中华消化杂志
中華消化雜誌
중화소화잡지
Chinese Journal of Digestion
2015年
1期
34-38
,共5页
刘姗%陈洪潭%陈李华%徐根云%许国强
劉姍%陳洪潭%陳李華%徐根雲%許國彊
류산%진홍담%진리화%서근운%허국강
胆囊胆固醇结石症%致石饲料%微囊蛋白-1%清道夫受体B族1
膽囊膽固醇結石癥%緻石飼料%微囊蛋白-1%清道伕受體B族1
담낭담고순결석증%치석사료%미낭단백-1%청도부수체B족1
Cholesterol gallstone disease%Lithogenic diet%Caveolin-1%Scavenger receptor class B member 1
目的 探讨微囊蛋白-1在致石饲料诱导的小鼠胆囊胆固醇结石症形成中的作用及其可能的机制.方法 以结石易感C57BL/6小鼠为研究对象,分别给予对照组共6只和实验组共6只小鼠普通饲料和致石饲料饲养4周,检测小鼠胆囊胆固醇结石形成状况、血脂、胆汁脂质含量变化.采用实时定量PCR法和Western蛋白印迹法检测小鼠肝脏及胆囊组织中微囊蛋白-1、清道夫受体B族Ⅰ型(SR-BI)的mRNA和蛋白表达水平和变化.采用t检验比较两组均数.结果 致石饲料饲养后4周,实验组小鼠胆囊成石率为100％,血脂水平明显升高;胆汁中胆固醇成分明显增加,胆盐减少.与对照组相比,微囊蛋白1在实验组小鼠肝脏和胆囊组织中mRNA和蛋白水平表达均显著下调(肝脏mRNA相对定量值为0.53±0.13比1.00±0.32,t=3.330;蛋白相对定量值为0.39±0.07比0.92±0.06,t=10.280;胆囊mRNA相对定量值为0.40±0.22比1.00±0.22,t=3.823;蛋白相对定量值为1.04±0.07比1.34±0.04,t=6.367,P均＜0.01).实验组小鼠肝胆组织的SR-BⅠ蛋白和mRNA相对定量与对照组相比差异均无统计学意义(P均＞0.05).结论 肝胆组织中微囊蛋白-1的mRNA和蛋白表达水平变化可能影响实验小鼠肝胆组织中的脂质代谢和胆固醇转运,在胆囊胆固醇结石的形成中发挥着作用.
目的 探討微囊蛋白-1在緻石飼料誘導的小鼠膽囊膽固醇結石癥形成中的作用及其可能的機製.方法 以結石易感C57BL/6小鼠為研究對象,分彆給予對照組共6隻和實驗組共6隻小鼠普通飼料和緻石飼料飼養4週,檢測小鼠膽囊膽固醇結石形成狀況、血脂、膽汁脂質含量變化.採用實時定量PCR法和Western蛋白印跡法檢測小鼠肝髒及膽囊組織中微囊蛋白-1、清道伕受體B族Ⅰ型(SR-BI)的mRNA和蛋白錶達水平和變化.採用t檢驗比較兩組均數.結果 緻石飼料飼養後4週,實驗組小鼠膽囊成石率為100％,血脂水平明顯升高;膽汁中膽固醇成分明顯增加,膽鹽減少.與對照組相比,微囊蛋白1在實驗組小鼠肝髒和膽囊組織中mRNA和蛋白水平錶達均顯著下調(肝髒mRNA相對定量值為0.53±0.13比1.00±0.32,t=3.330;蛋白相對定量值為0.39±0.07比0.92±0.06,t=10.280;膽囊mRNA相對定量值為0.40±0.22比1.00±0.22,t=3.823;蛋白相對定量值為1.04±0.07比1.34±0.04,t=6.367,P均＜0.01).實驗組小鼠肝膽組織的SR-BⅠ蛋白和mRNA相對定量與對照組相比差異均無統計學意義(P均＞0.05).結論 肝膽組織中微囊蛋白-1的mRNA和蛋白錶達水平變化可能影響實驗小鼠肝膽組織中的脂質代謝和膽固醇轉運,在膽囊膽固醇結石的形成中髮揮著作用.
목적 탐토미낭단백-1재치석사료유도적소서담낭담고순결석증형성중적작용급기가능적궤제.방법 이결석역감C57BL/6소서위연구대상,분별급여대조조공6지화실험조공6지소서보통사료화치석사료사양4주,검측소서담낭담고순결석형성상황、혈지、담즙지질함량변화.채용실시정량PCR법화Western단백인적법검측소서간장급담낭조직중미낭단백-1、청도부수체B족Ⅰ형(SR-BI)적mRNA화단백표체수평화변화.채용t검험비교량조균수.결과 치석사료사양후4주,실험조소서담낭성석솔위100％,혈지수평명현승고;담즙중담고순성분명현증가,담염감소.여대조조상비,미낭단백1재실험조소서간장화담낭조직중mRNA화단백수평표체균현저하조(간장mRNA상대정량치위0.53±0.13비1.00±0.32,t=3.330;단백상대정량치위0.39±0.07비0.92±0.06,t=10.280;담낭mRNA상대정량치위0.40±0.22비1.00±0.22,t=3.823;단백상대정량치위1.04±0.07비1.34±0.04,t=6.367,P균＜0.01).실험조소서간담조직적SR-BⅠ단백화mRNA상대정량여대조조상비차이균무통계학의의(P균＞0.05).결론 간담조직중미낭단백-1적mRNA화단백표체수평변화가능영향실험소서간담조직중적지질대사화담고순전운,재담낭담고순결석적형성중발휘착작용.
Objective To investigate the role and possible mechanism of caveolin-1 (CAV1) in the forming of cholesterol gallstone in mice fed with lithogenic diet.Methods Cholesterol gallstone susceptible C57BL/6 mice were study objects.The mice of control group (n=6) and experiment group (n=6) were fed with normal diet and lithogenic diet for four weeks respectively.The condition of cholesterol gallstone forming,changes of serum lipid and bile composition were measured,and the expressions of CAV1 and scavenger receptor classB member Ⅰ (SR-BⅠ) at mRNA and protein level in the liver and gallbladder were detected by realtime-polymerase chain reaction and Western blot,respectively.The t test was performed for mean comparsion between the two groups.Results The incidence rate of gallstone in experimental group was 100％ after fed with lithogenic diet for four weeks,the lipid level significantly increased,and the proportion of cholesterol in bile raised and bile salt decreased.Compared with those of control group,the expressions of CAV1 at mRNA and protein level in the liver and gallbladder tissues siginificantly decreased (in liver tissue,mRNA 0.53 ± 0.13 vs 1.00 ± 0.32,t =3.330,protein level 0.39 ± 0.07vs 0.92±0.06,t=10.280; in gallbladder tissue,mRNA 0.40±0.22 vs 1.00±0.22,t=3.823,protein level 1.04±0.07 vs 1.34 ± 0.04,t =6.367,all P＜0.01).There was no significant difference in the relative expression of SR-BⅠ at mRNA and protein level in the liver and gallbladder tissues between the mice of experiment group and control group.Conclusion The changes of CAV 1 expression at mRNA and protein level in liver and gallbladder tissues may affect lipids metabolism and cholesterol transportation in liver and gallbladder tissues of experiment mice,which might play an important role in the formation of cholesterol gallstone.
