CAJ | 학술논문

目的研究鼠胚胎颌面组织中外胚间充质干细胞(ectomesenchymal stem cells,EMSC)的生物学特性,探讨p75+EMSC的体外分化及影响因素,为揭示牙齿发生、发育机制提供实验依据.方法通过提取12.5 d SD鼠胚胎颌面组织,用免疫荧光染色方法显示EMSC的迁移位置;用p75神经营养因子受体标记EMSC,通过流式细胞技术分选出p75+EMSC,并检测细胞周期和干细胞相关细胞表面抗原.结果免疫荧光染色结果说明颅神经嵴源性干细胞在鼠胚胎12.5 d迁移到颌突中.p75+EMSC的阳性分选率为6.1％,细胞呈均匀成纤维细胞形态,生长曲线呈“S”型,在传代过程中S期的比例稳定.细胞特异性标志物CD29、CD146、Stro-1标记p75+EMSC,其在p75+EMSC中的表达均较高(＞90％).结论 SD鼠受孕12.5 d时,胚胎的牙齿启动尚未发生;分选后的p75+EMSC在传代过程中具有稳定的增殖能力和干细胞特征,尚未开始分化.
목적 연구서배태합면조직중외배간충질간세포(ectomesenchymal stem cells,EMSC)적생물학특성,탐토p75+EMSC적체외분화급영향인소,위게시아치발생、발육궤제제공실험의거.방법 통과제취12.5 d SD서배태합면조직,용면역형광염색방법현시EMSC적천이위치;용p75신경영양인자수체표기EMSC,통과류식세포기술분선출p75+EMSC,병검측세포주기화간세포상관세포표면항원.결과 면역형광염색결과설명로신경척원성간세포재서배태12.5 d천이도합돌중.p75+EMSC적양성분선솔위6.1％,세포정균균성섬유세포형태,생장곡선정“S”형,재전대과정중S기적비례은정.세포특이성표지물CD29、CD146、Stro-1표기p75+EMSC,기재p75+EMSC중적표체균교고(＞90％).결론 SD서수잉12.5 d시,배태적아치계동상미발생;분선후적p75+EMSC재전대과정중구유은정적증식능력화간세포특정,상미개시분화.
Objective To investigate the biological characteristic of ectomesenchymal stem cells (EMSC) and the differentiation of p75+EMSC and influencing factors.Methods The immunohistochemical staining method was used to observe the migration of EMSC from 12.5 d SD rat embryonic facial process.Then EMSC was labeled by p75 neurotrophin receptor,and the cell cycle and stem cell surface antigens of the p75 + EMSC was examined.Results Immunohistochemical staining showed that stem cells from the cranial neural crest migrated to embryonic rat facial process at 12.5 days.The sorting rate of the p75+EMSC was 6.1％.The proportion of the p75 +EMSCs' S/G2/M phase was stable during subculture.The special substances CD29,CD146 and Stro-1 were marked for p75 +EMSC,and the expressions of the markers were all higher(＞90％) in p75+EMSC.Conclusions The embryonic tooth did not start to grow on the conception 12.5 days of SD rats.The p75 + EMSC after sorting had stable proliferation ability and had stem cell characteristics during subculture and didn't start differentiation.