CAJ | 학술논문

目的探讨高迁移率蛋白A1(HMGA1)和高迁移率蛋白A2(HMGA2)表达与甲状腺乳头状癌发生、发展及转移的关系.方法收集新鲜和石蜡包埋的35例甲状腺乳头状癌(PTC)和相应的35例癌旁甲状腺组织(FT)及10例甲状腺良性病变旁正常组织(NT).采用实时荧光定量PCR及免疫组化法分别检测HMGA1和HMGA2 mRNA及蛋白的表达水平.结果 HMGA1和HMGA2 mRNA在NT组几乎都不表达,二者在PT组表达较NT组差异无统计学意义(P＞0.05),二者在PTC组表达为NT组的173.5倍和5.9倍(均P＜0.01).HMGA1和HMGA2蛋白分别在NT组、PT组及PTC组的表达水平逐步升高,组间比较有统计学意义(均P＜0.01);PTC组中HMGA1和HMGA2蛋白表达水平分别在临床分期和淋巴结有无转移比较中有统计学意义(P＜0.05或P＜0.01),分别与患者的年龄、肿瘤大小、性别比较差异均无统计学意义(P＞0.05).PTC组中HMGA1 mRNA和蛋白的表达水平都较HMGA2显著升高(P＜0.05或P＜0.01).结论 HMGA1和HMGA2过表达与PTC的发生、发展和转移有关.相对于HMGA2,HMGA1是鉴别甲状腺良恶性病变更好的分子指标.
목적 탐토고천이솔단백A1(HMGA1)화고천이솔단백A2(HMGA2)표체여갑상선유두상암발생、발전급전이적관계.방법 수집신선화석사포매적35례갑상선유두상암(PTC)화상응적35례암방갑상선조직(FT)급10례갑상선량성병변방정상조직(NT).채용실시형광정량PCR급면역조화법분별검측HMGA1화HMGA2 mRNA급단백적표체수평.결과 HMGA1화HMGA2 mRNA재NT조궤호도불표체,이자재PT조표체교NT조차이무통계학의의(P＞0.05),이자재PTC조표체위NT조적173.5배화5.9배(균P＜0.01).HMGA1화HMGA2단백분별재NT조、PT조급PTC조적표체수평축보승고,조간비교유통계학의의(균P＜0.01);PTC조중HMGA1화HMGA2단백표체수평분별재림상분기화림파결유무전이비교중유통계학의의(P＜0.05혹P＜0.01),분별여환자적년령、종류대소、성별비교차이균무통계학의의(P＞0.05).PTC조중HMGA1 mRNA화단백적표체수평도교HMGA2현저승고(P＜0.05혹P＜0.01).결론 HMGA1화HMGA2과표체여PTC적발생、발전화전이유관.상대우HMGA2,HMGA1시감별갑상선량악성병변경호적분자지표.
Objective To study the relationship of high mobility group A (HMGA) 1 and HMGA2 expressions with the occurrence,development,and metastasis of papillary thyroid carcinoma.Methods Fresh and paraffin-embedded 35 cases of papillary thyroid carcinoma tissues (PTC) and corresponding paracancerous thyroid tissues (PT),and 10 cases of normal tissues (NT) from adjacent thyroid benign lesions were collected.The mRNA and protein expressions of HMGA1 and HMGA2 were detected by realtime PCR and immunohistochemistry respectively.Results The mRNA expressions of HMGA1 and HMGA2 showed nearly no expression in the NT group,no significant difference between PT group and NT group(P＞0.05),and significantly higher expression in PTC group compared with NT group (173.5 folds and 5.9 folds,both P ＜0.01).The protein expressions of HMGA1 and HMGA2 gradually increased in NT group,PT group,and PTC group,with significant difference between these groups(both P＜0.01).The protein expressions of HMGA1 and HMGA2 in PTC group with various clinical stages or with and without lymph node metastasis showed statistically significant difference (P ＜0.05 or P ＜0.01),but it did not show any significant difference regarding patients' age,gender,and tumor size(P＞0.05).The mRNA and protein expressions of HMGA1 were significantly higher than those of HMGA2 in PTC group(P＜0.05 or P＜0.01).Conclusions The overexpressions of HMGA1 and HMGA2 were related to development and metastasis of papillary thyroid carcinoma.Compared with HMGA2,HMGA1 was a better molecular marker in identifying benign and malignant thyroid tumors.