CAJ | 학술논문

目的探讨不同剂量60Coγ射线照射对大鼠颌下腺凋亡及相关因子表达的影响.方法将Wistar大鼠随机分4组:(1)正常对照组;(2)放疗7.5Gy组;(3)放疗15 Gy组;(4)放疗22.5 Gy组.放疗组大鼠给予一次性γ射线辐射,每只辐射量按分组设定的剂量(7.5、15、22.5 Cy)给予.对照组未予照射.采用免疫组织化学法检测P53、Caspase-3表达情况以及原位切口末端标记(TUNEL)法检测细胞凋亡.结果与对照组比较,放疗组P53与Caspase-3的表达增强.Tunel染色显示的细胞凋亡主要见于导管细胞,随着放疗剂量增加,凋亡愈明显.结论 60Co γ射线照射可引起大鼠颌下腺早期细胞凋亡.60Co γ射线(7.5、15、22.5 Gy)照射诱导的颌下腺细胞凋亡有剂量-效应关系.
목적 탐토불동제량60Coγ사선조사대대서합하선조망급상관인자표체적영향.방법 장Wistar대서수궤분4조:(1)정상대조조;(2)방료7.5Gy조;(3)방료15 Gy조;(4)방료22.5 Gy조.방료조대서급여일차성γ사선복사,매지복사량안분조설정적제량(7.5、15、22.5 Cy)급여.대조조미여조사.채용면역조직화학법검측P53、Caspase-3표체정황이급원위절구말단표기(TUNEL)법검측세포조망.결과 여대조조비교,방료조P53여Caspase-3적표체증강.Tunel염색현시적세포조망주요견우도관세포,수착방료제량증가,조망유명현.결론 60Co γ사선조사가인기대서합하선조기세포조망.60Co γ사선(7.5、15、22.5 Gy)조사유도적합하선세포조망유제량-효응관계.
Objective To explore effects of different doses of 60Co γ-ray irradiation on apoptosis of rat submandibular gland dells.Methods Wistar rats were randomly divided into four different radiotherapy dosage groups (0 G y,7.5 G y,15 Gy,and 22.5 Gy).Rats in each radiotherapy groups were irradiated by the required dose of γ-ray irradiation (0 Gy,7.5 Gy,15 Gy,22.5 Gy) at one time.Immunohistochemistry method and TdT-mediated dUTP nick end labeling (TUNEL) methods were used to detect expression of P53,caspase-3,and apoptosis of submandibular gland dells in rats.Results The expression levels of P53 and caspase-3 of rat submandibular cells were significantly increased in different radiotherapy dosage groups (7.5 G y,15 Gy,and 22.5 Gy) relative the 0 Gy dosage group.The apoptotic cells were more commonly seen in duct cells in different radiotherapy dosage groups,and the apoptotic cells were gradually increased with the increased radiotherapy dosages.Conclusions 60 Co γ-ray irradiation could lead to apoptosis of rat submandibular gland in the early stage.There is a dose-effect relationship between 60Co γ-ray irradiation and induced apoptosis of rat submandibular gland cells.