CAJ | 학술논문

目的评估液基细胞学(TCT)结合DNA定量分析及免疫组化(Ki67、P16)对宫颈上皮内瘤病变筛查的应用价值.方法对参预宫颈癌前病变筛查的妇女,同时进行液基细胞,DNA定量分析及免筛组化(Ki67、P16)方法学评估及临床应用价值探讨.结果液基细胞学筛查阳性率为11.63％,敏感性与特异性分别为81.28％、27.50％.DNA定量分析筛查阳性率为11.87％,敏感性及特异性分别为:89.83％、19.51％; TCT与DNA联合筛查阳性率为13.54％,敏感性及特异性分别为94.11％、15.36％.免疫组化(Ki67、P16)的阳性表达率分别为84％、56％,两者之间差异有统计学意义(P＜0.05).结论液基细胞学结合DNA定量分析,再辅以免疫组化(Ki67、P16),不但可以提高筛查的阳性率、敏感性、特异性,而且可以了解宫颈癌及癌前病变各阶段的预后与转归,病变程度.更有利于早期诊断及早期治疗.
목적 평고액기세포학(TCT)결합DNA정량분석급면역조화(Ki67、P16)대궁경상피내류병변사사적응용개치.방법 대삼예궁경암전병변사사적부녀,동시진행액기세포,DNA정량분석급면사조화(Ki67、P16)방법학평고급림상응용개치탐토.결과 액기세포학사사양성솔위11.63％,민감성여특이성분별위81.28％、27.50％.DNA정량분석사사양성솔위11.87％,민감성급특이성분별위:89.83％、19.51％; TCT여DNA연합사사양성솔위13.54％,민감성급특이성분별위94.11％、15.36％.면역조화(Ki67、P16)적양성표체솔분별위84％、56％,량자지간차이유통계학의의(P＜0.05).결론 액기세포학결합DNA정량분석,재보이면역조화(Ki67、P16),불단가이제고사사적양성솔、민감성、특이성,이차가이료해궁경암급암전병변각계단적예후여전귀,병변정도.경유리우조기진단급조기치료.
Objective To evaluate the application value of thinprep cytologic test (TCT) combined with DNA quantitative analysis and immunohistochemistry (Ki67,P16) in screening cervical intraepithelial neoplasia.Methods Women participating in cervical premalignant screening received TCT,DNA quantitative analysis and immunohistochemistry (Ki67,P16),then made methodological evaluation and clinical application value discussion.Results The positive rate of TCT screening was 11.63％,sensitivity and specificity were 81.28％ and 27.50％ respectively.The positive rate of DNA quantitative analysis screening was 11.87％,sensitivity and specificity were 89.83％ and 19.51％ respectively.The positive rate of TCT combined with DNA quantitative analysis screening was 13.54％,sensitivity and specificity were 94.11％ and 15.36％ respectively.The positive expression rate of immunohistochemistry (Ki67,P16) were 84％ and 56％respectively,with statistically significant difference (P ＜ 0.05).Conclusion The combination ofTCT,DNA quantitative analysis and immunohistochemistry (Ki67,P 16) can not only improve positive rate,sensitivity,specificity of screening,but also show more about the prognosis and turnover of cervical cancer and each precancerous stage,which is conducive to early diagnosis and early treatment.