中华消化杂志
中華消化雜誌
중화소화잡지
Chinese Journal of Digestion
2014年
12期
826-830
,共5页
吴萍%罗斌%李莹杰%周璐%易丽莎%许树长
吳萍%囉斌%李瑩傑%週璐%易麗莎%許樹長
오평%라빈%리형걸%주로%역려사%허수장
食管炎,消化性%二硫代氨基吡咯烷%NF-κB%白细胞介素-6
食管炎,消化性%二硫代氨基吡咯烷%NF-κB%白細胞介素-6
식관염,소화성%이류대안기필각완%NF-κB%백세포개소-6
Esophagitis,peptic%PDTC%NF-kappa B%Interleukin-6
目的 观察NF-κB抑制剂二硫代氨基吡咯烷(PDTC)对慢性混合反流性食管炎大鼠食管黏膜的保护作用及对NFκB/IL-6信号通路的影响.方法 40只健康清洁级SD雄性大鼠平均分为健康对照组、假手术组、模型对照组、奥美拉唑组、PDTC组,每组8只.除健康对照组和假手术组以外的大鼠建立慢性混合反流性食管炎大鼠模型.健康对照组、假手术组及模型对照组大鼠予以0.9％ NaCl溶液2 mL/d腹腔注射,奥美拉唑组大鼠每天按体质量予以20 mg/kg奥美拉唑腹腔注射,PDTC组大鼠每天按体质量予以100 mg/kg PDTC腹腔注射,6周后处死,肉眼及光学显微镜下观察食管组织的形态学改变并评分,应用ELISA法检测血清NF-κB p65、IL-6蛋白在各组大鼠中的表达.各组间均数比较采用t检验.结果 健康对照组、假手术组、模型对照组、奥美拉唑组、PDTC组5组大鼠的食管黏膜肉眼评分分别为0.000±0.000、0.000±0.000、2.250±0.707、1.125±0.835、1.429±0.535,病理评分分别为0.000±0.000、0.000±0.000、2.625±0.518、1.500±0.535、1.429±0.535,与模型对照组比较,健康对照组、假手术组、奥美拉唑组及PDTC组大鼠食管黏膜的肉眼、病理积分均降低,差异均有统计学意义(t肉眼 =7.603、7.603、2.909、2.506,t病理评分=9.674、9.674、4.277、4.399;P均＜0.05).健康对照组、假手术组、奥美拉唑组、PDTC组大鼠的血清NF-κB p65蛋白含量分别为(68.618±18.450) pg/mL、(77.824±22.228) pg/mL、(106.693±45.312) pg/mL、(103.781±42.502) pg/mL,与模型对照组[(184.882±49.165) pg/mL]比较明显降低,差异均有统计学意义(t=6.262、5.612、3.308、3.427,P均＜0.05);血清IL-6蛋白含量分别为(24.826±4.008) pg/mL、(23.599±4.351) pg/mL、(32.370±11.657) pg/mL、(33.694±10.394) pg/mL,与模型对照组[(51.378±9.697) pg/mL]比较亦显著降低,差异均有统计学意义(t=7.157、7.393、3.546、3.392,P均＜0.05).PDTC组大鼠食管黏膜的肉眼、病理积分及血清NF-κB p65、IL-6蛋白含量与奥美拉唑组相比无明显升高,差异均无统计学意义(P均＞0.05).结论 NF-κB抑制剂PDTC能减轻反流性食管炎大鼠食管黏膜的损伤程度,其机制可能与下调NF-κB/IL6信号通路有关.
