中华妇产科杂志
中華婦產科雜誌
중화부산과잡지
CHINESE JOUNAL OF OBSTETRICS AND GYNECOLOGY
2015年
1期
37-40
,共4页
张颖%周莉%王红月%段华%郭银树%成九梅
張穎%週莉%王紅月%段華%郭銀樹%成九梅
장영%주리%왕홍월%단화%곽은수%성구매
子宫肌层%肌细胞,平滑肌%结合带区%显微镜检查,电子
子宮肌層%肌細胞,平滑肌%結閤帶區%顯微鏡檢查,電子
자궁기층%기세포,평활기%결합대구%현미경검사,전자
Myometrium%Myocytes,smooth muscle%Junctional zone%Microscopy,electron
目的 探讨子宫结合带区(JZ)肌细胞的超微结构特点.方法 收集2010年8月至2013年8月在首都医科大学附属北京妇产医院妇科微创中心因子宫颈病变行子宫全切除术的绝经前患者共16例,其中增殖期及分泌期各8例.子宫切除后取JZ组织及子宫外周肌层组织进行电镜标本的制作,与外周肌层肌细胞相对比,电镜观察JZ肌细胞的超微结构,并对JZ肌细胞和细胞核的大小、细胞内肌丝/细胞质面积的比值及其周期性变化进行定量分析.结果 (1)JZ肌细胞的超微结构:与外周肌层肌细胞相比,JZ肌细胞的胞突多;JZ肌细胞内与细胞合成功能相关的细胞器成分,如线粒体、高尔基体、内质网等明显增多,而与细胞收缩功能相关的成分如密体及密斑等减少.(2)JZ肌细胞和细胞核的大小及其周期性变化:增殖期JZ肌细胞和外周肌层肌细胞的直径分别为(4.70±0.52)、(4.69±1.20) μm,两者比较,差异无统计学意义(P=0.987);分泌期JZ肌细胞和外周肌层肌细胞的直径分别为(3.75±0.36)、(4.92±0.51) μm,两者比较,差异有统计学意义(P=0.006).增殖期JZ肌细胞和外周肌层肌细胞核的直径分别为(3.24±0.41)、(2.90±0.62)μm,分泌期JZ肌细胞和外周肌层肌细胞核的直径分别为(2.44±0.27)、(2.92_±0.44) μm,两者分别比较,差异均无统计学意义(P=0.374,P=0.097).JZ肌细胞及细胞核的大小均随月经周期而发生明显的周期性变化(P＜0.05),而外周肌层肌细胞及细胞核的大小随月经周期均未发生明显的周期性变化(P＞0.05).(3)JZ肌细胞内肌丝/细胞质面积的比值及其周期性变化:增殖期、分泌期JZ肌细胞内肌丝/细胞质面积的比值分别为0.27±0.04、0.34±0.03,均显著低于相应期别的外周肌层肌细胞的0.49±0.03、0.54±0.03 (P=0.000,P=0.000).JZ肌细胞内肌丝/细胞质面积的比值也随月经周期而发生明显的周期性变化(P=0.029),而外周肌层肌细胞内肌丝/细胞质面积的比值随月经周期未发生明显的周期性变化(P=0.083).结论 与外周肌层肌细胞相比,JZ肌细胞中与合成功能相关的细胞器成分增加,而与收缩功能相关的细胞器成分减少;且超微结构特点在月经周期的不同时相呈现明显的周期性变化,这是其生理功能的结构基础.
目的 探討子宮結閤帶區(JZ)肌細胞的超微結構特點.方法 收集2010年8月至2013年8月在首都醫科大學附屬北京婦產醫院婦科微創中心因子宮頸病變行子宮全切除術的絕經前患者共16例,其中增殖期及分泌期各8例.子宮切除後取JZ組織及子宮外週肌層組織進行電鏡標本的製作,與外週肌層肌細胞相對比,電鏡觀察JZ肌細胞的超微結構,併對JZ肌細胞和細胞覈的大小、細胞內肌絲/細胞質麵積的比值及其週期性變化進行定量分析.結果 (1)JZ肌細胞的超微結構:與外週肌層肌細胞相比,JZ肌細胞的胞突多;JZ肌細胞內與細胞閤成功能相關的細胞器成分,如線粒體、高爾基體、內質網等明顯增多,而與細胞收縮功能相關的成分如密體及密斑等減少.(2)JZ肌細胞和細胞覈的大小及其週期性變化:增殖期JZ肌細胞和外週肌層肌細胞的直徑分彆為(4.70±0.52)、(4.69±1.20) μm,兩者比較,差異無統計學意義(P=0.987);分泌期JZ肌細胞和外週肌層肌細胞的直徑分彆為(3.75±0.36)、(4.92±0.51) μm,兩者比較,差異有統計學意義(P=0.006).增殖期JZ肌細胞和外週肌層肌細胞覈的直徑分彆為(3.24±0.41)、(2.90±0.62)μm,分泌期JZ肌細胞和外週肌層肌細胞覈的直徑分彆為(2.44±0.27)、(2.92_±0.44) μm,兩者分彆比較,差異均無統計學意義(P=0.374,P=0.097).JZ肌細胞及細胞覈的大小均隨月經週期而髮生明顯的週期性變化(P＜0.05),而外週肌層肌細胞及細胞覈的大小隨月經週期均未髮生明顯的週期性變化(P＞0.05).(3)JZ肌細胞內肌絲/細胞質麵積的比值及其週期性變化:增殖期、分泌期JZ肌細胞內肌絲/細胞質麵積的比值分彆為0.27±0.04、0.34±0.03,均顯著低于相應期彆的外週肌層肌細胞的0.49±0.03、0.54±0.03 (P=0.000,P=0.000).JZ肌細胞內肌絲/細胞質麵積的比值也隨月經週期而髮生明顯的週期性變化(P=0.029),而外週肌層肌細胞內肌絲/細胞質麵積的比值隨月經週期未髮生明顯的週期性變化(P=0.083).結論 與外週肌層肌細胞相比,JZ肌細胞中與閤成功能相關的細胞器成分增加,而與收縮功能相關的細胞器成分減少;且超微結構特點在月經週期的不同時相呈現明顯的週期性變化,這是其生理功能的結構基礎.
