中华器官移植杂志
中華器官移植雜誌
중화기관이식잡지
CHINESE JOURNAL OF ORGAN TRANSPLANTATION
2014年
12期
732-735
,共4页
姜然然%张建军%李延钧%王政禄%方振宇%史源
薑然然%張建軍%李延鈞%王政祿%方振宇%史源
강연연%장건군%리연균%왕정록%방진우%사원
肝移植%供者%乙型肝炎病毒
肝移植%供者%乙型肝炎病毒
간이식%공자%을형간염병독
Liver transplantation%Donor%Hepatitis B virus
目的 检测乙型肝炎病毒核心抗体(HBcAb)阳性供肝组织内乙型肝炎病毒(HBV)cccDNA,结合肝移植术后HBV复发率,探讨HBcAb阳性供肝中cccDNA与术后HBV复发的相关性.方法 回顾性研究2007年1月至2010年1月1200余例因乙型肝炎相关性疾病接受肝移植的受者资料,共筛选出受者85例.根据供肝HBV感染情况,将受者分为3组:(1)研究组:40例,供肝HBcAb阳性,但HBV表面抗原(HBsAg)阴性;(2)对照组:15例,供肝HBsAg阳性;(3)正常组:30例,供肝无HBV感染.应用荧光定量聚合酶链反应法检测供肝组织内的HBV cccDNA.定期检测受者HBV血清学及HBV DNA,如有HBsAg阳性或HBV DNA阳性者取移植肝穿刺活检确认是否复发.结果 研究组、对照组及正常组分别有分别有12例、11例和0例HBV cccDNA呈阳性,其阳性率分别为30％(12/40)、73.3％(11/15)、0.研究组、对照组及正常组术后HBV的复发率分别为10％(4/40)、80％(12/15)和3.3％(1/30).研究组中cccDNA阳性与cccDNA阴性供肝移植术后受者的HBV复发率分别为7.5％(3/40)和2.5％(1/40),二者比较,差异有统计学意义.结论 HBcAb阳性供肝中cccDNA阳性可能是肝移植术后受者HBV复发的危险因素之一.肝移植术前应对HBcAb阳性供肝进行HBV cccDNA筛选,能够减少术后HBV复发率,从而扩大供肝来源.
目的 檢測乙型肝炎病毒覈心抗體(HBcAb)暘性供肝組織內乙型肝炎病毒(HBV)cccDNA,結閤肝移植術後HBV複髮率,探討HBcAb暘性供肝中cccDNA與術後HBV複髮的相關性.方法 迴顧性研究2007年1月至2010年1月1200餘例因乙型肝炎相關性疾病接受肝移植的受者資料,共篩選齣受者85例.根據供肝HBV感染情況,將受者分為3組:(1)研究組:40例,供肝HBcAb暘性,但HBV錶麵抗原(HBsAg)陰性;(2)對照組:15例,供肝HBsAg暘性;(3)正常組:30例,供肝無HBV感染.應用熒光定量聚閤酶鏈反應法檢測供肝組織內的HBV cccDNA.定期檢測受者HBV血清學及HBV DNA,如有HBsAg暘性或HBV DNA暘性者取移植肝穿刺活檢確認是否複髮.結果 研究組、對照組及正常組分彆有分彆有12例、11例和0例HBV cccDNA呈暘性,其暘性率分彆為30％(12/40)、73.3％(11/15)、0.研究組、對照組及正常組術後HBV的複髮率分彆為10％(4/40)、80％(12/15)和3.3％(1/30).研究組中cccDNA暘性與cccDNA陰性供肝移植術後受者的HBV複髮率分彆為7.5％(3/40)和2.5％(1/40),二者比較,差異有統計學意義.結論 HBcAb暘性供肝中cccDNA暘性可能是肝移植術後受者HBV複髮的危險因素之一.肝移植術前應對HBcAb暘性供肝進行HBV cccDNA篩選,能夠減少術後HBV複髮率,從而擴大供肝來源.
목적 검측을형간염병독핵심항체(HBcAb)양성공간조직내을형간염병독(HBV)cccDNA,결합간이식술후HBV복발솔,탐토HBcAb양성공간중cccDNA여술후HBV복발적상관성.방법 회고성연구2007년1월지2010년1월1200여례인을형간염상관성질병접수간이식적수자자료,공사선출수자85례.근거공간HBV감염정황,장수자분위3조:(1)연구조:40례,공간HBcAb양성,단HBV표면항원(HBsAg)음성;(2)대조조:15례,공간HBsAg양성;(3)정상조:30례,공간무HBV감염.응용형광정량취합매련반응법검측공간조직내적HBV cccDNA.정기검측수자HBV혈청학급HBV DNA,여유HBsAg양성혹HBV DNA양성자취이식간천자활검학인시부복발.결과 연구조、대조조급정상조분별유분별유12례、11례화0례HBV cccDNA정양성,기양성솔분별위30％(12/40)、73.3％(11/15)、0.연구조、대조조급정상조술후HBV적복발솔분별위10％(4/40)、80％(12/15)화3.3％(1/30).연구조중cccDNA양성여cccDNA음성공간이식술후수자적HBV복발솔분별위7.5％(3/40)화2.5％(1/40),이자비교,차이유통계학의의.결론 HBcAb양성공간중cccDNA양성가능시간이식술후수자HBV복발적위험인소지일.간이식술전응대HBcAb양성공간진행HBV cccDNA사선,능구감소술후HBV복발솔,종이확대공간래원.
Objective To indentify the relationship between hepatitis B virus covalently closed circular DNA (HBV cccDNA) and postoperative recurrence of HBV in HBcAb positive liver donors by detecting HBV cccDNA in liver from HBcAb positive liver donors.Method Eighty-five of 1200 patients underwent liver transplantation for hepatitis B-related end-stage liver disease in our hospital from January 2007 to January 2010 were retrospectively analyzed.According to the situation of HBV infection in donor liver,the recipients were divided into 3 groups:(1) the experimental group (livers positive for HBcAb,and negative for HBsAg,n =40),(2) control group (livers positive for HBsAg,n =15),and (3) normal group (donor livers without HBV infection,n =30).HBV cccDNA of donors was detected by fluorescence quantitative PCR.Serum HBV and HBV DNA were regularly tested,and biopsy was done in those positive for HBsAg or HBV DNA to confirm HBV recurrence.The relationship between HBV cccDNA and postoperative recurrence of HBV in HBcAb positive liver donors was analyzed.Result The positive rate of cccDNA was 30％ in experimental group (12、40),73.3％ in control group (11/50),and 0 (normal group).The rate of HBV recurrence in experimental,control and normal groups was 10％ (4/40),80％ (12/15) and 3.3％ (1/30) respectively.The rate of HBV recurrence in the experimental group of cccDNA (+) and cccDNA (-) was 7.5％ (3/40),and 2.5％ (1/40).Conclusion The cccDNA in HBcAb positive donors may be one of the risk factors in HBV recurrence of hepatitis B-related liver transplantation patients.The screening of HBV cccDNA in the donor livers positive for HBcAb before liver transplantation is recommended to reduce the positive HBV recurrence and expand the pool of liver donors for patients with HBV-related liver disease.
