤旁组织中的表达水平,比较miR-125a-5p在肿瘤和瘤旁组织中表达水平的差异.通过受试者特征曲线(ROC)分析miR-125a-5p作为诊断标志筛查CRC患者的特异性和敏感性.结果 相对瘤旁正常组织,miR-125a-5p在高、中分化CRC组织中的表达水平均明显下降,其中在高分化肿瘤样本中的表达水平下降了近300％(P=0.047),在中分化CRC中下降超过500％(P=0.000).miR-125a-5p对筛查中分化CRC具有较高的敏感度和特异度,分别达到80.00％、90.00％.结论 不同分化程度CRC中miR-125a-5p表达水平均明显下降,提示miR-125a-5p在CRC的分化发展中具有重要作用.miR-125a-5p具有作为CRC临床筛查指标的潜能.
目的 分析研究miR-125a-5p在不同分化程度結直腸癌(CRC)中的錶達水平,探討其對不同分化CRC的診斷意義.方法 採用實時熒光定量PCR分析miR-125a-5p在26例不同分化程度CRC患者腫瘤和瘤徬組織中的錶達水平,比較miR-125a-5p在腫瘤和瘤徬組織中錶達水平的差異.通過受試者特徵麯線(ROC)分析miR-125a-5p作為診斷標誌篩查CRC患者的特異性和敏感性.結果 相對瘤徬正常組織,miR-125a-5p在高、中分化CRC組織中的錶達水平均明顯下降,其中在高分化腫瘤樣本中的錶達水平下降瞭近300％(P=0.047),在中分化CRC中下降超過500％(P=0.000).miR-125a-5p對篩查中分化CRC具有較高的敏感度和特異度,分彆達到80.00％、90.00％.結論 不同分化程度CRC中miR-125a-5p錶達水平均明顯下降,提示miR-125a-5p在CRC的分化髮展中具有重要作用.miR-125a-5p具有作為CRC臨床篩查指標的潛能.
목적 분석연구miR-125a-5p재불동분화정도결직장암(CRC)중적표체수평,탐토기대불동분화CRC적진단의의.방법 채용실시형광정량PCR분석miR-125a-5p재26례불동분화정도CRC환자종류화류방조직중적표체수평,비교miR-125a-5p재종류화류방조직중표체수평적차이.통과수시자특정곡선(ROC)분석miR-125a-5p작위진단표지사사CRC환자적특이성화민감성.결과 상대류방정상조직,miR-125a-5p재고、중분화CRC조직중적표체수평균명현하강,기중재고분화종류양본중적표체수평하강료근300％(P=0.047),재중분화CRC중하강초과500％(P=0.000).miR-125a-5p대사사중분화CRC구유교고적민감도화특이도,분별체도80.00％、90.00％.결론 불동분화정도CRC중miR-125a-5p표체수평균명현하강,제시miR-125a-5p재CRC적분화발전중구유중요작용.miR-125a-5p구유작위CRC림상사사지표적잠능.
Objective To identify the expression level of miR-125a-5p in colorectal cancer (CRC) with various differentiation,and to investigate its diagnostic significance.Methods Real-time polymerase chain reaction was used to analyze the expression level of miR-125a-5p in tumors and paired normal tissues of different differentiated CRC.Receiver-operating-characteristic (ROC) curve was performed to evaluate the specificity and sensitivity of using miR-125a-5p as biomarkers to discriminate CRC patients from normal persons.Results Compared with normal tissues,miR-125a-5p was down-regulated in both high and moderate differentiation,miR-125a-5p could distinguish CRC from normal persons with high sensitivity and specificity,which were 80.00 ％ and 90.00 ％,respectively.Conclusions miR-125a-5p down-regulated in CRC indicates that miR-125a-5p contributes to tumorigenesis,and may be a potential biomarker for CRC.
