中华外科杂志
中華外科雜誌
중화외과잡지
CHINESE JOURNAL OF SURGERY
2015年
2期
116-120
,共5页
椎问盘移位%多态性,限制性片段长度%蛋白聚糖类
椎問盤移位%多態性,限製性片段長度%蛋白聚糖類
추문반이위%다태성,한제성편단장도%단백취당류
Intervertebral disk displacement%Polymorphism,restriction fragment length%Proteoglycans
目的 探讨人类聚集蛋白聚糖(Aggrecan)基因的表达及Aggrecan基因串联重复多态性(VNTR)与腰椎问盘突出症(LDH)的相关性.方法 收集2004年1月至2014年1月在中国医科大学附属第一医院骨科接受手术治疗且符合纳入和排除标准的89例患者及1 13名健康人的临床资料.病变组为患有LDH的患者74例,对照组为未患有LDH的脊柱创伤患者15例及健康人113名.收集每例手术患者切除的椎间盘和血液标本及健康人的血液标本.应用Western blot法检测两组患者椎间盘中Aggrecan蛋白的表达情况;应用RT-PCR技术检测两组患者血液中Aggrecan VNTR多态性分布情况.检测结果的比较采用x2检验.结果 Western blot检测结果显示,对照组椎间盘中Aggrecan蛋白的相对表达量高于病变组(对照组Aggrecan表达阳性率为86.67％,病例组Aggrecan表达阳性率为13.51％;x2=34.83,P＜0.05).RT-PCR检测结果显示,病变组中等位基因25及等位基因21的基因频率高于对照组(A25病变组=22.97％,A25对照组=12.11％,x2A25=8.20,PA25=0.004;A21病变组=6.76％,A21对照组=0.39％,x2A21=14.35,PA21=0.000).病变组不表达Aggrecan人群中有1个或2个≤等位基因25的个体,多于有2个＞等位基因25等位基因的个体(x2=5.69,P =0.017);对照组二者分布无差异(x2 =0.29,P =0.590).结论 LDH患者中Aggrecan等位基因25及21串联重复序列片段出现频率较高,Aggrecan与LDH有相关性;LDH患者中Aggrecan VNTR与Aggrecan的表达有相关性.
目的 探討人類聚集蛋白聚糖(Aggrecan)基因的錶達及Aggrecan基因串聯重複多態性(VNTR)與腰椎問盤突齣癥(LDH)的相關性.方法 收集2004年1月至2014年1月在中國醫科大學附屬第一醫院骨科接受手術治療且符閤納入和排除標準的89例患者及1 13名健康人的臨床資料.病變組為患有LDH的患者74例,對照組為未患有LDH的脊柱創傷患者15例及健康人113名.收集每例手術患者切除的椎間盤和血液標本及健康人的血液標本.應用Western blot法檢測兩組患者椎間盤中Aggrecan蛋白的錶達情況;應用RT-PCR技術檢測兩組患者血液中Aggrecan VNTR多態性分佈情況.檢測結果的比較採用x2檢驗.結果 Western blot檢測結果顯示,對照組椎間盤中Aggrecan蛋白的相對錶達量高于病變組(對照組Aggrecan錶達暘性率為86.67％,病例組Aggrecan錶達暘性率為13.51％;x2=34.83,P＜0.05).RT-PCR檢測結果顯示,病變組中等位基因25及等位基因21的基因頻率高于對照組(A25病變組=22.97％,A25對照組=12.11％,x2A25=8.20,PA25=0.004;A21病變組=6.76％,A21對照組=0.39％,x2A21=14.35,PA21=0.000).病變組不錶達Aggrecan人群中有1箇或2箇≤等位基因25的箇體,多于有2箇＞等位基因25等位基因的箇體(x2=5.69,P =0.017);對照組二者分佈無差異(x2 =0.29,P =0.590).結論 LDH患者中Aggrecan等位基因25及21串聯重複序列片段齣現頻率較高,Aggrecan與LDH有相關性;LDH患者中Aggrecan VNTR與Aggrecan的錶達有相關性.
목적 탐토인류취집단백취당(Aggrecan)기인적표체급Aggrecan기인천련중복다태성(VNTR)여요추문반돌출증(LDH)적상관성.방법 수집2004년1월지2014년1월재중국의과대학부속제일의원골과접수수술치료차부합납입화배제표준적89례환자급1 13명건강인적림상자료.병변조위환유LDH적환자74례,대조조위미환유LDH적척주창상환자15례급건강인113명.수집매례수술환자절제적추간반화혈액표본급건강인적혈액표본.응용Western blot법검측량조환자추간반중Aggrecan단백적표체정황;응용RT-PCR기술검측량조환자혈액중Aggrecan VNTR다태성분포정황.검측결과적비교채용x2검험.결과 Western blot검측결과현시,대조조추간반중Aggrecan단백적상대표체량고우병변조(대조조Aggrecan표체양성솔위86.67％,병례조Aggrecan표체양성솔위13.51％;x2=34.83,P＜0.05).RT-PCR검측결과현시,병변조중등위기인25급등위기인21적기인빈솔고우대조조(A25병변조=22.97％,A25대조조=12.11％,x2A25=8.20,PA25=0.004;A21병변조=6.76％,A21대조조=0.39％,x2A21=14.35,PA21=0.000).병변조불표체Aggrecan인군중유1개혹2개≤등위기인25적개체,다우유2개＞등위기인25등위기인적개체(x2=5.69,P =0.017);대조조이자분포무차이(x2 =0.29,P =0.590).결론 LDH환자중Aggrecan등위기인25급21천련중복서렬편단출현빈솔교고,Aggrecan여LDH유상관성;LDH환자중Aggrecan VNTR여Aggrecan적표체유상관성.
Objective To study the expression of Aggrecan and the relationship between the variable number of tandem repeats (VNTR) of Aggrecan and lumbar disc herniation (LDH).Methods The disease group comprised of 74 patients already diagnosed with symptomatic LDH.The control group consisted of 15 patients restricted to spinal trauma and 113 healthy blood donors without symptoms of LDH who were not diagnosed with LDH.Disc tissue samples were obtained from surgical operations and blood samples were donated from all participants.The Aggrecan expression in isolated tissues was assessed by western blot using specific antibodies.The Aggrecan gene VNTR region was analyzed by PCR.Results The Aggrecan expression positive rate of control group was statistically and significantly higher (control group:86.67％,disease group:13.51％ ; x2 =34.83,P ＜ 0.05) than that of the disease group.Moreover,there was a statistically significant higher frequency of Allele 25 or Allele 21 in disease group compared to controls (A25disease group =22.97％ A25control group =12.11％ x2A25 =8.20,PA25 =0.004; A21disease group=6.76％,A21control group =0.39％,x2A21 =14.35,PA21 =0.000).Compared to the participants with 2 Alleles ＞ 25 repeats,subjects with 1 or 2 Alleles ≤25 repeats statistically and significantly over represented the disease group without the expression of Aggrecan (x2 =5.69,P =0.017).Conclusions The findings suggest a relationship between Aggrecan and symptomatic LDH,where symptomatic LDH has a tendency of allele 21 and allele 25 repeats.In addition,an association between the distribution of Aggrecan gene VNTR polymorphism and the expression of Aggrecan is observed in symptomatic LDH.
