CAJ | 학술논문

目的探讨预注射高渗盐水(7.5％ NaCl)对老龄大鼠术后认知功能的影响.方法健康雄性SD大鼠60只,18月龄,体重450～ 500 g,采用随机数字表法,将其分为3组(n=20):对照组(C组)、模型组(M组)、高渗盐水组(HS组).C组和HS组术前30 min尾静脉注射高渗盐水4 ml/kg,M组注射同容量生理盐水,M组和HS组吸人3％七氟醚麻醉后行脾脏切除术,C组仅吸入纯氧.分别于术前1d,术后第1、3、7天时行Morris水迷宫实验,记录大鼠逃避潜伏期和穿越原平台次数.于术前1d,术后1、7d流式细胞学法测定海马神经元凋亡率、胞质Ca2浓度([Ca2+]i);透射电镜下观察大鼠海马神经元超微结构.结果与术前1d比较,M组和HS组术后各时点逃避潜伏期延长和原平台穿越次数减少,海马神经元凋亡率、[Ca2+]i升高(P＜0.05),C组吸氧后各时点逃避潜伏期、原平台穿越次数、海马神经元凋亡率、[Ca2+]i差异无统计学意义(P＞0.05);与M组比较,HS组术后逃避潜伏期缩短和穿越原平台次数增多,海马神经元凋亡率、[Ca2+]i降低(D＜0.05).M组和HS组海马神经元电镜下出现病理学损伤,M组重于HS组,C组海马神经元电镜下超微结构未见异常.结论预注射7.5％高渗盐水能够改善老龄大鼠术后认知功能,其机制可能与抑制海马神经元凋亡有关.
목적 탐토예주사고삼염수(7.5％ NaCl)대노령대서술후인지공능적영향.방법 건강웅성SD대서60지,18월령,체중450～ 500 g,채용수궤수자표법,장기분위3조(n=20):대조조(C조)、모형조(M조)、고삼염수조(HS조).C조화HS조술전30 min미정맥주사고삼염수4 ml/kg,M조주사동용량생리염수,M조화HS조흡인3％칠불미마취후행비장절제술,C조부흡입순양.분별우술전1d,술후제1、3、7천시행Morris수미궁실험,기록대서도피잠복기화천월원평태차수.우술전1d,술후1、7d류식세포학법측정해마신경원조망솔、포질Ca2농도([Ca2+]i);투사전경하관찰대서해마신경원초미결구.결과 여술전1d비교,M조화HS조술후각시점도피잠복기연장화원평태천월차수감소,해마신경원조망솔、[Ca2+]i승고(P＜0.05),C조흡양후각시점도피잠복기、원평태천월차수、해마신경원조망솔、[Ca2+]i차이무통계학의의(P＞0.05);여M조비교,HS조술후도피잠복기축단화천월원평태차수증다,해마신경원조망솔、[Ca2+]i강저(D＜0.05).M조화HS조해마신경원전경하출현병이학손상,M조중우HS조,C조해마신경원전경하초미결구미견이상.결론 예주사7.5％고삼염수능구개선노령대서술후인지공능,기궤제가능여억제해마신경원조망유관.
Objective To explore the effects of hypertonic saline (7.5％ NaCl) pre-injection on postoperative cognitive function in senile rats.Methods A total of 60 healthy male Sprague-Dawley rats,aged 18 months,weighting 450-500 g,were randomly divided into 3 groups of control (C) ; model (M) and hypertonic saline (HS) (n =20 each).At 30 min pre-operation,the rats of groups C and HS received an injection of 7.5％ hypertonic sodium 4 ml/kg via tail vein.And the same volume of saline was injected in group M.Rats of groups M and HS were anesthetized by inhaling 3％ sevoflourane and underwent splenectomy while group C inhaled merely pure oxygen.Escape latency and frequency of crossing original platform were assessed by Morris water maze on 1 day pre-operation and 1,3,7 days post-operation.The rats were randomly taken for detecting the intracellular [Ca2+] i and apoptotic rate of hippocampal neuron with flow cytometry.And ultrastructures of hippocampal neurons were observed with transmission electron microscope at 1 day pre-operation and 1,7 days post-operation.Results Compared with pre-dosing value,escape lateucy was significantly prolonged,the frequency of crossing original platform decreased and apoptotic rate and [Ca2+]i increased at each timepoint post-operation in groups M and HS.And no significant changes were found in the above-mentioned parameters in group C.Compared with group M,escape latency was significantly shortened,the frequency of crossing original platform increased and apoptotic rate and [Ca2+]i decreased at each timepoint post-operation in group HS.Pathological changes were found in groups M and HS and the damage was more severe in group M than that in group HS.And no significant pathological change was found in group C.Conclusion Infusing 7.5％ hypertonic saline can improve postoperative cognitive function of senile rats.And it may be due to a decreased apoptotic rate of hippocampal neurons.