CAJ | 학술논문

目的研究血管紧张素转换酶基因2350G→A单核苷酸多态性(ACE2350G/A)及胃促胰酶(Chymase)基因多态性在高血压伴左心室肥厚(LVH)人群中心房颤动(房颤)及非房颤患者中的分布,探讨高血压伴LVH患者房颤发生的分子遗传学机制,为房颤的防治提供临床和实验依据.方法 2010年8月至2013年6月泰州市人民医院收治的408例高血压伴LVH住院患者,根据有无房颤分为LVH-房颤组和LVH组,利用聚合酶链反应(PCR)及限制性酶切技术进行检测ACE2350G/A及Chymase基因的CMA/B多态性.结果 LVH-房颤组ACE2350基因G、A等位基因的频率明显高于LVH组(x2=5.503,P=0.019).ACE2350基因多态性与高血压伴LVH患者房颤相关.CMA/B基因在房颤组与LVH组G、A等位基因频率差异无统计学意义(x2=0.933,P=0.334),CMA/B基因与高血压伴LVH患者房颤发生无显著相关.与正常人相比,各类型LVH患者的CMA/B基因的GG,AA及G、A等位基因频率差异均有统计学意义.结论 ACE2350基因多态性与高血压伴LVH患者房颤发生相关,AA基因型增加房颤的发生风险,A等位基因为房颤发生的危险基因.CMA/B基因与高血压伴LVH患者房颤发生无显著相关.
목적 연구혈관긴장소전환매기인2350G→A단핵감산다태성(ACE2350G/A)급위촉이매(Chymase)기인다태성재고혈압반좌심실비후(LVH)인군중심방전동(방전)급비방전환자중적분포,탐토고혈압반LVH환자방전발생적분자유전학궤제,위방전적방치제공림상화실험의거.방법 2010년8월지2013년6월태주시인민의원수치적408례고혈압반LVH주원환자,근거유무방전분위LVH-방전조화LVH조,이용취합매련반응(PCR)급한제성매절기술진행검측ACE2350G/A급Chymase기인적CMA/B다태성.결과 LVH-방전조ACE2350기인G、A등위기인적빈솔명현고우LVH조(x2=5.503,P=0.019).ACE2350기인다태성여고혈압반LVH환자방전상관.CMA/B기인재방전조여LVH조G、A등위기인빈솔차이무통계학의의(x2=0.933,P=0.334),CMA/B기인여고혈압반LVH환자방전발생무현저상관.여정상인상비,각류형LVH환자적CMA/B기인적GG,AA급G、A등위기인빈솔차이균유통계학의의.결론 ACE2350기인다태성여고혈압반LVH환자방전발생상관,AA기인형증가방전적발생풍험,A등위기인위방전발생적위험기인.CMA/B기인여고혈압반LVH환자방전발생무현저상관.
Objective The present study was focused on the ACE2350G/A gene polymorphism and Chymase gene polymorphism and distribution in hypertension patients with left ventricular hypertrophy(LVH).Methods The samples were collected in Taizhou People's hospital form August 2010 to June 2013,which included 408 cases of hospitalized hypertension patients with LVH,and were divided into LVH group and LVH with AF group.The polymerase chain reaction(PCR) and restriction enzyme digestion technique were used to investigate the CMA/B gene polymorphism of ACE2350G/A and Chymase gene polymorphism.The data were analyzed by multivariate Logistic regression,and the statistical analysis was performed by SPSS software version 18.0.Results LVH-AF group exhibited a higher G and A allele frequency than that in LVH group in the patients' ACE2350 genes(x2 =5.503,P=0.019).There was no significant statistically difference between G and A allele frequency of CMA/B gene in AF group and LVH group (x2 =0.933,P =0.334).Conclusion The polymorphisms of ACE2350 gene had a close relationship with the happening of AF in hypertension patients with left ventricular hypertrophy.The expression of AA genotype increases the risk of AF,and the A gene alleles can be regarded as a high risk genetic factor.CMA/B gene had nothing to do with the occurrence of AF in hypertension patients with LVH.