CAJ | 학술논문

目的观察Tat-Smac N7融合肽对人肺癌细胞系H460和人食管癌细胞系EC109的辐射增敏作用,探讨其辐射增敏机制.方法取对数生长期H460和EC109细胞,分为DAPI对照组、FITC-Smac N7和FITC-Tat-Smac N7组,荧光显微镜观察两种细胞不同时间药物入核情况.取对数生长期H460和EC109细胞,分为单纯照射组、照射联合Tat-Smac N7组,单纯照射组给予0、2、4、6 Gy照射,照射联合Tat-Smac N7组中Tat-Smac N7的浓度为20 μmol/L,WST-1测定Tat-Smac N7的辐射增敏作用.取对数生长期H460和EC109细胞,分为对照组、Tat-Smac N7组、单纯照射组和照射联合Tat-Smac N7组,吸收剂量为4 Gy,Tat-Smac N7浓度为20 μmol/L,细胞流式分析仪测定细胞不同时间的细胞凋亡率.结果 Tat-Smac N7融合蛋白进入2种细胞系后2h可以有蓄积,且这种蓄积可延续到24 h,而Smac N7则不能进入细胞.Tat-Smac N7能够增强H460和EC109细胞的辐射敏感性(F=22.2、13.2,P＜0.05),照射联合Tat-Smac N7可明显增加辐射诱导的细胞凋亡率(24 h:F=9.32、5.86,P＜0.05;48 h:F =7.09、8.25,P＜0.05).Tat-Smac N7联合照射后凋亡诱导效应具有时间依赖性.结论 Tat-Smac N7融合肽可促进肿瘤细胞的辐射敏感性,作为一种新的Smac蛋白类似物,有望用于肿瘤的辐射增敏治疗.
목적 관찰Tat-Smac N7융합태대인폐암세포계H460화인식관암세포계EC109적복사증민작용,탐토기복사증민궤제.방법 취대수생장기H460화EC109세포,분위DAPI대조조、FITC-Smac N7화FITC-Tat-Smac N7조,형광현미경관찰량충세포불동시간약물입핵정황.취대수생장기H460화EC109세포,분위단순조사조、조사연합Tat-Smac N7조,단순조사조급여0、2、4、6 Gy조사,조사연합Tat-Smac N7조중Tat-Smac N7적농도위20 μmol/L,WST-1측정Tat-Smac N7적복사증민작용.취대수생장기H460화EC109세포,분위대조조、Tat-Smac N7조、단순조사조화조사연합Tat-Smac N7조,흡수제량위4 Gy,Tat-Smac N7농도위20 μmol/L,세포류식분석의측정세포불동시간적세포조망솔.결과 Tat-Smac N7융합단백진입2충세포계후2h가이유축적,차저충축적가연속도24 h,이Smac N7칙불능진입세포.Tat-Smac N7능구증강H460화EC109세포적복사민감성(F=22.2、13.2,P＜0.05),조사연합Tat-Smac N7가명현증가복사유도적세포조망솔(24 h:F=9.32、5.86,P＜0.05;48 h:F =7.09、8.25,P＜0.05).Tat-Smac N7연합조사후조망유도효응구유시간의뢰성.결론 Tat-Smac N7융합태가촉진종류세포적복사민감성,작위일충신적Smac단백유사물,유망용우종류적복사증민치료.
Objective To observe the radiosensitization effect of Tat-Smac N7 fusion peptide in EC109 and H460 cell lines,and to explore the mechanism of Tat-Smac N7 in radio sensitization.Methods H460 and EC109 cells were divided into DAPI group,and FITC-Smac N7 group,FITC-Tat-Smac N7 group.Fluorescence microscope was used to detect if the peptide had been entered into tumor cells at deferent times.H460 and EC109 cells were divided into radiation group and Tat-Smac N7 combined with radiation group.The cells were irradiated with 4 Gy γ-ray and the concentration of Tat-Smac N7 was 20 μmol/L.The proliferation of tumor cells was detected by WST-1 assay.Among control group,radiation group,Tat-Smac N7 group and Tat-Smac N7 combined with radiation group,apoptosis was detected by flow cytometry.Results Tat-Smac N7 could enter cells for 2-24 h,but Smac N7 couldn't.Tat-Smac N7 promoted the radio sensitization of H460 and EC109 cells obviously (F =22.2,13.2,P ＜ 0.05).The apoptosis of combined group was much higher than that of control (24 h:F =9.32,5.86,P ＜ 0.05; 48 h:F =7.09,8.25,P ＜0.05).The apoptosis in Tat-Smac N7 combined with radiation group varied with time-dependence.Conclusions Tat-Smac N7 fusion peptide showed remarkable radiosensitization in tumor cells.As a new Smac mimetic,Tat-Smac N7 was potential in radio sensitization therapy of tumor in the future.