中华放射学杂志
中華放射學雜誌
중화방사학잡지
Chinese Journal of Radiology
2015年
1期
65-68
,共4页
碘化油%脑栓塞%脂肪乳%动物实验
碘化油%腦栓塞%脂肪乳%動物實驗
전화유%뇌전새%지방유%동물실험
Iodized oil%Intracranial embolism%Fat emulsions%Animal experimentation
目的 探讨脂肪乳注射液对颅内碘油清除的作用.方法 12只家兔按随机数字表法分成2组,对照组、实验组各6只,均通过颈内动脉穿刺注入超液态碘油(0.06 ml/kg),CT扫描发现脑内有碘油沉积为造模成功.实验组造模成功后即刻经静脉注射脂肪乳(20 ml/kg),并于其后每隔24h,即术后24、48、72、96、120 h分别注射1次脂肪乳,共6次,并于上述时间点及术后144h各行1次头颅CT扫描.对照组不做治疗,并于相同时间点行CT扫描.选取碘油沉积最明显的0.1 cm×0.1 cm ROI测量CT值.2组间不同时点比较采用重复测量的方差分析,2组间相同时点比较采用两独立样本t检验,并观察动物的临床症状变化情况.结果 术后24、48、72、96、120、144h,对照组ROI CT值分别为(103.8±7.1)、(91.0±4.2)、(79.5±5.5)、(67.8±6.6)、(53.9±5.1)、(39.9±3.1)HU,实验组相应时间点ROICT值分别为(90.7±5.4)、(74.1±4.6)、(62.9±4.5)、(48.1±3.1)、(39.1±1.3)、(38.8±1.2)HU.重复测量的方差分析显示,2组间不同时间点CT值差异有统计学意义(F=201.30,P＜0.01).两独立样本t检验比较显示,2组间术后24～ 120 h相同时间点CT值的差异有统计学意义(t值分别为3.60、6.64、5.72、6.62、6.89,P值均＜0.01).造模成功后,所有动物均出现不同程度卒中症状,实验组临床症状完全消失较对照组提前24 h.结论 脂肪乳注射液能加速颅内碘油清除,本实验为临床应用脂肪乳治疗碘油脑栓塞提供了一定的理论依据.
目的 探討脂肪乳註射液對顱內碘油清除的作用.方法 12隻傢兔按隨機數字錶法分成2組,對照組、實驗組各6隻,均通過頸內動脈穿刺註入超液態碘油(0.06 ml/kg),CT掃描髮現腦內有碘油沉積為造模成功.實驗組造模成功後即刻經靜脈註射脂肪乳(20 ml/kg),併于其後每隔24h,即術後24、48、72、96、120 h分彆註射1次脂肪乳,共6次,併于上述時間點及術後144h各行1次頭顱CT掃描.對照組不做治療,併于相同時間點行CT掃描.選取碘油沉積最明顯的0.1 cm×0.1 cm ROI測量CT值.2組間不同時點比較採用重複測量的方差分析,2組間相同時點比較採用兩獨立樣本t檢驗,併觀察動物的臨床癥狀變化情況.結果 術後24、48、72、96、120、144h,對照組ROI CT值分彆為(103.8±7.1)、(91.0±4.2)、(79.5±5.5)、(67.8±6.6)、(53.9±5.1)、(39.9±3.1)HU,實驗組相應時間點ROICT值分彆為(90.7±5.4)、(74.1±4.6)、(62.9±4.5)、(48.1±3.1)、(39.1±1.3)、(38.8±1.2)HU.重複測量的方差分析顯示,2組間不同時間點CT值差異有統計學意義(F=201.30,P＜0.01).兩獨立樣本t檢驗比較顯示,2組間術後24～ 120 h相同時間點CT值的差異有統計學意義(t值分彆為3.60、6.64、5.72、6.62、6.89,P值均＜0.01).造模成功後,所有動物均齣現不同程度卒中癥狀,實驗組臨床癥狀完全消失較對照組提前24 h.結論 脂肪乳註射液能加速顱內碘油清除,本實驗為臨床應用脂肪乳治療碘油腦栓塞提供瞭一定的理論依據.
