中华眼底病杂志
中華眼底病雜誌
중화안저병잡지
CHINESE JOURNAL OF OCULAR FUNDUS DISEASES
2015年
1期
41-44
,共4页
王朱健%周旻%吉建%沈健%汤荔%曹文俊%徐格致
王硃健%週旻%吉建%瀋健%湯荔%曹文俊%徐格緻
왕주건%주민%길건%침건%탕려%조문준%서격치
弓蛔虫病/诊断%幼虫移行症%弓蛔线虫病%免疫球蛋白G/诊断应用
弓蛔蟲病/診斷%幼蟲移行癥%弓蛔線蟲病%免疫毬蛋白G/診斷應用
궁회충병/진단%유충이행증%궁회선충병%면역구단백G/진단응용
Toxocariasis/diagnosis%Larva migrans%Toxascariasis%Immunoglobulin G/diagnostic use
目的 评价眼内液犬弓蛔虫特异性IgG抗体检测在眼弓蛔虫病诊断中的作用.方法 临床确诊为眼弓蛔虫病的50例患者(眼弓蛔虫病组)、就诊于耳鼻喉科的152例患者(血清对照组)及就诊于眼科的70例其他病患者(眼内液对照组)纳入研究.眼弓蛔虫病组收集房水样本28份,玻璃体液样本22份,血清样本50份;血清对照组收集血清样本152份;眼内液对照组收集房水样本46份,玻璃体液样本24份.采用酶联免疫吸附试验检测所有受检者眼内液及血清中犬弓蛔虫特异性IgG抗体的阳性率.同时对眼弓蛔虫病组患者的血清及眼内液样本进行配对检测,对比分析两者之间的犬弓蛔虫特异性IgG水平的差异.结果 眼弓蛔虫病组血清犬弓蛔虫特异性IgG阳性率为68.00％,U值为20.42±17.01;眼内液犬弓蛔虫特异性IgG阳性率为88.00％,U值为25.72±23.04.血清对照组血清犬弓蛔虫特异性IgG阳性率为2.63％,U值为2.37±2.71;眼弓蛔虫病组患者血清特异性IgG水平较血清对照组明显偏高,差异有统计学意义(Z=8.962,P=0.000).眼内液对照组未见眼内液犬弓蛔虫特异性IgG阳性者,U值为0.69±0.34;眼弓蛔虫病组患者眼内液特异性IgG水平较眼内液对照组明显偏高,差异有统计学意义(Z=8.120,P=0.000).眼弓蛔虫病组50例患者中,血清及眼内液犬弓蛔虫特异性IgG均为阳性者28例,占56.00％;血清犬弓蛔虫特异性IgG阳性,而眼内液犬弓蛔虫特异性IgG阴性者6例,占12.00％;血清犬弓蛔虫特异性IgG阴性,而眼内液犬弓蛔虫特异性IgG阳性者16例,占32.00％.血清与眼内液犬弓蛔虫特异性IgG阳性率比较,差异有统计学意义(x2=4.720,P=0.028).眼弓蛔虫病组患者的血清及眼内液样本配对检测结果显示,32例患者的眼内液犬弓蛔虫特异性IgG水平高于血清样本,占64.00％.结论 眼内液犬弓蛔虫特异性IgG抗体检测相对于血清具有更高的检出率.眼内液与血清犬弓蛔虫特异性IgG抗体的共同检测可能具有更好的诊断价值.
目的 評價眼內液犬弓蛔蟲特異性IgG抗體檢測在眼弓蛔蟲病診斷中的作用.方法 臨床確診為眼弓蛔蟲病的50例患者(眼弓蛔蟲病組)、就診于耳鼻喉科的152例患者(血清對照組)及就診于眼科的70例其他病患者(眼內液對照組)納入研究.眼弓蛔蟲病組收集房水樣本28份,玻璃體液樣本22份,血清樣本50份;血清對照組收集血清樣本152份;眼內液對照組收集房水樣本46份,玻璃體液樣本24份.採用酶聯免疫吸附試驗檢測所有受檢者眼內液及血清中犬弓蛔蟲特異性IgG抗體的暘性率.同時對眼弓蛔蟲病組患者的血清及眼內液樣本進行配對檢測,對比分析兩者之間的犬弓蛔蟲特異性IgG水平的差異.結果 眼弓蛔蟲病組血清犬弓蛔蟲特異性IgG暘性率為68.00％,U值為20.42±17.01;眼內液犬弓蛔蟲特異性IgG暘性率為88.00％,U值為25.72±23.04.血清對照組血清犬弓蛔蟲特異性IgG暘性率為2.63％,U值為2.37±2.71;眼弓蛔蟲病組患者血清特異性IgG水平較血清對照組明顯偏高,差異有統計學意義(Z=8.962,P=0.000).眼內液對照組未見眼內液犬弓蛔蟲特異性IgG暘性者,U值為0.69±0.34;眼弓蛔蟲病組患者眼內液特異性IgG水平較眼內液對照組明顯偏高,差異有統計學意義(Z=8.120,P=0.000).眼弓蛔蟲病組50例患者中,血清及眼內液犬弓蛔蟲特異性IgG均為暘性者28例,佔56.00％;血清犬弓蛔蟲特異性IgG暘性,而眼內液犬弓蛔蟲特異性IgG陰性者6例,佔12.00％;血清犬弓蛔蟲特異性IgG陰性,而眼內液犬弓蛔蟲特異性IgG暘性者16例,佔32.00％.血清與眼內液犬弓蛔蟲特異性IgG暘性率比較,差異有統計學意義(x2=4.720,P=0.028).眼弓蛔蟲病組患者的血清及眼內液樣本配對檢測結果顯示,32例患者的眼內液犬弓蛔蟲特異性IgG水平高于血清樣本,佔64.00％.結論 眼內液犬弓蛔蟲特異性IgG抗體檢測相對于血清具有更高的檢齣率.眼內液與血清犬弓蛔蟲特異性IgG抗體的共同檢測可能具有更好的診斷價值.
