中华泌尿外科杂志
中華泌尿外科雜誌
중화비뇨외과잡지
CHINESE JOURNAL OF UROLOGY
2015年
2期
108-112
,共5页
脊髓损伤%逼尿肌过度活动%单纯疱疹病毒%尿氨酸氨基转移酶%大鼠,Wistar
脊髓損傷%逼尿肌過度活動%單純皰疹病毒%尿氨痠氨基轉移酶%大鼠,Wistar
척수손상%핍뇨기과도활동%단순포진병독%뇨안산안기전이매%대서,Wistar
Spinal cord injuries%Detrusor overactivity%Herpes simplex virus%Kynurenine aminotransferase%Rats,Wistar
目的 探讨膀胱壁注射复制缺陷单纯疱疹病毒介导的尿氨酸氨基转移酶Ⅱ(herpes simplex virus mediated kynurenine aminotransferaseⅡ,HSV-KATⅡ)对脊髓损伤大鼠逼尿肌过度活动(detrusor overactivity,DO)的影响. 方法 2012年6月至2013年7月将48只雄性Wistar大鼠T10脊髓横断,按随机数字法分为3组:生理盐水组、单纯疱疹载体组(HSV组)和HSV-KATⅡ组,每组16只.脊髓横断1周后分别膀胱壁注射生理盐水(40μl)、HSV和HSV-KATⅡ(各40μl,病毒总量1×107空斑形成单位),注射3周后进行膀胱测压、L6～S1背根神经节病毒转染效率和KATⅡ基因表达检测. 结果 与生理盐水组和HSV组相比,HSV-KATⅡ组大鼠DO的数量和幅度、最大漏尿点压力、漏尿点膀胱容积分别下降了59.6％～61.1％、21.6％ ～ 24.2％、30.3％ ～ 34.4％和44.1％ ～ 46.5％,排尿效率增加了40.7％ ～47.7％,第一次DO前的灌注时间延长了30.1％～49.0％,HSV-KATⅡ组与其他两组间比较差异均有统计学意义(P＜0.01),但是,3组间漏尿量比较差异无统计学意义(P＞0.05).HSV组和HSV-KATⅡ组L6～S1背根神经节KATⅡ蛋白相对表达量分别为0.28±0.07和0.50±0.13,KATⅡmRNA相对表达量分别为0.51±0.08和0.78±0.06,组间比较差异均有统计学意义(P＜0.05;P＜0.01).结论 膀胱壁注射HSV-KATⅡ能够抑制脊髓损伤大鼠DO,并可能改善逼尿肌-括约肌协同失调.
目的 探討膀胱壁註射複製缺陷單純皰疹病毒介導的尿氨痠氨基轉移酶Ⅱ(herpes simplex virus mediated kynurenine aminotransferaseⅡ,HSV-KATⅡ)對脊髓損傷大鼠逼尿肌過度活動(detrusor overactivity,DO)的影響. 方法 2012年6月至2013年7月將48隻雄性Wistar大鼠T10脊髓橫斷,按隨機數字法分為3組:生理鹽水組、單純皰疹載體組(HSV組)和HSV-KATⅡ組,每組16隻.脊髓橫斷1週後分彆膀胱壁註射生理鹽水(40μl)、HSV和HSV-KATⅡ(各40μl,病毒總量1×107空斑形成單位),註射3週後進行膀胱測壓、L6～S1揹根神經節病毒轉染效率和KATⅡ基因錶達檢測. 結果 與生理鹽水組和HSV組相比,HSV-KATⅡ組大鼠DO的數量和幅度、最大漏尿點壓力、漏尿點膀胱容積分彆下降瞭59.6％～61.1％、21.6％ ～ 24.2％、30.3％ ～ 34.4％和44.1％ ～ 46.5％,排尿效率增加瞭40.7％ ～47.7％,第一次DO前的灌註時間延長瞭30.1％～49.0％,HSV-KATⅡ組與其他兩組間比較差異均有統計學意義(P＜0.01),但是,3組間漏尿量比較差異無統計學意義(P＞0.05).HSV組和HSV-KATⅡ組L6～S1揹根神經節KATⅡ蛋白相對錶達量分彆為0.28±0.07和0.50±0.13,KATⅡmRNA相對錶達量分彆為0.51±0.08和0.78±0.06,組間比較差異均有統計學意義(P＜0.05;P＜0.01).結論 膀胱壁註射HSV-KATⅡ能夠抑製脊髓損傷大鼠DO,併可能改善逼尿肌-括約肌協同失調.
