中华整形外科杂志
中華整形外科雜誌
중화정형외과잡지
CHINESE JOURNAL OF PLASTIC SURGERY
2015年
1期
53-57
,共5页
李丹%惠瑞%胡咏武%韩岩%郭树忠
李丹%惠瑞%鬍詠武%韓巖%郭樹忠
리단%혜서%호영무%한암%곽수충
血竭%伤口愈合%成纤维细胞%细胞增殖%透明质酸
血竭%傷口愈閤%成纖維細胞%細胞增殖%透明質痠
혈갈%상구유합%성섬유세포%세포증식%투명질산
Dragon's blood%Wound healing%Fibroblast%Cell proliferation%Hyaluronic acid
目的 研究血竭提取物对成纤维细胞生物学作用的影响.方法 将血竭经过氯仿、乙酸乙酯、乙醇依次回流提取分别得到3种血竭提取液,培养液稀释后行成纤维细胞培养.噻唑蓝法MTT检测浓度分别为0.002、0.02、0.2、2、20 mg/ml的血竭提取物,在0、12、24、36、48、60、72h7个检测点对体外培养成纤维细胞增殖的影响,并绘制最适浓度下细胞生长曲线.流式细胞术FCM分析最适浓度培养下成纤维细胞的细胞周期变化.应用放射免疫分析法检测0、12、24、36、48、60、72 h细胞培养上清液中透明质酸(hyaluronic acid,HA)含量.结果 血竭乙酸乙酯提取物在0.2～2 mg/ml浓度范围内,促进成纤维细胞增殖,且呈浓度依赖性,在2 mg/ml浓度值时促进作用最显著,处于S期的细胞[(25.80±3.10)％]较对照组[(7.50±0.70)％]显著增加(P＜0.01),在此条件下培养成纤维细胞,12～72 h实验组分泌量逐渐增加,但较对照组偏低,组间比较差异有统计学意义(P＜0.01).结论 血竭乙酸乙酯提取物具有显著促进成纤维细胞增殖作用,可能是血竭促进创面愈合的机制之一.
目的 研究血竭提取物對成纖維細胞生物學作用的影響.方法 將血竭經過氯倣、乙痠乙酯、乙醇依次迴流提取分彆得到3種血竭提取液,培養液稀釋後行成纖維細胞培養.噻唑藍法MTT檢測濃度分彆為0.002、0.02、0.2、2、20 mg/ml的血竭提取物,在0、12、24、36、48、60、72h7箇檢測點對體外培養成纖維細胞增殖的影響,併繪製最適濃度下細胞生長麯線.流式細胞術FCM分析最適濃度培養下成纖維細胞的細胞週期變化.應用放射免疫分析法檢測0、12、24、36、48、60、72 h細胞培養上清液中透明質痠(hyaluronic acid,HA)含量.結果 血竭乙痠乙酯提取物在0.2～2 mg/ml濃度範圍內,促進成纖維細胞增殖,且呈濃度依賴性,在2 mg/ml濃度值時促進作用最顯著,處于S期的細胞[(25.80±3.10)％]較對照組[(7.50±0.70)％]顯著增加(P＜0.01),在此條件下培養成纖維細胞,12～72 h實驗組分泌量逐漸增加,但較對照組偏低,組間比較差異有統計學意義(P＜0.01).結論 血竭乙痠乙酯提取物具有顯著促進成纖維細胞增殖作用,可能是血竭促進創麵愈閤的機製之一.
목적 연구혈갈제취물대성섬유세포생물학작용적영향.방법 장혈갈경과록방、을산을지、을순의차회류제취분별득도3충혈갈제취액,배양액희석후행성섬유세포배양.새서람법MTT검측농도분별위0.002、0.02、0.2、2、20 mg/ml적혈갈제취물,재0、12、24、36、48、60、72h7개검측점대체외배양성섬유세포증식적영향,병회제최괄농도하세포생장곡선.류식세포술FCM분석최괄농도배양하성섬유세포적세포주기변화.응용방사면역분석법검측0、12、24、36、48、60、72 h세포배양상청액중투명질산(hyaluronic acid,HA)함량.결과 혈갈을산을지제취물재0.2～2 mg/ml농도범위내,촉진성섬유세포증식,차정농도의뢰성,재2 mg/ml농도치시촉진작용최현저,처우S기적세포[(25.80±3.10)％]교대조조[(7.50±0.70)％]현저증가(P＜0.01),재차조건하배양성섬유세포,12～72 h실험조분비량축점증가,단교대조조편저,조간비교차이유통계학의의(P＜0.01).결론 혈갈을산을지제취물구유현저촉진성섬유세포증식작용,가능시혈갈촉진창면유합적궤제지일.
Objective To investigate the effects of Dragon' s blood extract on proliferation and secret extracellular matrix function of fibroblasts in vitro.Methods Dragon ' s blood was extracted by chloroform,acetoacetic ester,alcohol.Human fibroblast were cultured in vitro in media containing gradient dilutions of Dragon's blood extracts (0.002,0.02,0.2,2,20 mg/ml),which was followed by cell proliferation assessed with MTT assay on 0,12,24,36,48,60,72 h.Under the optimal concentration,the cell growth curves were drawn and the flow cytometry (FCM) was used to determine the changes of cell cycle.On 0,12,24,36,48,60,72 h,the concentration of hyaluronic acid in the supernatant of fibroblast culture was measured by radioimmunoassay.Results 0.2-2 mg/ml Dragon' s blood extracts enhanced the proliferation of fibroblasts in a dose-dependent manner.2 mg/ml was the optimal dilution of Dragon' s blood extract,and it increased the ratio of S cells in cell cycle[(25.80 ± 3.10) ％] than control group[(7.50 ± 0.70)％,P ＜ 0.01].From 12 h to 72 h,in 2 mg/ml Dragon's blood group,concentration of Hyaluronic acid secreted by fibroblasts gradually increased,but were less than control(P ＜ 0.01).Conclusions Dragon ' s blood acetoacetic ester extract improved the proliferation of cultured human fibroblasts in vitro,might be beneficial to promote wound healing.