목적 평개안내액견궁회충특이성IgG항체검측재안궁회충병진단중적작용.방법 림상학진위안궁회충병적50례환자(안궁회충병조)、취진우이비후과적152례환자(혈청대조조)급취진우안과적70례기타병환자(안내액대조조)납입연구.안궁회충병조수집방수양본28빈,파리체액양본22빈,혈청양본50빈;혈청대조조수집혈청양본152빈;안내액대조조수집방수양본46빈,파리체액양본24빈.채용매련면역흡부시험검측소유수검자안내액급혈청중견궁회충특이성IgG항체적양성솔.동시대안궁회충병조환자적혈청급안내액양본진행배대검측,대비분석량자지간적견궁회충특이성IgG수평적차이.결과 안궁회충병조혈청견궁회충특이성IgG양성솔위68.00％,U치위20.42±17.01;안내액견궁회충특이성IgG양성솔위88.00％,U치위25.72±23.04.혈청대조조혈청견궁회충특이성IgG양성솔위2.63％,U치위2.37±2.71;안궁회충병조환자혈청특이성IgG수평교혈청대조조명현편고,차이유통계학의의(Z=8.962,P=0.000).안내액대조조미견안내액견궁회충특이성IgG양성자,U치위0.69±0.34;안궁회충병조환자안내액특이성IgG수평교안내액대조조명현편고,차이유통계학의의(Z=8.120,P=0.000).안궁회충병조50례환자중,혈청급안내액견궁회충특이성IgG균위양성자28례,점56.00％;혈청견궁회충특이성IgG양성,이안내액견궁회충특이성IgG음성자6례,점12.00％;혈청견궁회충특이성IgG음성,이안내액견궁회충특이성IgG양성자16례,점32.00％.혈청여안내액견궁회충특이성IgG양성솔비교,차이유통계학의의(x2=4.720,P=0.028).안궁회충병조환자적혈청급안내액양본배대검측결과현시,32례환자적안내액견궁회충특이성IgG수평고우혈청양본,점64.00％.결론 안내액견궁회충특이성IgG항체검측상대우혈청구유경고적검출솔.안내액여혈청견궁회충특이성IgG항체적공동검측가능구유경호적진단개치.
Objective To evaluate the clinical value of detecting specific IgG against Toxocara canis in intraocular fluid for the diagnosis of ocular toxocariasis(OT).Methods Fifty patients diagnosed as having OT(OT group),152 otolaryngology patients (serum control group) and 70 other oculopathy patients (intraocular fluid control group) were enrolled in the study.Intraocular fluids(28 aqueous humor and 22 vitreous humor) and serum samples of OT group were analyzed for specific IgG against Toxocara canis by enzymelinked immunosorbent assay,so were the intraocular fluids (46 aqueous humor and 24 vitreous humor) and 152 serum samples of two control group.Specific IgG level was compared between paired serum and intraocular fluids of OT group.Results 68.00％ serum samples of OT group were positive for specific IgG against Toxocara canis and the U value was 20.42±17.01.The positive rate was 88.00％ and U value 25.72±23.04 in intraocular fluids.In serum control group,it was 2.63％ and 2.37±2.71 respectively.The intraocular fluids were negative and U value 0.69 ± 0.34 in intraocular fluid control group.The difference of specific IgG level was proved significant between OT group and control group in both serum and intraocular fluid (Z=8.962,8.120; P=0.000,0.000).Twenty-eight patients (56.00％) were positive for specific IgG in both serum and intraocular fluid.Six patients (12.00％) were positive only in serum,and 16 patients (32.00％) only in intraocular fluid.The positive rate was significantly higher in intraocular fluid than in serum (x2 =4.720,P=0.028).Moreover,64.00％ intraocular fluid showed higher specific IgG level than pared serum.Conclusion The complementary detection of intraocular specific IgG have referential value in diagnosing ocular toxocariasis.