目的 觀察NF-κB抑製劑二硫代氨基吡咯烷(PDTC)對慢性混閤反流性食管炎大鼠食管黏膜的保護作用及對NFκB/IL-6信號通路的影響.方法 40隻健康清潔級SD雄性大鼠平均分為健康對照組、假手術組、模型對照組、奧美拉唑組、PDTC組,每組8隻.除健康對照組和假手術組以外的大鼠建立慢性混閤反流性食管炎大鼠模型.健康對照組、假手術組及模型對照組大鼠予以0.9％ NaCl溶液2 mL/d腹腔註射,奧美拉唑組大鼠每天按體質量予以20 mg/kg奧美拉唑腹腔註射,PDTC組大鼠每天按體質量予以100 mg/kg PDTC腹腔註射,6週後處死,肉眼及光學顯微鏡下觀察食管組織的形態學改變併評分,應用ELISA法檢測血清NF-κB p65、IL-6蛋白在各組大鼠中的錶達.各組間均數比較採用t檢驗.結果 健康對照組、假手術組、模型對照組、奧美拉唑組、PDTC組5組大鼠的食管黏膜肉眼評分分彆為0.000±0.000、0.000±0.000、2.250±0.707、1.125±0.835、1.429±0.535,病理評分分彆為0.000±0.000、0.000±0.000、2.625±0.518、1.500±0.535、1.429±0.535,與模型對照組比較,健康對照組、假手術組、奧美拉唑組及PDTC組大鼠食管黏膜的肉眼、病理積分均降低,差異均有統計學意義(t肉眼 =7.603、7.603、2.909、2.506,t病理評分=9.674、9.674、4.277、4.399;P均＜0.05).健康對照組、假手術組、奧美拉唑組、PDTC組大鼠的血清NF-κB p65蛋白含量分彆為(68.618±18.450) pg/mL、(77.824±22.228) pg/mL、(106.693±45.312) pg/mL、(103.781±42.502) pg/mL,與模型對照組[(184.882±49.165) pg/mL]比較明顯降低,差異均有統計學意義(t=6.262、5.612、3.308、3.427,P均＜0.05);血清IL-6蛋白含量分彆為(24.826±4.008) pg/mL、(23.599±4.351) pg/mL、(32.370±11.657) pg/mL、(33.694±10.394) pg/mL,與模型對照組[(51.378±9.697) pg/mL]比較亦顯著降低,差異均有統計學意義(t=7.157、7.393、3.546、3.392,P均＜0.05).PDTC組大鼠食管黏膜的肉眼、病理積分及血清NF-κB p65、IL-6蛋白含量與奧美拉唑組相比無明顯升高,差異均無統計學意義(P均＞0.05).結論 NF-κB抑製劑PDTC能減輕反流性食管炎大鼠食管黏膜的損傷程度,其機製可能與下調NF-κB/IL6信號通路有關.
목적 관찰NF-κB억제제이류대안기필각완(PDTC)대만성혼합반류성식관염대서식관점막적보호작용급대NFκB/IL-6신호통로적영향.방법 40지건강청길급SD웅성대서평균분위건강대조조、가수술조、모형대조조、오미랍서조、PDTC조,매조8지.제건강대조조화가수술조이외적대서건립만성혼합반류성식관염대서모형.건강대조조、가수술조급모형대조조대서여이0.9％ NaCl용액2 mL/d복강주사,오미랍서조대서매천안체질량여이20 mg/kg오미랍서복강주사,PDTC조대서매천안체질량여이100 mg/kg PDTC복강주사,6주후처사,육안급광학현미경하관찰식관조직적형태학개변병평분,응용ELISA법검측혈청NF-κB p65、IL-6단백재각조대서중적표체.각조간균수비교채용t검험.결과 건강대조조、가수술조、모형대조조、오미랍서조、PDTC조5조대서적식관점막육안평분분별위0.000±0.000、0.000±0.000、2.250±0.707、1.125±0.835、1.429±0.535,병리평분분별위0.000±0.000、0.000±0.000、2.625±0.518、1.500±0.535、1.429±0.535,여모형대조조비교,건강대조조、가수술조、오미랍서조급PDTC조대서식관점막적육안、병리적분균강저,차이균유통계학의의(t육안 =7.603、7.603、2.909、2.506,t병리평분=9.674、9.674、4.277、4.399;P균＜0.05).건강대조조、가수술조、오미랍서조、PDTC조대서적혈청NF-κB p65단백함량분별위(68.618±18.450) pg/mL、(77.824±22.228) pg/mL、(106.693±45.312) pg/mL、(103.781±42.502) pg/mL,여모형대조조[(184.882±49.165) pg/mL]비교명현강저,차이균유통계학의의(t=6.262、5.612、3.308、3.427,P균＜0.05);혈청IL-6단백함량분별위(24.826±4.008) pg/mL、(23.599±4.351) pg/mL、(32.370±11.657) pg/mL、(33.694±10.394) pg/mL,여모형대조조[(51.378±9.697) pg/mL]비교역현저강저,차이균유통계학의의(t=7.157、7.393、3.546、3.392,P균＜0.05).PDTC조대서식관점막적육안、병리적분급혈청NF-κB p65、IL-6단백함량여오미랍서조상비무명현승고,차이균무통계학의의(P균＞0.05).결론 NF-κB억제제PDTC능감경반류성식관염대서식관점막적손상정도,기궤제가능여하조NF-κB/IL6신호통로유관.
