中华结核和呼吸杂志
中華結覈和呼吸雜誌
중화결핵화호흡잡지
Chinese Journal of Tuberculosis and Respiratory Diseases
2015年
1期
39-44
,共6页
莫碧文%李洁%韦江红%王昌明%曾锦荣%王绩英%黄剑伟
莫碧文%李潔%韋江紅%王昌明%曾錦榮%王績英%黃劍偉
막벽문%리길%위강홍%왕창명%증금영%왕적영%황검위
哮喘%因子,SDF-1%受体,CXCR4
哮喘%因子,SDF-1%受體,CXCR4
효천%인자,SDF-1%수체,CXCR4
Asthma%Factor,SDF-1%Receptor,CXCR4
目的 探讨基质细胞衍生因子-1(SDF-1)、CXC趋化因子受体4(CXCR4)在支气管哮喘(哮喘)大鼠气道炎症及气道重塑中的作用.方法 将18只SPF级SD雌性大鼠按随机数字表法随机分为对照组、哮喘4周组和哮喘8周组,每组6只.卵清白蛋白(OVA)致敏后,雾化吸人OVA制作哮喘模型.哮喘模型成功后,测定气道压力;通过HE染色、Image-Pro Plus图像分析软件分析大鼠气道平滑肌的嗜酸粒细胞浸润情况,测定支气管管腔的内周长、管壁面积、支气管平滑肌面积以及平滑肌细胞核数;RT-PCR、Western blot方法检测各组大鼠肺组织SDF-1、CXCR4的表达变化;免疫组织化学法检测各组大鼠气道壁SDF-1表达的变化;统计数据并分析SDF-1、CXCR4与哮喘气道重塑及气道炎症的关系.结果 哮喘4周组、哮喘8周组大鼠的气道反应性、气道壁嗜酸粒细胞计数、支气管管壁面积、支气管平滑肌面积、平滑肌细胞核数目均明显高于对照组,哮喘两组间上述指标差异均有统计学意义(均P＜0.01);RT-PCR检测结果显示,哮喘4周组、哮喘8周组大鼠肺组织SDF-1(分别为0.583±0.004和0.724±0.008)、CXCR4(分别为0.467±0.003和0.655±0.002)的表达明显高于对照组(SDF-1为0.146 ±0.003、CXCR4为0.281±0.002),哮喘8周组的SDF-1及CXCR4的表达亦明显高于哮喘4周组,差异均有统计学意义Western blot检测结果显示,(均P＜0.01).Western blot检测结果显示,哮喘4周组、哮喘8周组大鼠气道壁SDF-1的表达(分别为0.270 ±0.006和0.350±0.009)明显高于对照组(0.180±0.009),哮喘8周组的SDF-1的表达量亦高于哮喘4周组,差异均有统计学意义(均P＜0.01);各组大鼠肺组织、气道壁SDF-1、CXCR4mRNA及蛋白的表达与气道反应性、嗜酸粒细胞浸润数、支气管壁面积、支气管平滑肌厚度及支气管平滑肌细胞核数均呈正相关(均P＜0.01).结论 SDF-1/CXCR4信号轴可能在哮喘气道炎症及气道重塑病理过程中起重要作用.
目的 探討基質細胞衍生因子-1(SDF-1)、CXC趨化因子受體4(CXCR4)在支氣管哮喘(哮喘)大鼠氣道炎癥及氣道重塑中的作用.方法 將18隻SPF級SD雌性大鼠按隨機數字錶法隨機分為對照組、哮喘4週組和哮喘8週組,每組6隻.卵清白蛋白(OVA)緻敏後,霧化吸人OVA製作哮喘模型.哮喘模型成功後,測定氣道壓力;通過HE染色、Image-Pro Plus圖像分析軟件分析大鼠氣道平滑肌的嗜痠粒細胞浸潤情況,測定支氣管管腔的內週長、管壁麵積、支氣管平滑肌麵積以及平滑肌細胞覈數;RT-PCR、Western blot方法檢測各組大鼠肺組織SDF-1、CXCR4的錶達變化;免疫組織化學法檢測各組大鼠氣道壁SDF-1錶達的變化;統計數據併分析SDF-1、CXCR4與哮喘氣道重塑及氣道炎癥的關繫.結果 哮喘4週組、哮喘8週組大鼠的氣道反應性、氣道壁嗜痠粒細胞計數、支氣管管壁麵積、支氣管平滑肌麵積、平滑肌細胞覈數目均明顯高于對照組,哮喘兩組間上述指標差異均有統計學意義(均P＜0.01);RT-PCR檢測結果顯示,哮喘4週組、哮喘8週組大鼠肺組織SDF-1(分彆為0.583±0.004和0.724±0.008)、CXCR4(分彆為0.467±0.003和0.655±0.002)的錶達明顯高于對照組(SDF-1為0.146 ±0.003、CXCR4為0.281±0.002),哮喘8週組的SDF-1及CXCR4的錶達亦明顯高于哮喘4週組,差異均有統計學意義Western blot檢測結果顯示,(均P＜0.01).Western blot檢測結果顯示,哮喘4週組、哮喘8週組大鼠氣道壁SDF-1的錶達(分彆為0.270 ±0.006和0.350±0.009)明顯高于對照組(0.180±0.009),哮喘8週組的SDF-1的錶達量亦高于哮喘4週組,差異均有統計學意義(均P＜0.01);各組大鼠肺組織、氣道壁SDF-1、CXCR4mRNA及蛋白的錶達與氣道反應性、嗜痠粒細胞浸潤數、支氣管壁麵積、支氣管平滑肌厚度及支氣管平滑肌細胞覈數均呈正相關(均P＜0.01).結論 SDF-1/CXCR4信號軸可能在哮喘氣道炎癥及氣道重塑病理過程中起重要作用.
