国际医药卫生导报
國際醫藥衛生導報
국제의약위생도보
INTERNATIONAL MEDICINE & HEALTH GUIDANCE NEWS
2015年
1期
9-11,15
,共4页
DNA聚合酶β%宫颈上皮内瘤变%宫颈癌
DNA聚閤酶β%宮頸上皮內瘤變%宮頸癌
DNA취합매β%궁경상피내류변%궁경암
pol(B)%CIN%CC
目的 探讨DNA聚合酶β(DNA polymerase beta,polβ)在宫颈上皮内瘤变(cervical intraepithelial neoplasia,CIN)和宫颈癌(cervical carcinoma,CC)中的表达及其临床意义.方法 采用RT-PCR方法检测正常宫颈、CIN、CC组织中polβ mRNA的表达;Western Blot检测这些组织中polβ蛋白相对表达量.结果 正常宫颈、CIN Ⅰ、CINⅡ、CINⅢ、CC组织polβ mRNA分别为(0.23±0.08)、(0.25 ±0.11)、(0.36±0.18)、(0.62±0.22)、(0.85±0.28);正常宫颈、CINⅡ、CINⅢ、CC组间polβ mRNA表达差异有统计学意义(P＜0.05).正常宫颈、CIN Ⅰ、CINⅡ、CINⅢ、CC组织polβ蛋白相对表达量分别为(19±8)％、(18±6)％、(43±17)％、(72±26)％、(93±31)％;正常宫颈、CINⅡ、CINⅢ、CC组间pol β蛋白表达差异有统计学意义(P＜0.05).CIN Ⅰ组织polβ mRNA和蛋白表达与正常宫颈组织比较差异均无统计学意义(P＞0.05).结论 从CINⅡ至CCpolβ表达逐步递增,pol β在CC发生发展中起重要作用,polβ可能成为预测CC进展的生物学指标.
目的 探討DNA聚閤酶β(DNA polymerase beta,polβ)在宮頸上皮內瘤變(cervical intraepithelial neoplasia,CIN)和宮頸癌(cervical carcinoma,CC)中的錶達及其臨床意義.方法 採用RT-PCR方法檢測正常宮頸、CIN、CC組織中polβ mRNA的錶達;Western Blot檢測這些組織中polβ蛋白相對錶達量.結果 正常宮頸、CIN Ⅰ、CINⅡ、CINⅢ、CC組織polβ mRNA分彆為(0.23±0.08)、(0.25 ±0.11)、(0.36±0.18)、(0.62±0.22)、(0.85±0.28);正常宮頸、CINⅡ、CINⅢ、CC組間polβ mRNA錶達差異有統計學意義(P＜0.05).正常宮頸、CIN Ⅰ、CINⅡ、CINⅢ、CC組織polβ蛋白相對錶達量分彆為(19±8)％、(18±6)％、(43±17)％、(72±26)％、(93±31)％;正常宮頸、CINⅡ、CINⅢ、CC組間pol β蛋白錶達差異有統計學意義(P＜0.05).CIN Ⅰ組織polβ mRNA和蛋白錶達與正常宮頸組織比較差異均無統計學意義(P＞0.05).結論 從CINⅡ至CCpolβ錶達逐步遞增,pol β在CC髮生髮展中起重要作用,polβ可能成為預測CC進展的生物學指標.
목적 탐토DNA취합매β(DNA polymerase beta,polβ)재궁경상피내류변(cervical intraepithelial neoplasia,CIN)화궁경암(cervical carcinoma,CC)중적표체급기림상의의.방법 채용RT-PCR방법검측정상궁경、CIN、CC조직중polβ mRNA적표체;Western Blot검측저사조직중polβ단백상대표체량.결과 정상궁경、CIN Ⅰ、CINⅡ、CINⅢ、CC조직polβ mRNA분별위(0.23±0.08)、(0.25 ±0.11)、(0.36±0.18)、(0.62±0.22)、(0.85±0.28);정상궁경、CINⅡ、CINⅢ、CC조간polβ mRNA표체차이유통계학의의(P＜0.05).정상궁경、CIN Ⅰ、CINⅡ、CINⅢ、CC조직polβ단백상대표체량분별위(19±8)％、(18±6)％、(43±17)％、(72±26)％、(93±31)％;정상궁경、CINⅡ、CINⅢ、CC조간pol β단백표체차이유통계학의의(P＜0.05).CIN Ⅰ조직polβ mRNA화단백표체여정상궁경조직비교차이균무통계학의의(P＞0.05).결론 종CINⅡ지CCpolβ표체축보체증,pol β재CC발생발전중기중요작용,polβ가능성위예측CC진전적생물학지표.
Objective To investigate the expression of pol(B) of in tissues of cervical intraepithelial neoplasia (CIN) and cervical carcinoma (CC) and its clinical significance.Methods The expression of pol(B) mRNA in normal cervical,CIN,and CC tissues were examined by RT-PCR assay,and that of pol(B)protein by Western Blot assay.Results The expressions of pol(B) mRNA of in normal cervix,CIN Ⅰ,CIN Ⅱ,CIN Ⅲ,and CC tissues were (0.23 ±0.08),(0.25 ±0.11),(0.36 ±0.18),(0.62 ±0.22),and (0.85±0.28),respectively; there were statistical differences in the expression of pol(B) mRNA between normal cervix,CIN Ⅱ,CIN Ⅲ,and CC (P＜0.05).The expressions of pol(B) protein in normal cervical,CIN Ⅰ,CIN Ⅱ,CIN Ⅲ,and CC tissues were (19±8)％,(18±6)％,(43±17)％,(72±26)％,and (93±31)％,respectively; there was statistical differences in the expression of pol(B) protein between normal cervical,CIN Ⅱ,CIN Ⅲ,and CC (P＜0.05).The expression of pol(B) mRNA and protein of in the tissues of CIN Ⅰ and normal cervix was not significantly different(P＜0.05).Conclusions The expression of pol(B) mRNA and protein increases with the progression of cervical lesions.Pol(B) might play an important role in the genesis and development of CC,and it could be the indicator in the development and prognosis of CC.