Objective To observe the protective effects of nuclear factor (NF) κB inhibitor pyrrolidine dithiocarbamate (PDTC) on chronic mixed reflux esophagitis in rats and its influence on NF-κB/interleukin (IL)-6 signaling pathway.Method A total of 40 healthy male Sprague-Dawley (SD) rats were divided into healthy control group,sham operation group,model control group,omeprazole group and PDTC group with eight rats in each group.Except rats in healthy control group and sham operation group,mixed reflux esophagitis model were established in all the other groups.The rats of healthy control group,sham operation group and model control group were all intraperitoneally injected with 2 mL 0.9％ NaCl,rats of omeprazole group were intraperitoneally injected with omeprazole 20 mg/kg,and rats of PDTC group were intraperitoneally injected with PDTC 100 mg/kg every day.After six weeks,the rats were sacrificed,the morphological changes of esophageal tissues were observed and scored by visual inspection and under light microscope.The serum levels of NF-κB p65 and IL-6 in rats of each group were assessed by enzyme linked immunoassay (ELISA).t test was performed for mean comparison among groups.Results The scores of esophageal mucosa judged by visual inspection of healthy control group,sham operation group,model control group,omeprazole group and PDTC group were 0.000 20.000,0.000±0.000,2.250± 0.707,1.125 ± 0.835 and 1.429± 0.535,respectively.The pathological scores were 0.00020.000,0.000±0.000,2.625±0.518,1.500±0.535,1.429±0.535,respectively.Compared with those of model control group,the scores judged by visual inspection and the pathological scores of healthy control group,sham operation group,omeprazole group and PDTC group were lower,and the differences were statistically significant (t=7.603,7.603,2.909,2.506; t=9.674,9.674,4.277,4.399,all P＜0.05).The serum levels of NF-κB p65 protein of healthy control group,sham operation group,omeprazole group and PDTC group were (68.618±18.450) pg/mL,(77.824±22.228) pg/mL,(106.693±45.312) pg/mL and (103.781± 42.502)pg/mL,respectively; compared with that of model group ((184.882±49.165) pg/mL),which were significantly lower and the differences were statistically significant (t=6.262,5.612,3.308 and 3.427,all P＜0.05).The serum levels of IL-6 protein were (24.826±4.008) pg/mL,(23.599±4.351) pg/mL,(32.370± 11.657) pg/mL and (33.694±10.394) pg/mL,respectively,which significantly decreased when compared with that of model group ((51.378±9.697) pg/mL,t=7.157,7.393,3.546 and 3.392,all P＜0.05).There was no significant difference between PDTC group and omeprazole group in the score judged by visual inspection,pathological scores,the serum levels of NF-κB p65 and IL-6 protein (all P＞0.05).Conclusion NF-κB inhibitor PDTC could reduce the injury severity of esophageal mucosal in reflux esophagitis rat,which mechanism might be related with the down-regulation of NF-κB/1L-6 signaling pathway.
