中华口腔医学杂志
中華口腔醫學雜誌
중화구강의학잡지
Chinese Journal of Stomatology
2015年
1期
23-27
,共5页
扁平苔藓,口腔%单核细胞%血浆%微RNAs
扁平苔蘚,口腔%單覈細胞%血漿%微RNAs
편평태선,구강%단핵세포%혈장%미RNAs
Lichen planus,oral%Monocytes%Plasma%MicroRNAs
目的 探讨口腔扁平苔藓(oral lichen planus,OLP)患者外周血单个核细胞(peripheralbloodmononuclearcell,PBMC)和血浆中微小RNA (micro RNA,miRNA) 155 (miRNA-155)及miRNA-146a的表达,阐明其在OLP发病中的作用及意义.方法 收集2012年1月至2013年5月南昌大学附属口腔医院口腔内科收治的OLP患者32例(OLP组),其中女性25例,男性7例,年龄25～54岁.所有OLP患者均为初诊且经病理学确诊,按照病损基部黏膜状况又分为非糜烂型(18例)和糜烂型(14例)OLP.选择20名性别、年龄匹配的健康志愿者作为健康对照组.采用实时荧光定量PCR技术检测两组受试者PBMC及血浆中miRNA-155、miRNA-146a的表达水平并进行统计学分析.结果 OLP组PBMC和血浆中miRNA-155的表达水平(中位数分别为0.07和5.84)均显著高于健康对照组(中位数分别为0.03和1.32),差异均有统计学意义(P＜0.05);OLP患者PBMC和血浆中miRNA-146a的表达水平(中位数分别为1.26和412.60)均显著高于健康对照组(中位数分别为0.58和238.42),差异均有统计学意义(P＜0.05).糜烂型OLP组血浆中miRNA-155和miRNA-146a的表达均显著高于非糜烂型OLP组,差异均有统计学意义(P＜0.05);PBMC中miRNA-155和miRNA-146a的表达与非糜烂型OLP组比较,差异均无统计学意义(P＞0.05).结论 OLP患者PBMC和血浆中miRNA-155、miRNA-146a的表达水平显著升高,且血浆中miRNA-155和miRNA-146a的表达变化与OLP病损严重程度有关,提示miRNA-155和miRNA-146a在OLP的发病中起一定作用.
目的 探討口腔扁平苔蘚(oral lichen planus,OLP)患者外週血單箇覈細胞(peripheralbloodmononuclearcell,PBMC)和血漿中微小RNA (micro RNA,miRNA) 155 (miRNA-155)及miRNA-146a的錶達,闡明其在OLP髮病中的作用及意義.方法 收集2012年1月至2013年5月南昌大學附屬口腔醫院口腔內科收治的OLP患者32例(OLP組),其中女性25例,男性7例,年齡25～54歲.所有OLP患者均為初診且經病理學確診,按照病損基部黏膜狀況又分為非糜爛型(18例)和糜爛型(14例)OLP.選擇20名性彆、年齡匹配的健康誌願者作為健康對照組.採用實時熒光定量PCR技術檢測兩組受試者PBMC及血漿中miRNA-155、miRNA-146a的錶達水平併進行統計學分析.結果 OLP組PBMC和血漿中miRNA-155的錶達水平(中位數分彆為0.07和5.84)均顯著高于健康對照組(中位數分彆為0.03和1.32),差異均有統計學意義(P＜0.05);OLP患者PBMC和血漿中miRNA-146a的錶達水平(中位數分彆為1.26和412.60)均顯著高于健康對照組(中位數分彆為0.58和238.42),差異均有統計學意義(P＜0.05).糜爛型OLP組血漿中miRNA-155和miRNA-146a的錶達均顯著高于非糜爛型OLP組,差異均有統計學意義(P＜0.05);PBMC中miRNA-155和miRNA-146a的錶達與非糜爛型OLP組比較,差異均無統計學意義(P＞0.05).結論 OLP患者PBMC和血漿中miRNA-155、miRNA-146a的錶達水平顯著升高,且血漿中miRNA-155和miRNA-146a的錶達變化與OLP病損嚴重程度有關,提示miRNA-155和miRNA-146a在OLP的髮病中起一定作用.
목적 탐토구강편평태선(oral lichen planus,OLP)환자외주혈단개핵세포(peripheralbloodmononuclearcell,PBMC)화혈장중미소RNA (micro RNA,miRNA) 155 (miRNA-155)급miRNA-146a적표체,천명기재OLP발병중적작용급의의.방법 수집2012년1월지2013년5월남창대학부속구강의원구강내과수치적OLP환자32례(OLP조),기중녀성25례,남성7례,년령25～54세.소유OLP환자균위초진차경병이학학진,안조병손기부점막상황우분위비미란형(18례)화미란형(14례)OLP.선택20명성별、년령필배적건강지원자작위건강대조조.채용실시형광정량PCR기술검측량조수시자PBMC급혈장중miRNA-155、miRNA-146a적표체수평병진행통계학분석.결과 OLP조PBMC화혈장중miRNA-155적표체수평(중위수분별위0.07화5.84)균현저고우건강대조조(중위수분별위0.03화1.32),차이균유통계학의의(P＜0.05);OLP환자PBMC화혈장중miRNA-146a적표체수평(중위수분별위1.26화412.60)균현저고우건강대조조(중위수분별위0.58화238.42),차이균유통계학의의(P＜0.05).미란형OLP조혈장중miRNA-155화miRNA-146a적표체균현저고우비미란형OLP조,차이균유통계학의의(P＜0.05);PBMC중miRNA-155화miRNA-146a적표체여비미란형OLP조비교,차이균무통계학의의(P＞0.05).결론 OLP환자PBMC화혈장중miRNA-155、miRNA-146a적표체수평현저승고,차혈장중miRNA-155화miRNA-146a적표체변화여OLP병손엄중정도유관,제시miRNA-155화miRNA-146a재OLP적발병중기일정작용.
Objective To investigate the expression and clinical significance of miRNA-155 and miRNA-146a in peripheral blood mononuclear cells(PBMC) and plasma of oral lichen planus(OLP) patients.Methods Twenty-five female and seven male OLP patients(OLP group) aged 25 to 54 years were selected from January 2012 to May 2013.The diagnosis was confirmed by pathology and the lesions were divided into two non-erosive OLP group(18 cases) and erosive OLP group(14 cases).Twenty healthy sex and age matched volunteers served as control,miRNA-155 and miRNA-146a expressions in PBMC and plasma were examined by real-time PCR.The difference between OLP group and control group was statistically analyzed.Results The expressions of PBMC and plasma miRNA-155 were higher in OLP patients than those in the healthy control (median,0.07 vs 0.03,P＜0.05; 5.84 vs 1.32,P＜0.01).The median expression level of miRNA-146a in PBMC and plasma of OLP patients and healthy controls were (1.26 vs 0.58,P＜0.05) and (412.60 vs 238.42,P＜0.01).The plasma miRNA-155 and miRNA-146a expressions were significantly higher in erosive OLP group than those in non-erosive OLP group.There were no significant differences in the expression of PBMC miRNA-155 and miRNA-146a between the two groups.Conclusions The expressions of PBMC and plasma miRNA-155 and miRNA-146a are higher in OLP patients.The expressions of plasma miRNA-155 and miRNA-146a are associated with OLP severity.The over expression of miRNA-155 and miRNA-146a in OLP may play a role in the pathogenesis of OLP.
