CAJ | 학술논문

目的了解上海三家医院人鼻病毒(HRV)C组(HRV-C)在儿童急性呼吸道感染中的分子流行情况和临床特点.方法采用反转录聚合酶链反应(RT-PCR),对2012年1月至12月在上海交通大学医学院附属第三人民医院、上海交通大学医学院附属新华医院、上海市普陀区中心医院收集的271例急性呼吸道感染患儿的鼻咽抽吸物进行HRV-C基因检测、阳性产物测序、同源性和进化分析.计数资料采用卡方检验或核正卡方检验.结果 271份标本中共检出HRV-C阳性扩增产物8份,检出率为3.0％,次于HRV-A(5.9％);HRV-C核苷酸同源性为93％～100％,氨基酸同源性为97％～100％,其中5份存在混合感染,10月份检出较多,年龄均＜3岁,均诊断为下呼吸道感染,临床表现包括发热、阵发性咳嗽、咳痰、咽充血、肺部湿啰音、喘息等,无死亡病例.结论在上海三家医院急性呼吸道感染患儿中检出HRV-C,是次于HRV-A的流行基因型,其临床症状及诊断无特异性.
목적 료해상해삼가의원인비병독(HRV)C조(HRV-C)재인동급성호흡도감염중적분자류행정황화림상특점.방법 채용반전록취합매련반응(RT-PCR),대2012년1월지12월재상해교통대학의학원부속제삼인민의원、상해교통대학의학원부속신화의원、상해시보타구중심의원수집적271례급성호흡도감염환인적비인추흡물진행HRV-C기인검측、양성산물측서、동원성화진화분석.계수자료채용잡방검험혹핵정잡방검험.결과 271빈표본중공검출HRV-C양성확증산물8빈,검출솔위3.0％,차우HRV-A(5.9％);HRV-C핵감산동원성위93％～100％,안기산동원성위97％～100％,기중5빈존재혼합감염,10월빈검출교다,년령균＜3세,균진단위하호흡도감염,림상표현포괄발열、진발성해수、해담、인충혈、폐부습라음、천식등,무사망병례.결론 재상해삼가의원급성호흡도감염환인중검출HRV-C,시차우HRV-A적류행기인형,기림상증상급진단무특이성.
Objective To investigate the molecular epidemiology and clinical characteristics of human rhinovirus (HRV)-C in children with acute respiratory tract infection in three hospitals of Shanghai,China.Methods Between January 2012 and December 2012,271 nasopharyngeal aspiration (NPA) samples were collected from children who had been hospitalized for acute respiratory tract infection at Xinhua Hospital,Shanghai Jiaotong University School of Medicine,No.3 People Hospital Affiliated to Shanghai Jiaotong University School of Medicine and Putuo District Center Hospital,Shanghai.HRV-C was detected by the reverse transcription polymerase chain reaction (PCR) and all PCR positive products were sequenced.Phylogenetic analysis was performed.Enumeration data were analyzed using chi-square test.Results Eight out of 271 NPA samples were HRV-C positive,with the overall positive rate of 3.0％,ranking second to HRV-A(5.9％).Partial sequencing of the genes coding shared a 93％ to 100％ nucleotide identity with the HRV-C reference strains,and the amino acid identity was 97 ％ to 100％.Five of eight HRV-C positive samples were mix-infection.Positive specimens peaked in October.All HRV-C positive patients were ＜ 3 years old,with clinical diagnosis of lower respiratory tract infection.The clinical manifestations of HRV-C positive children included fever,paroxysmal productive cough,sputum,pharynx congestion,moist rale and wheezing.No patient died.Conclusions HRV-C is an important cause of respiratory tract infections in children in Shanghai region,which is only next to HRV-A.The clinical manifestations and diagnosis are both not specific.