CAJ | 학술논문

目的婴幼儿血管瘤是儿童最常见的实体肿瘤之一,该研究通过对婴幼儿血管瘤所有3个时期肥大细胞的标志物类胰蛋白酶和VEGF-A的检测,研究肥大细胞在婴幼儿血管瘤形成及消退中的作用.方法 22例增殖期、20例消退期和23例消退后期婴幼儿血管瘤石蜡标本分别通过免疫组织化学染色检测葡萄糖转运蛋白-1、类胰蛋白酶和血管内皮因子-A的表达;通过4',6'二脒基-2-苯吲哚(DAPI)对细胞核进行染色;观察类胰蛋白酶和VEGF-A表达情况,计算婴幼儿血管瘤所有3个时期类胰蛋白酶阳性细胞数,类胰蛋白酶阳性的肥大细胞表达VEGF-A的比例.结果 22例增殖期、20例消退期和23例消退后期婴幼儿血管瘤标本的Glut-1表达均为阳性.类胰蛋白酶表达阳性的肥大细胞位于血管内皮细胞组成的血管间隙组织.增殖期、消退期和消退后期血管瘤切片,每9个视野,类胰蛋白酶阳性细胞数分别为(27.26±4.10)个、(82.20±5.27)个和(44.59±3.07)个,不同时期比较,差异有统计学意义(P＜0.01).增殖期、消退期和消退后期中的类胰蛋白酶和VEGF-A表达均为阳性的细胞分别占100、(22.00±1.41)％和(13.00±1.38)％,不同时期比较,差异有统计学意义(P＜0.01).结论由肥大细胞分泌的VEGF-A在婴幼儿血管瘤增殖过程中起至关重要作用.
목적 영유인혈관류시인동최상견적실체종류지일,해연구통과대영유인혈관류소유3개시기비대세포적표지물류이단백매화VEGF-A적검측,연구비대세포재영유인혈관류형성급소퇴중적작용.방법 22례증식기、20례소퇴기화23례소퇴후기영유인혈관류석사표본분별통과면역조직화학염색검측포도당전운단백-1、류이단백매화혈관내피인자-A적표체;통과4',6'이미기-2-분신타(DAPI)대세포핵진행염색;관찰류이단백매화VEGF-A표체정황,계산영유인혈관류소유3개시기류이단백매양성세포수,류이단백매양성적비대세포표체VEGF-A적비례.결과 22례증식기、20례소퇴기화23례소퇴후기영유인혈관류표본적Glut-1표체균위양성.류이단백매표체양성적비대세포위우혈관내피세포조성적혈관간극조직.증식기、소퇴기화소퇴후기혈관류절편,매9개시야,류이단백매양성세포수분별위(27.26±4.10)개、(82.20±5.27)개화(44.59±3.07)개,불동시기비교,차이유통계학의의(P＜0.01).증식기、소퇴기화소퇴후기중적류이단백매화VEGF-A표체균위양성적세포분별점100、(22.00±1.41)％화(13.00±1.38)％,불동시기비교,차이유통계학의의(P＜0.01).결론 유비대세포분비적VEGF-A재영유인혈관류증식과정중기지관중요작용.
Objective To explore the function of mast cells in proliferation and involution of infantile hemangioma.Methods Paraffin-embedded sections of 22 proliferating,20 involuting and 23 involuted IH lesions were used to investigate the expression of tryptase,VEGF-A and GLUT-1 by immunostaining.The nuclei of all cells were stained by 4',6'-diamidino-2-phenylindole (DAPI).The expression of VEGF and tryptase were observed.The tryptase + mast cells and the percent of VEGF-A + cells in the whole tryptase + mast cells were calculated in all three phases of IH.Results The expression of Glut-1 in all sections of 22 proliferating,20 involuting and 23 involuted IH lesions were all positive.Mast cells,identified by their expression of tryptase,were located in the interstitium of vessels composed by vascular endothelial cells.The average numbers of tryptase + cells in each 9 views of proliferating,involuting and involuted IH were 27.26 ± 4.10,82.20 ± 5.27,44.59 ± 3.07,respectively,which difference is obviously (P＜0.01).100％,(22.00 ± 1.41) ％,(13.00 ± 1.38) ％ ofthese tryptase + cells also expressed VEGF,in proliferating,involuting and involuted IH,respectively,which was difference is also obviously (P＜0.01).Conclusions VEGF-A which was secreted by the most cells plays a vital role in the process of proliferation of IH.