CAJ | 학술논문

目的探讨建立人体膀胱癌细胞系BIU-87膀胱原位移植瘤模型的有效方法,为膀胱癌实验动物的相关研究提供理论基础.方法 BALB-C系裸鼠20只,分成A、B两组(各10只),A组采用手术方法于膀胱壁内注射人体膀胱癌细胞系BIU-87,B组经膀胱灌注人体膀胱癌细胞系BIU-87,分别观察不同阶段两组裸鼠膀胱肿瘤的发生发展情况.实验终点(4周)处死裸鼠,进行瘤体大小及组织学检测.结果 A组裸鼠中,一只于术后第2d死亡,其余存活9只中,8只成瘤,成瘤率为89％;B组10只均未成瘤.结论经膀胱灌注不能建立理想的膀胱原位移植瘤模型,手术方法建立膀胱原位移植瘤模型的效果确切,能较好地模拟人膀胱肿瘤的疾病发展过程,对国人膀胱肿瘤的研究具有一定应用价值.
목적 탐토건립인체방광암세포계BIU-87방광원위이식류모형적유효방법,위방광암실험동물적상관연구제공이론기출.방법 BALB-C계라서20지,분성A、B량조(각10지),A조채용수술방법우방광벽내주사인체방광암세포계BIU-87,B조경방광관주인체방광암세포계BIU-87,분별관찰불동계단량조라서방광종류적발생발전정황.실험종점(4주)처사라서,진행류체대소급조직학검측.결과 A조라서중,일지우술후제2d사망,기여존활9지중,8지성류,성류솔위89％;B조10지균미성류.결론 경방광관주불능건립이상적방광원위이식류모형,수술방법건립방광원위이식류모형적효과학절,능교호지모의인방광종류적질병발전과정,대국인방광종류적연구구유일정응용개치.
Objectives To investigate the effective method of establishing bladder Orthotopic transplantation tumor model with human bladder cancer cell line BIU-87 to provide theoretical basis for the study of experimental animals of bladder cancer.Methods 20 BALB-C nude mice were divided into group A and B (10 PCs),group A were injected with human bladder cancer cell line BIU-87 into the bladder wall through surgical method,B group were instilled with human bladder cancer cell line BIU-87 through bladder,progression of bladder tumor of the two groups was respectively observed in different stage.All mice were executed at experimental endpoint(week 4),tumor size and histological detection were evalued.Results A mouse of group A died on the first day after operation,tumor occured in 8 of the rest surviving mice,tumor formation rate was 89％ ; no tumor occured in B group.Conclusions Ideal orthotopic transplantation tumor model cannot established through bladder Instillation,surgery method to establish Orthotopic transplantation tumor model can definitely simulate development process of human bladder tumor disease better,which enjoy applicational value on the study of Chinese bladder tumors.