목적 탐토방광벽주사복제결함단순포진병독개도적뇨안산안기전이매Ⅱ(herpes simplex virus mediated kynurenine aminotransferaseⅡ,HSV-KATⅡ)대척수손상대서핍뇨기과도활동(detrusor overactivity,DO)적영향. 방법 2012년6월지2013년7월장48지웅성Wistar대서T10척수횡단,안수궤수자법분위3조:생리염수조、단순포진재체조(HSV조)화HSV-KATⅡ조,매조16지.척수횡단1주후분별방광벽주사생리염수(40μl)、HSV화HSV-KATⅡ(각40μl,병독총량1×107공반형성단위),주사3주후진행방광측압、L6～S1배근신경절병독전염효솔화KATⅡ기인표체검측. 결과 여생리염수조화HSV조상비,HSV-KATⅡ조대서DO적수량화폭도、최대루뇨점압력、루뇨점방광용적분별하강료59.6％～61.1％、21.6％ ～ 24.2％、30.3％ ～ 34.4％화44.1％ ～ 46.5％,배뇨효솔증가료40.7％ ～47.7％,제일차DO전적관주시간연장료30.1％～49.0％,HSV-KATⅡ조여기타량조간비교차이균유통계학의의(P＜0.01),단시,3조간루뇨량비교차이무통계학의의(P＞0.05).HSV조화HSV-KATⅡ조L6～S1배근신경절KATⅡ단백상대표체량분별위0.28±0.07화0.50±0.13,KATⅡmRNA상대표체량분별위0.51±0.08화0.78±0.06,조간비교차이균유통계학의의(P＜0.05;P＜0.01).결론 방광벽주사HSV-KATⅡ능구억제척수손상대서DO,병가능개선핍뇨기-괄약기협동실조.
Objective To evaluate if replication-defective herpes simplex virus mediated kynurenine aminotransferase Ⅱ (HSV-KAT Ⅱ) could inhibit detrusor overactivity (DO) in rats with spinal cord injury by bladder wall injection.Methods From June 2012 to July 2013,48 male Wistar rats with T10 spinal cord transection (SCT) were randomly divided into normal saline group,HSV group and HSV-KAT Ⅱ group (n=16 each group).Normal saline (40 μl),HSV or HSV-KAT Ⅱ (40 μl,1×107 plaque forming unit viruses) was injected into the rat bladder wall of according group by 1 week after SCT.Three weeks after injection,cystometry was performed and the virus transfected efficiency,expression of KAT Ⅱ in L6-S1 dorsal root ganglia were examined.Results The DO number,DO amplitude,maximum voiding pressure and volume inducing voiding were decreased significantly by 59.6％-61.1％,21.6％-24.2％,30.3％-34.4％ and 44.1％-46.5％ (P＜0.01),while voiding efficiency,the time to first DO were increased significantly by 40.7％-47.7％ and 30.1％-49.0％ (P＜0.01) in the HSV-KAT Ⅱ group compared with normal saline group and HSV group.However,the leaking volumes were no significantly different among the 3 groups (P＞0.05).The relative intensity of KAT Ⅱ protein (0.50±0.13 versus 0.28±0.07,P＜0.05) and mRNA (0.78±0.06 versus 0.51±0.08,P＜0.01) were increased significantly in HSV-KAT Ⅱ group than those in HSV group.Conclusion HSV-KATⅡ bladder wall injection inhibits DO and may improve detrusor-sphincter dyssynergia in rats with spinal cord injury.
