CAJ | 학술논문

目的探索实验性肝纤维化血管新生的评价方法. 方法将C57BL/6雄性小鼠随机分为正常组和模型组,每组6只,以体积分数10％ CCl4橄榄油溶液腹腔注射4周制作肝纤维化模型.分别以MRI、CT、同步辐射X线二维成像及vWF免疫荧光染色方法在不同层面观察肝脏血管;HE及天狼猩红染色观察肝组织炎症及纤维化程度.两组比较采用t检验;MRI信号强度、CT值、微血管相对长度与微血管密度(MVD)的相关性采用Pearson相关分析. 结果与正常组比较,模型组小鼠肝组织炎症和纤维化明显;MR示T2WI上两组增强前后信号强度均减低,且增强后两组之间信号强度差异有统计学意义(386.67±69.04对比492.67±112.50,t=-2.456,P=0.026);CT示模型组增强后肝脏CT值及增强前后差值均较正常组显著降低;同步辐射X线二维成像后,模型组纤维化肝脏血管纹理增多、紊乱,可见小血管数量较多;MR在T2W增强的信号值(439.67±104.80,Pearson相关系数为0.714,P=0.009)、微血管相对长度(676.53±122.75,Pearson相关系数为0.791,P=0.002)与vWF标记MVD (14.50±5.95)呈正相关,CT增强前后的差值(132.60±57.02,Pearson相关系数为-0.612,P=0.034)与MVD呈负相关.结论采用纳米对比剂的MR、CT检测及同步辐射X线二维成像可用于纤维化血管新生的评价,与传统的MVD法具有相关性;其中同步辐射X线二维成像更直观、清晰,是评价肝血管新生的良好方法.
목적 탐색실험성간섬유화혈관신생적평개방법. 방법 장C57BL/6웅성소서수궤분위정상조화모형조,매조6지,이체적분수10％ CCl4감람유용액복강주사4주제작간섬유화모형.분별이MRI、CT、동보복사X선이유성상급vWF면역형광염색방법재불동층면관찰간장혈관;HE급천랑성홍염색관찰간조직염증급섬유화정도.량조비교채용t검험;MRI신호강도、CT치、미혈관상대장도여미혈관밀도(MVD)적상관성채용Pearson상관분석. 결과 여정상조비교,모형조소서간조직염증화섬유화명현;MR시T2WI상량조증강전후신호강도균감저,차증강후량조지간신호강도차이유통계학의의(386.67±69.04대비492.67±112.50,t=-2.456,P=0.026);CT시모형조증강후간장CT치급증강전후차치균교정상조현저강저;동보복사X선이유성상후,모형조섬유화간장혈관문리증다、문란,가견소혈관수량교다;MR재T2W증강적신호치(439.67±104.80,Pearson상관계수위0.714,P=0.009)、미혈관상대장도(676.53±122.75,Pearson상관계수위0.791,P=0.002)여vWF표기MVD (14.50±5.95)정정상관,CT증강전후적차치(132.60±57.02,Pearson상관계수위-0.612,P=0.034)여MVD정부상관.결론 채용납미대비제적MR、CT검측급동보복사X선이유성상가용우섬유화혈관신생적평개,여전통적MVD법구유상관성;기중동보복사X선이유성상경직관、청석,시평개간혈관신생적량호방법.
Objective To investigate the methods for assessing angiogenesis in experimental liver fibrosis.Methods Male C57BL/6 mice were randomly divided into a normal (unmodeled) group and a liver fibrosis model group.The model was established by a 4-week course of 10％ CCl4 solution (in olive oil) intraperitoneal injection.Liver vasculature was observed by magnetic resonance (MR),computed tomography (CT),synchrotron radiation X-ray,and von Willibrand factor (vWF) immunofluorescence staining.Liver inflammation and fibrosis were observed by staining with hematoxylin-eosin and Sirius red.The t-test and Pearson's correlation analysis were used for statistical analyses.Results Compared with the normal group,the model group had more robust inflammation and fibrosis in liver tissue.The liver tissue in the modeled mice showed significant deceases in MR signal intensity on T2WI before and after enhancement (386.67±69.04 vs.492.67±112.50,t =-2.456,P =0.026).The liver tissue in the modeled mice also showed significantly decreased enhancement CT values (P ＜ 0.01).Synchrotron radiation X-ray imaging showed that the small vessels in the liver tissues of the modeled mice were significantly increased compared to that in the normal mice (P ＜ 0.01).The MR T2W enhanced signal value (439.67±104.80,Pearson's r =0.714,P =0.009) and microvascular relative length (676.53±122.75,Pearson's r =0.791,P =0.002) were positive correlated with microvessel density (MVD) (14.50±5.95),as shown by detection of labeled vWF.The before and after CT enhancement difference (132.60±57.02,Pearson's r =-0.612,P =0.034) was negatively correlated with MVD.Conclusions MR,CT and synchrotron radiation X-ray imaging can be used for assessing angiogenesis in liver fibrosis,and the findings from each are correlated with the traditional MVD detection method.The two-dimensional imaging of synchrotron radiation X-ray is more intuitive,and has been confirmed as an effective evaluation method for liver angiogenesis.