中华神经外科杂志
中華神經外科雜誌
중화신경외과잡지
Chinese Journal of Neurosurgery
2015年
1期
76-79
,共4页
王秀%张凯%张弨%魏乃礼%王垚%刘畅%赵宝田%胡文瀚%张建国
王秀%張凱%張弨%魏迺禮%王垚%劉暢%趙寶田%鬍文瀚%張建國
왕수%장개%장초%위내례%왕요%류창%조보전%호문한%장건국
深部脑刺激法%伏核%肥胖症%胃促生长素%瘦素%神经肽Y%大鼠
深部腦刺激法%伏覈%肥胖癥%胃促生長素%瘦素%神經肽Y%大鼠
심부뇌자격법%복핵%비반증%위촉생장소%수소%신경태Y%대서
Deep brain stimulation%Nucleus accumbens%Obesity%Ghrelin%Leptin%Neuropeptide Y%Rats
目的 探讨伏隔核壳部(NAc-sh)脑深部电刺激术(DBS)对肥胖大鼠摄食量和摄食相关激素分泌的影响.方法 取8周龄雄性SD大鼠60只,高脂饮食建立肥胖大鼠模型,6个月后取24只肥胖大鼠,采用随机数字表法随机分为NAc-sh高频DBS刺激组(简称刺激组)和假刺激组,每组12只.分别在双侧NAc-sh植入刺激电极固定装置.术后30 d大鼠进食完全恢复后,两组各选取进食量稳定的大鼠10只植入电极行刺激(电压3.0V,波宽100μs,频率180 ～ 200 Hz)或假刺激,并于刺激或假刺激前后断尾取血,放射免疫方法检测外周血胃促生长素、瘦素及神经肽Y(NPY)水平的变化.结果 刺激组大鼠刺激开始摄食量即较刺激前明显下降(P＜0.05),刺激后血清瘦素和神经肽Y的浓度较刺激前明显下降[瘦素:(20±10) pg/ml对比(32±10) pg/ml;神经肽Y:(926±299) pg/ml对比(1302±287)pg/ml,P值均＜0.05],胃促生长素的血清浓度较刺激前明显增加[(1 603±848) pg/ml对比(1 066 ±310) pg/ml,P＜0.05),而假刺激组刺激前后进食量及胃促生长素、瘦素、神经肽Y的变化差异均无统计学意义(P＞0.05).结论 NAc-sh高频电刺激能有效降低肥胖大鼠摄食量,外周血激素的变化可能与外侧丘脑的兴奋性改变有关.
目的 探討伏隔覈殼部(NAc-sh)腦深部電刺激術(DBS)對肥胖大鼠攝食量和攝食相關激素分泌的影響.方法 取8週齡雄性SD大鼠60隻,高脂飲食建立肥胖大鼠模型,6箇月後取24隻肥胖大鼠,採用隨機數字錶法隨機分為NAc-sh高頻DBS刺激組(簡稱刺激組)和假刺激組,每組12隻.分彆在雙側NAc-sh植入刺激電極固定裝置.術後30 d大鼠進食完全恢複後,兩組各選取進食量穩定的大鼠10隻植入電極行刺激(電壓3.0V,波寬100μs,頻率180 ～ 200 Hz)或假刺激,併于刺激或假刺激前後斷尾取血,放射免疫方法檢測外週血胃促生長素、瘦素及神經肽Y(NPY)水平的變化.結果 刺激組大鼠刺激開始攝食量即較刺激前明顯下降(P＜0.05),刺激後血清瘦素和神經肽Y的濃度較刺激前明顯下降[瘦素:(20±10) pg/ml對比(32±10) pg/ml;神經肽Y:(926±299) pg/ml對比(1302±287)pg/ml,P值均＜0.05],胃促生長素的血清濃度較刺激前明顯增加[(1 603±848) pg/ml對比(1 066 ±310) pg/ml,P＜0.05),而假刺激組刺激前後進食量及胃促生長素、瘦素、神經肽Y的變化差異均無統計學意義(P＞0.05).結論 NAc-sh高頻電刺激能有效降低肥胖大鼠攝食量,外週血激素的變化可能與外側丘腦的興奮性改變有關.
목적 탐토복격핵각부(NAc-sh)뇌심부전자격술(DBS)대비반대서섭식량화섭식상관격소분비적영향.방법 취8주령웅성SD대서60지,고지음식건립비반대서모형,6개월후취24지비반대서,채용수궤수자표법수궤분위NAc-sh고빈DBS자격조(간칭자격조)화가자격조,매조12지.분별재쌍측NAc-sh식입자격전겁고정장치.술후30 d대서진식완전회복후,량조각선취진식량은정적대서10지식입전겁행자격(전압3.0V,파관100μs,빈솔180 ～ 200 Hz)혹가자격,병우자격혹가자격전후단미취혈,방사면역방법검측외주혈위촉생장소、수소급신경태Y(NPY)수평적변화.결과 자격조대서자격개시섭식량즉교자격전명현하강(P＜0.05),자격후혈청수소화신경태Y적농도교자격전명현하강[수소:(20±10) pg/ml대비(32±10) pg/ml;신경태Y:(926±299) pg/ml대비(1302±287)pg/ml,P치균＜0.05],위촉생장소적혈청농도교자격전명현증가[(1 603±848) pg/ml대비(1 066 ±310) pg/ml,P＜0.05),이가자격조자격전후진식량급위촉생장소、수소、신경태Y적변화차이균무통계학의의(P＞0.05).결론 NAc-sh고빈전자격능유효강저비반대서섭식량,외주혈격소적변화가능여외측구뇌적흥강성개변유관.
Objective To explore the effects of chronic high frequency deep brain stimulation (DBS) of nucleus accumbens shell subregion on food intake and regulation of appetite-related hormones.Methods High-fat diet induced obesity rats were randomly divided into two groups,namely DBS group and sham-DBS group.Stimulating electrodes were implanted in the bilateral shell subregion of nucleus accumbens.The amount of food intake was measured before and during stimulation.Peripheral concentrations of ghrelin,NPY,and leptin were tested before and after DBS or sham-DBS.Results The amount of food intake began to significantly decrease once stimulation was on.After 7 days' continuous stimulation,peripheral concentrations of NPY and leptin decreased significantly (Leptin:pre-DBS:32 ± 10 vs.post-DBS:20 ± 10pg/ml,P ＜ 0.05 ; NPY:pre-DBS:1 302 ± 287 vs.post-DBS:926 ± 299 pg/ml,P ＜ 0.05),and ghrelin increased significantly (Pre-DBS:1066 ± 310 vs.Post-DBS:1603 ± 848 pg/ml,P ＜ 0.05).Conclusions NAc shell subregion is an effective DBS target to decrease food intake in obesity rats.NAc-shell DBS seems to temporarily inhibit the hypothalamic secretion of NPY.Increase of ghrelin levels maybe a second result of decreased food intake caused by NAc-shell stimulation.