목적 탐토지방유주사액대로내전유청제적작용.방법 12지가토안수궤수자표법분성2조,대조조、실험조각6지,균통과경내동맥천자주입초액태전유(0.06 ml/kg),CT소묘발현뇌내유전유침적위조모성공.실험조조모성공후즉각경정맥주사지방유(20 ml/kg),병우기후매격24h,즉술후24、48、72、96、120 h분별주사1차지방유,공6차,병우상술시간점급술후144h각행1차두로CT소묘.대조조불주치료,병우상동시간점행CT소묘.선취전유침적최명현적0.1 cm×0.1 cm ROI측량CT치.2조간불동시점비교채용중복측량적방차분석,2조간상동시점비교채용량독립양본t검험,병관찰동물적림상증상변화정황.결과 술후24、48、72、96、120、144h,대조조ROI CT치분별위(103.8±7.1)、(91.0±4.2)、(79.5±5.5)、(67.8±6.6)、(53.9±5.1)、(39.9±3.1)HU,실험조상응시간점ROICT치분별위(90.7±5.4)、(74.1±4.6)、(62.9±4.5)、(48.1±3.1)、(39.1±1.3)、(38.8±1.2)HU.중복측량적방차분석현시,2조간불동시간점CT치차이유통계학의의(F=201.30,P＜0.01).량독립양본t검험비교현시,2조간술후24～ 120 h상동시간점CT치적차이유통계학의의(t치분별위3.60、6.64、5.72、6.62、6.89,P치균＜0.01).조모성공후,소유동물균출현불동정도졸중증상,실험조림상증상완전소실교대조조제전24 h.결론 지방유주사액능가속로내전유청제,본실험위림상응용지방유치료전유뇌전새제공료일정적이론의거.
Objective To evaluate the value of removing intracranial lipiodol by fat emulsion injection.Methods Twelve rabbits were randomly divided into two groups,control group(n=6) and experimental group(n=6).All rabbits were injected lipiodolvia the internal carotid artery(0.06 ml/kg).When lipiodol was found intracranially by CT scan,the model was considered to be successful.The rabbits were then injected with fat emulsions intravenously immediatedly following the CT and at intervals of 24 hours,for a total of 6 times(20 ml/kg).Subsequently,the experimental group of rabbits underwent head CT scan at that time and 144 hours later.The control group without treatment underwent head CT scan at the same time interval.The highest density of 0.01 cm2 was selected as region of interest and the CT value was measured.Comparison between the two groups at different times used repeated measurements of ANOVA.Same time points between the two groups were compared using the two independent-samplesttest.Changes of clinical symptoms were observed in rabbits.Results At 24,48,72,96,120 hours,144 hours post-treatment,the CT values of the ROI in the control group and the experimentalgroupwere(103.8 ±7.1),(91.0±4.2),(79.5 ±5.5),(67.8±6.6),(53.9±5.1),(39.9±3.1)HU respectively and(90.7-±5.4),(74.1±4.6),(62.9±4.5),(48.1±3.1),(39.1±1.3),(38.8± 1.2)HU respectively.The results of the repeated measurementsof ANOVA showed that the CT values difference of the two groups at different time was statistically significant(F=201.30,P＜0.01).The results of the two independent-samples t test showed that the CT values difference of 24 to 120 hours posttreatment of the two groups also was statistically significant(t=3.60,6.64,5.72,6.62,6.89,P＜0.05).After the intra-arterial injection of lipiodol,all animals had different degrees of stroke symptoms.Clinical symptoms disappeared earlier in the experimental group than the control group by 24 hours.Conclusion Fat emulsions can accelerate the intracranial lipiodolclearence.This study provides some theoretical basis for clinical treatment of cerebrallipiodol embolism.