목적 탐토기질세포연생인자-1(SDF-1)、CXC추화인자수체4(CXCR4)재지기관효천(효천)대서기도염증급기도중소중적작용.방법 장18지SPF급SD자성대서안수궤수자표법수궤분위대조조、효천4주조화효천8주조,매조6지.란청백단백(OVA)치민후,무화흡인OVA제작효천모형.효천모형성공후,측정기도압력;통과HE염색、Image-Pro Plus도상분석연건분석대서기도평활기적기산립세포침윤정황,측정지기관관강적내주장、관벽면적、지기관평활기면적이급평활기세포핵수;RT-PCR、Western blot방법검측각조대서폐조직SDF-1、CXCR4적표체변화;면역조직화학법검측각조대서기도벽SDF-1표체적변화;통계수거병분석SDF-1、CXCR4여효천기도중소급기도염증적관계.결과 효천4주조、효천8주조대서적기도반응성、기도벽기산립세포계수、지기관관벽면적、지기관평활기면적、평활기세포핵수목균명현고우대조조,효천량조간상술지표차이균유통계학의의(균P＜0.01);RT-PCR검측결과현시,효천4주조、효천8주조대서폐조직SDF-1(분별위0.583±0.004화0.724±0.008)、CXCR4(분별위0.467±0.003화0.655±0.002)적표체명현고우대조조(SDF-1위0.146 ±0.003、CXCR4위0.281±0.002),효천8주조적SDF-1급CXCR4적표체역명현고우효천4주조,차이균유통계학의의Western blot검측결과현시,(균P＜0.01).Western blot검측결과현시,효천4주조、효천8주조대서기도벽SDF-1적표체(분별위0.270 ±0.006화0.350±0.009)명현고우대조조(0.180±0.009),효천8주조적SDF-1적표체량역고우효천4주조,차이균유통계학의의(균P＜0.01);각조대서폐조직、기도벽SDF-1、CXCR4mRNA급단백적표체여기도반응성、기산립세포침윤수、지기관벽면적、지기관평활기후도급지기관평활기세포핵수균정정상관(균P＜0.01).결론 SDF-1/CXCR4신호축가능재효천기도염증급기도중소병리과정중기중요작용.
Objective To explore the roles of stromal cell-derived factor 1 (SDF-1) and C-X-C chemokine receptor 4 (CXCR4) on airway inflammation and airway remodeling in rat asthma models.Methods Eighteen female SD rats were randomly divided into 3 groups (n =6):control group,asthmatic 4 weeks group and asthmatic 8 weeks group.The rats were sensitized and inhaled ovalbumin (OVA).After the asthma model was successfully established,the airway pressure was measured.The methods of HE staining and Image-Pro Plus image analysis software were used to detect the changes of eosinophils (EOS),the perimeter of inner bronchial lumen,the wall area,the area of bronchial smooth muscle and the number of smooth muscle cells of airway walls.RT-PCR and Western-blot were used to detect the expression of SDF-1 and CXCR4 in lung tissues among the 3 groups.Immunohistochemistry was used to detect the expression of SDF-1 in airway walls.Results Compared with the control group,the airway responsiveness,the count of EOS,the area of bronchial wall,the area of bronchial smooth muscle,the number of smooth muscle cells of airway walls in the asthmatic 4 weeks and asthmatic 8 weeks were significantly increased,and significant difference between the 2 asthmatic groups was also observed in the above indexes(P ＜ 0.01).RT-PCR showed that compared with the control group (SDF-1 was 0.146 ± 0.003 and CXCR4 was 0.281 ±0.002),the expression of SDF-1 (0.583 ±0.004 and 0.724 ±0.008) and CXCR4 (0.467 ± 0.003 and 0.655 ± 0.002) in lung tissues in the asthmatic 4 weeks and asthmatic 8 weeks were significantly increased (P ＜ 0.01).In addition,compared with the asthmatic 4 weeks group,the expression of SDF-1 and CXCR4 in lung tissues in the 8 weeks asthmatic group were significantly increased (P ＜0.01).Compared with the control group(0.180 ±0.009),the expression of SDF-1 in airway walls in the asthmatic 4 weeks and asthmatic 8 weeks groups(0.270 ±0.006 and 0.350 ±0.009) were significantly increased(P ＜ 0.01).In addition,compared with the asthmatic 4 weeks group,the expression of SDF-1 in airway walls in the 8 weeks asthmatic group was significantly increased(P ＜ 0.01).The expression of SDF-1 and CXCR4 was correlated positively with the airway responsiveness,the number of EOS,the area of bronchial wall,the area of bronchial smooth muscle and the number of smooth muscle cells of airway walls (P ＜ 0.01).Conclusions SDF-1/CXCR4 axis may play a key role in airway inflammation and airway remodeling of asthma.
