中华医学杂志
中華醫學雜誌
중화의학잡지
National Medical Journal of China
2015年
3期
221-225
,共5页
黄月%黄普超%任秀花%张杰文
黃月%黃普超%任秀花%張傑文
황월%황보초%임수화%장걸문
CX43半通道蛋白%脑缺血再灌注损伤%星形胶质细胞
CX43半通道蛋白%腦缺血再灌註損傷%星形膠質細胞
CX43반통도단백%뇌결혈재관주손상%성형효질세포
CX43-hemichannel%Ischemia-reperfusion in brain%Astrocytes
目的 观察大鼠脑缺血再灌注损伤后星形胶质细胞CX43半通道对大脑海马区神经元损伤的影响.方法 128只SD雄性大鼠随机数字法分为四组,Sham组,I/R组,I/R+ Gap26组,I/R+NS组,每组32只.用TTC染色法检测各组再灌注24 h后大脑梗死体积;用免疫组化及Western印迹的方法检测脑缺血再灌注损伤后各组胶质原纤维酸性蛋白(GFAP)、星形胶质细胞CX43的表达情况;用尼氏染色及免疫组化来观察大脑海马区神经元死亡的情况.结果 与Sham组相比,I/R组大脑梗死体积[(132±7) mm3比0]增加、星形胶质细胞CX43[(2.45±0.56)比(1.15±0.43)]、GFAP蛋白表达[(146±11)比(76 ±5)]增加(P＜0.05),神经元损伤程度增加;与I/R+ NS组相比,I/R+Gap26大脑梗死体积[(76±6)mm3)比(140±10)mm3]减少、星形胶质细胞CX43[(0.43 ±0.16)比(2.15±0.73)]、GFAP蛋白表达[(57±6)比(140±9)]降低(P＜0.05),神经元损伤程度减轻.结论 阻断CX43半通道可以降低脑缺血再灌注损伤后星形胶质细胞的活化,并且减轻了大脑海马区神经元的损伤.
目的 觀察大鼠腦缺血再灌註損傷後星形膠質細胞CX43半通道對大腦海馬區神經元損傷的影響.方法 128隻SD雄性大鼠隨機數字法分為四組,Sham組,I/R組,I/R+ Gap26組,I/R+NS組,每組32隻.用TTC染色法檢測各組再灌註24 h後大腦梗死體積;用免疫組化及Western印跡的方法檢測腦缺血再灌註損傷後各組膠質原纖維痠性蛋白(GFAP)、星形膠質細胞CX43的錶達情況;用尼氏染色及免疫組化來觀察大腦海馬區神經元死亡的情況.結果 與Sham組相比,I/R組大腦梗死體積[(132±7) mm3比0]增加、星形膠質細胞CX43[(2.45±0.56)比(1.15±0.43)]、GFAP蛋白錶達[(146±11)比(76 ±5)]增加(P＜0.05),神經元損傷程度增加;與I/R+ NS組相比,I/R+Gap26大腦梗死體積[(76±6)mm3)比(140±10)mm3]減少、星形膠質細胞CX43[(0.43 ±0.16)比(2.15±0.73)]、GFAP蛋白錶達[(57±6)比(140±9)]降低(P＜0.05),神經元損傷程度減輕.結論 阻斷CX43半通道可以降低腦缺血再灌註損傷後星形膠質細胞的活化,併且減輕瞭大腦海馬區神經元的損傷.
목적 관찰대서뇌결혈재관주손상후성형효질세포CX43반통도대대뇌해마구신경원손상적영향.방법 128지SD웅성대서수궤수자법분위사조,Sham조,I/R조,I/R+ Gap26조,I/R+NS조,매조32지.용TTC염색법검측각조재관주24 h후대뇌경사체적;용면역조화급Western인적적방법검측뇌결혈재관주손상후각조효질원섬유산성단백(GFAP)、성형효질세포CX43적표체정황;용니씨염색급면역조화래관찰대뇌해마구신경원사망적정황.결과 여Sham조상비,I/R조대뇌경사체적[(132±7) mm3비0]증가、성형효질세포CX43[(2.45±0.56)비(1.15±0.43)]、GFAP단백표체[(146±11)비(76 ±5)]증가(P＜0.05),신경원손상정도증가;여I/R+ NS조상비,I/R+Gap26대뇌경사체적[(76±6)mm3)비(140±10)mm3]감소、성형효질세포CX43[(0.43 ±0.16)비(2.15±0.73)]、GFAP단백표체[(57±6)비(140±9)]강저(P＜0.05),신경원손상정도감경.결론 조단CX43반통도가이강저뇌결혈재관주손상후성형효질세포적활화,병차감경료대뇌해마구신경원적손상.
Objective To explore the CX43-hemichannels effects on rat hippocampus neuron damage after ischemia-reperfusion.Methods A total of 128 Sprague-Dawley male rats were randomly divided into 4 groups of sham-operated group (sham),no treatment (I/R),connexin 43 mimetic peptide (I/R + Gap26) and normal saline (I/R + NS) (n =32 each).TTC staining was used to examine each brain infarct volume after 24 h reperfusion.The levels of connexin 43 and astrocyte activation (glial fibrillary acidic protein) were assessed with Western blot and immunohistochemistry.And neuronal morphology of hippocampus was examined by Nissl stain and immunohistochemistry.Results As compared with sham group,brain infarction volume [(132±7) mm3 vs 0] and the expressions of astrocytic CX43 and GFAP protein in I/R group increased [CX43:(2.45 ± 0.56) vs (1.15 ± 0.43) ; GFAP:(146 ± 11) vs (76 ± 5),P ＜0.05).And the damage of neuron increased versus I/R + NS group,brain infarction volume [(76 ± 6) vs (140 ± 10) mm3] and the expressions of astrocytic CX43 and GFAP were lower in I/R + Gap26 group [CX43:(0.43±0.16) vs (2.15±0.73); GFAP:(57 ±6) vs (140±9),P＜0.05] and neuronal damage lessened.Conclusion Blocking CX43-hemichannel may reduce astrocytic activation and lessen the damage of hippocampus neurons after cerebral ischemia-reperfusion injury.
