中华医学杂志
中華醫學雜誌
중화의학잡지
National Medical Journal of China
2015年
1期
26-29
,共4页
韩怡炜%杨孜%丁晓燕%于欢%易雁鸿
韓怡煒%楊孜%丁曉燕%于歡%易雁鴻
한이위%양자%정효연%우환%역안홍
先兆子痫%脂肪酸类,非酯化%肝%胎盘%胎儿
先兆子癇%脂肪痠類,非酯化%肝%胎盤%胎兒
선조자간%지방산류,비지화%간%태반%태인
Preeclampsia%Long-chain-3-hydroxyacyl-CoA dehydrogenase%Liver%Placenta%Fetus
目的 探讨长链脂肪酸氧化酶LCHAD在妊娠中期子痫前期样改变母鼠和胎鼠组织中的蛋白表达及分布.方法 将C57BL/6J孕鼠于孕1id皮下注射一氧化氮合酶抑制剂建立子痫前期样模型,同时设立注射生理盐水的正常妊娠对照组,模型鉴定成功后,于孕14 d进行标本取材.采用免疫组化方法检测母鼠及胎鼠组织中LCHAD蛋白的分布和表达状况.结果 孕14 d实验组平均动脉压(115.6±3.3)mmHg(1 mmHg =0.133 kPa)和尿蛋白(97.0±4.3)μg·g体质量-1·24h-1较对照组平均动脉压(96.2±4.9)mmHg和尿蛋白(60.8 ±4.9) μg·g体质量-1·24 h-1显著升高(P＜0.05).在两组动物中均可见到母鼠肝、肾、心、胎盘组织中LCHAD蛋白呈阳性表达,实验组母体肝、胎盘LCHAD的IA值强度较对照组明显降低(P＜0.05).胎鼠的肝、肾、心、肺和神经视网膜组织中,两组均有LCHAD表达,但IA值差异无统计学意义(P＞0.05).结论 妊娠中期LCHAD蛋白在母鼠和胎鼠重要器官均广泛表达分布,在妊娠期LCHAD与母体能量代谢、胎盘的发育及胎儿生长发育有关;子痫前期样改变时母体肝脏和胎盘的LCHAD表达存在异常.
目的 探討長鏈脂肪痠氧化酶LCHAD在妊娠中期子癇前期樣改變母鼠和胎鼠組織中的蛋白錶達及分佈.方法 將C57BL/6J孕鼠于孕1id皮下註射一氧化氮閤酶抑製劑建立子癇前期樣模型,同時設立註射生理鹽水的正常妊娠對照組,模型鑒定成功後,于孕14 d進行標本取材.採用免疫組化方法檢測母鼠及胎鼠組織中LCHAD蛋白的分佈和錶達狀況.結果 孕14 d實驗組平均動脈壓(115.6±3.3)mmHg(1 mmHg =0.133 kPa)和尿蛋白(97.0±4.3)μg·g體質量-1·24h-1較對照組平均動脈壓(96.2±4.9)mmHg和尿蛋白(60.8 ±4.9) μg·g體質量-1·24 h-1顯著升高(P＜0.05).在兩組動物中均可見到母鼠肝、腎、心、胎盤組織中LCHAD蛋白呈暘性錶達,實驗組母體肝、胎盤LCHAD的IA值彊度較對照組明顯降低(P＜0.05).胎鼠的肝、腎、心、肺和神經視網膜組織中,兩組均有LCHAD錶達,但IA值差異無統計學意義(P＞0.05).結論 妊娠中期LCHAD蛋白在母鼠和胎鼠重要器官均廣汎錶達分佈,在妊娠期LCHAD與母體能量代謝、胎盤的髮育及胎兒生長髮育有關;子癇前期樣改變時母體肝髒和胎盤的LCHAD錶達存在異常.
목적 탐토장련지방산양화매LCHAD재임신중기자간전기양개변모서화태서조직중적단백표체급분포.방법 장C57BL/6J잉서우잉1id피하주사일양화담합매억제제건립자간전기양모형,동시설립주사생리염수적정상임신대조조,모형감정성공후,우잉14 d진행표본취재.채용면역조화방법검측모서급태서조직중LCHAD단백적분포화표체상황.결과 잉14 d실험조평균동맥압(115.6±3.3)mmHg(1 mmHg =0.133 kPa)화뇨단백(97.0±4.3)μg·g체질량-1·24h-1교대조조평균동맥압(96.2±4.9)mmHg화뇨단백(60.8 ±4.9) μg·g체질량-1·24 h-1현저승고(P＜0.05).재량조동물중균가견도모서간、신、심、태반조직중LCHAD단백정양성표체,실험조모체간、태반LCHAD적IA치강도교대조조명현강저(P＜0.05).태서적간、신、심、폐화신경시망막조직중,량조균유LCHAD표체,단IA치차이무통계학의의(P＞0.05).결론 임신중기LCHAD단백재모서화태서중요기관균엄범표체분포,재임신기LCHAD여모체능량대사、태반적발육급태인생장발육유관;자간전기양개변시모체간장화태반적LCHAD표체존재이상.
Objective To explore the protein expression and distribution of long-chain 3-hydroxyacyl-CoA dehydrogenase (LCHAD) in various maternal and fetal tissues in preeclampsia-like and normal pregnant mouse models during the middle trimester.Methods C57BL/6J mice were divided into preeclampsia-like experimental group with Nw-nitro-L-arginine-methyl ester injection and normal pregnancy control group with normal saline injection.Samples were taken at Day 14 of gestation.Immunohistochemistry was used to detect the LCHAD protein expression and distribution in various maternal and fetal tissues.Results The mean arterial pressure was (115.6 ± 3.3) mmHg (1 mmHg =0.133 kPa) in experimental group and (96.2 ± 4.9) mmHg in control group (P ＜ 0.05).The urine protein contents were (97.0 ± 4.3) μg/g body weight/24h in experimental group and (60.8 ± 4.9) μg/g body weight/24h in control group (P ＜ 0.05).In both groups,LCHAD protein was expressed in maternal liver,kidney,heart and placenta tissues during the middle trimester.And maternal hepatic tissue,myocardial tissue,renal tubule and spongiotrophoblast showed a strong positive expression,placental labyrinth layer a moderate positive expression and glomerulus a weakly positive expression.LCHAD expression of maternal liver and placenta decreased significantly in experimental group (P ＜0.05).At Day 14 of pregnancy,LCHAD was expressed in fetal liver,kidney,heart,lung tissue and neural retina.And renal tubule,pulmonary epithelial tissue and neural retina showed a strong positive expression,fetal liver a moderate positive expression and myocardial tissue and glomerulus a weakly positive expression.There was no significant inter-group difference in fetal tissues.Conclusion LCHAD is expressed and distributed widely in maternal and fetal tissues during the middle trimester.And it may play an important role in maternal metabolism and placentafetus development during pregnancy.There is an abnormal protein expression LCHAD in maternal liver and placenta with preeclampsia-like changes.