목적 탐토자궁결합대구(JZ)기세포적초미결구특점.방법 수집2010년8월지2013년8월재수도의과대학부속북경부산의원부과미창중심인자궁경병변행자궁전절제술적절경전환자공16례,기중증식기급분비기각8례.자궁절제후취JZ조직급자궁외주기층조직진행전경표본적제작,여외주기층기세포상대비,전경관찰JZ기세포적초미결구,병대JZ기세포화세포핵적대소、세포내기사/세포질면적적비치급기주기성변화진행정량분석.결과 (1)JZ기세포적초미결구:여외주기층기세포상비,JZ기세포적포돌다;JZ기세포내여세포합성공능상관적세포기성분,여선립체、고이기체、내질망등명현증다,이여세포수축공능상관적성분여밀체급밀반등감소.(2)JZ기세포화세포핵적대소급기주기성변화:증식기JZ기세포화외주기층기세포적직경분별위(4.70±0.52)、(4.69±1.20) μm,량자비교,차이무통계학의의(P=0.987);분비기JZ기세포화외주기층기세포적직경분별위(3.75±0.36)、(4.92±0.51) μm,량자비교,차이유통계학의의(P=0.006).증식기JZ기세포화외주기층기세포핵적직경분별위(3.24±0.41)、(2.90±0.62)μm,분비기JZ기세포화외주기층기세포핵적직경분별위(2.44±0.27)、(2.92_±0.44) μm,량자분별비교,차이균무통계학의의(P=0.374,P=0.097).JZ기세포급세포핵적대소균수월경주기이발생명현적주기성변화(P＜0.05),이외주기층기세포급세포핵적대소수월경주기균미발생명현적주기성변화(P＞0.05).(3)JZ기세포내기사/세포질면적적비치급기주기성변화:증식기、분비기JZ기세포내기사/세포질면적적비치분별위0.27±0.04、0.34±0.03,균현저저우상응기별적외주기층기세포적0.49±0.03、0.54±0.03 (P=0.000,P=0.000).JZ기세포내기사/세포질면적적비치야수월경주기이발생명현적주기성변화(P=0.029),이외주기층기세포내기사/세포질면적적비치수월경주기미발생명현적주기성변화(P=0.083).결론 여외주기층기세포상비,JZ기세포중여합성공능상관적세포기성분증가,이여수축공능상관적세포기성분감소;차초미결구특점재월경주기적불동시상정현명현적주기성변화,저시기생리공능적결구기출.
Objective To study the ultrastructural features of myocytes in uterine junctional zone (JZ).Methods From August 2010 to August 2013,there were 16 pre-menopause patients who suffered from cervical neoplasm to be performed hysterectomy.Samples of JZ and outer myometrium (OM) of hysterectomy specimens were collected.There were 8 specimens from the proliferative-phase and 8 specimens from the secretory-phase of endometrium.Ultrastructural features of JZ and OM were examined by using transmission electron microscopy and the related indices of myocytes were compared by using Student's t test.Results At JZ,there were more cytoplasmic process in the myocytes.The myocytes of JZ exhibited significant difference compared with those of OM.Firstly,the contractile structural components,such as the dense patches,dense bodies and the myofilaments were less abundant.In contrast,the perinuclear cell organelles were more distinct.The mitochondria,endoplasmic reticulum and Golgi apparatus were more prominent,denoting active protein synthesis.Secondly,the mean diameter of cell and nuclei demonstrated cyclic change.In proliferative phase of endometrium,the cell diameters of JZ and OM were (4.70_±0.52) and (4.69± 1.20) μm,respectively,which there were no significant difference(P=0.987).While in secretory phase,the cell diameters of JZ and OM were (3.75±0.36) and (4.92±0.51) μm,which there were significant difference (P=0.006).In proliferative phase,the nuclei diameters of JZ and OM were (3.24±0.41) and (2.90±0.62) μm,and in secretory phase,the nuclei diameters of JZ and OM were (2.44±0.27) and (2.92±0.44) μm.There were no significantly different in both phases (P=0.374,P=0.097).The diameters of cell and nuclei had cyclical changes (P ＜ 0.05).However,the cyclical changes were absent in OM (P＞ 0.05).Thirdly,the myofilaments/cytoplasm ratio of JZ in proliferative and secretory phases were 0.27±0.04 and 0.34±0.03,which were significantly less than those of OM in respective phases (0.49±0.03and 0.54±0.03;P=0.000,P=0.000).The myofilaments/cytoplasm ratio exhibited cyclical changes in JZ (P=0.029),but in OM,the cyclical changes were absent (P=0.083).Conclusions Compared with OM,ultrastructures associated with synthetic organelles are prominent,whereas the contractile organelles are reduced.And there are the cyclical changes in ultrastructural characteristics.The ultrastructural features of JZ are the basis of its physiology.
